Molecular characterization of divergent closterovirus isolates infecting Ribes species

Igor Koloniuk; Thanuja Thekke-Veetil; Jean-Sébastien Reynard; Irena Mavrič Pleško; Jaroslava Přibylová; Justine Brodard; Isabelle Kellenberger; Tatiana Sarkisova; Josef Špak; Janja Lamovšek; Sebastien Massart; Thien Ho; Joseph Postman; Ioannis Tzanetakis

doi:10.20944/preprints201806.0258.v1

A peer-reviewed article of this Preprint also exists.

Koloniuk, I.; Thekke-Veetil, T.; Reynard, J.-S.; Mavrič Pleško, I.; Přibylová, J.; Brodard, J.; Kellenberger, I.; Sarkisova, T.; Špak, J.; Lamovšek, J.; Massart, S.; Ho, T.; Postman, J.D.; Tzanetakis, I.E. Molecular Characterization of Divergent Closterovirus Isolates Infecting Ribes Species. Viruses 2018, 10, 369. Koloniuk, I.; Thekke-Veetil, T.; Reynard, J.-S.; Mavrič Pleško, I.; Přibylová, J.; Brodard, J.; Kellenberger, I.; Sarkisova, T.; Špak, J.; Lamovšek, J.; Massart, S.; Ho, T.; Postman, J.D.; Tzanetakis, I.E. Molecular Characterization of Divergent Closterovirus Isolates Infecting Ribes Species. Viruses 2018, 10, 369.

Abstract

Five isolates of a new putative member of the genus Closterovirus, tentatively named blackcurrant leafroll associated virus 1 (BcLRaV-1), were identified in currant. The 17 kb long genome of BcLRaV-1 contained 10 open reading frames (ORFs). The replication associated polyprotein has two papain-like leader proteases, a methyltransferase, a helicase and an RNA-dependent RNA polymerase domain. Additional ORFs coded for heat shock protein 70 homolog, heat shock protein 90 homolog, two divergent copies of coat protein, and three accessory proteins without identifiable functions. Phylogenetic analysis showed that BcLRaV-1 is related to members of the genus Closterovirus and recombination analysis of the isolates showed clear evidences of intraspecies recombination.

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Molecular characterization of divergent closterovirus isolates infecting Ribes species

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