Submitted:
16 July 2018
Posted:
16 July 2018
Read the latest preprint version here
Abstract
Minimal plasmids play an essential role in many intermediate steps in molecular biology. They can for example be used to assemble building blocks in synthetic biology or be used as intermediate cloning plasmids that are ideal for PCR-based mutagenesis methods. A small backbone also opens up for additional unique restriction enzyme cloning sites. Here we describe the generation of a ~1kb fully functional cloning plasmid with an extended multiple cloning site (MCS). To our knowledge, this is the smallest high-copy cloning vector ever described.
Keywords:
PCR
; recombination
; cloning
; engineering
; biotechnology
; synthetic biology
; synthetic nucleotide
; plasmids
; repository
; minimalism
; Escherichia coli
; mutagenesis
Copyright: This open access article is published under a Creative Commons CC BY 4.0 license, which permit the free download, distribution, and reuse, provided that the author and preprint are cited in any reuse.
