Preprint Article Version 1 This version is not peer-reviewed

Association of Homeobox B13 (HOXB13) Gene Variants with Prostate Cancer Risk in Iranian Population

Version 1 : Received: 7 July 2018 / Approved: 10 July 2018 / Online: 10 July 2018 (05:36:05 CEST)

How to cite: Roudi , R.; Nemati, H..; Moghadam, M.R.; Sotoudeh, M.; Narouie, B..; Shojaei, A. Association of Homeobox B13 (HOXB13) Gene Variants with Prostate Cancer Risk in Iranian Population. Preprints 2018, 2018070159 (doi: 10.20944/preprints201807.0159.v1). Roudi , R.; Nemati, H..; Moghadam, M.R.; Sotoudeh, M.; Narouie, B..; Shojaei, A. Association of Homeobox B13 (HOXB13) Gene Variants with Prostate Cancer Risk in Iranian Population. Preprints 2018, 2018070159 (doi: 10.20944/preprints201807.0159.v1).

Abstract

Background: Prostate cancer is a complex condition, in which both genetic and environmental factors concomitantly contribute to the tumor initiation and progression. Recently, HOXB13 has been proposed as a susceptibility gene for prostate cancer. Objective: So the current study was conducted to test the existence of potential variations in HOXB13 gene in Iranian men with prostate cancer (PCa) compared to benign prostatic hyperplasia (BPH) cases. Methods: The HOXB13 genetic status was screened in 51 samples, including 21 blood and tissue of PCa cases compared to 30 cases affected by BPH using PCR/sequencing. Then potential association between genomic DNA alterations in blood and tissue PCa specimens was investigated Results: Analysis of BPH tissues showed single nucleotide variations c.366C > T (rs) or c.513T > C (rs9900627) in exon 1, but not in exon 2. Evaluation of PCa tissues revealed two cases with both synonymous c.366C > T and c.513T > C variants and two cases with the synonymous c.366C > T variant in exon 1. The variants c.366C > T and c.513T > C simultaneously or separately were found blood samples of PCa patients. The novel variant c.127A > G in the exon 2 was detected in 1 PCa blood sample. Our analysis indicated a significant reciprocal correlation between HOXB13 mutation in tissue and blood in PCa cases (P=0.02). Conclusion: The variants in exon 2 of HOXB13 may influence the risk of prostate cancer. Also, evaluation of HOXB13 mutation may be considered as a novel marker for screening of PCa. Further investigations are warranted to evaluate the clinical significance of HOXB13 in Iranian population.

Subject Areas

prostate cancer; HOXB13; benign prostatic hyperplasia; variant; Iran

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