Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Engineering a Minimal 1185 Bp Cloning Vector from a Puc18 Plasmid Backbone with an Extended Multiple Cloning Site

Version 1 : Received: 16 July 2018 / Approved: 16 July 2018 / Online: 16 July 2018 (12:52:38 CEST)
Version 2 : Received: 12 October 2018 / Approved: 15 October 2018 / Online: 15 October 2018 (09:22:22 CEST)

A peer-reviewed article of this Preprint also exists.


Minimal plasmids play an essential role in many intermediate steps in molecular biology. They can for example be used to assemble building blocks in synthetic biology or be used as intermediate cloning plasmids that are ideal for PCR-based mutagenesis methods. A small backbone also opens up for additional unique restriction enzyme cloning sites. Here we describe the generation of a ~1kb fully functional cloning plasmid with an extended multiple cloning site (MCS). To our knowledge, this is the smallest high-copy cloning vector ever described.


PCR, recombination, cloning, engineering, biotechnology, synthetic biology, synthetic nucleotide, plasmids, repository, minimalism, Escherichia coli, mutagenesis


Biology and Life Sciences, Biochemistry and Molecular Biology

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