Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

The Production of Isoflavones by Genista tinctoria L. In Vitro Culture after Two Vanadium Compounds Application. Possible Transport Mechanism of Secondary Metabolites through Plasma Membrane

Milan Skalicky
* ORCID logo ,
Jan Kubes
* ,
Vaclav Hejnak
,
Lenka Tumova
,
Jaroslava Martinkova
,
Jan Martin
,
Helena Hnilickova
Version 1 : Received: 1 June 2018 / Approved: 4 June 2018 / Online: 4 June 2018 (10:13:25 CEST)

A peer-reviewed article of this Preprint also exists.

Skalicky, M.; Kubes, J.; Hejnak, V.; Tumova, L.; Martinkova, J.; Martin, J.; Hnilickova, H. Isoflavones Production and Possible Mechanism of Their Exudation in Genista tinctoria L. Suspension Culture after Treatment with Vanadium Compounds. Molecules 2018, 23, 1619. Skalicky, M.; Kubes, J.; Hejnak, V.; Tumova, L.; Martinkova, J.; Martin, J.; Hnilickova, H. Isoflavones Production and Possible Mechanism of Their Exudation in Genista tinctoria L. Suspension Culture after Treatment with Vanadium Compounds. Molecules 2018, 23, 1619.

Abstract

Family Fabaceae traditionally serves as food and herbal remedies source. Several plants are already used for menopausal symptoms treatment based on a presence of typical secondary metabolites, isoflavones. Beside soybean and clovers, isoflavones could be produced by other plants or in vitro cultures. This type of production can be further enhanced by elicitation that stimulates metabolites biosynthesis via stress reaction. Vanadium compounds have been already described as potential elicitors and the aim of this study was to determine an impact of NH4VO3 and VOSO4 solutions on isoflavones production in Genista tinctoria L. cell cultures. The significant increase of isoflavones content such as genistin, genistein or formononetin was measured in a nutrient medium or dry mass after NH4VO3 treatment for 24 or 48 hours. The possible transport mechanism of isoflavones was also evaluated. An incubation with different transport inhibitors prior elicitation took effect on isoflavones content in the medium. However, there was non-ended result for particular metabolites such as genistein and daidzein, where ABC or alternatively MATE proteins can participate. Possible elicitation by some inhibitors was also discussed as result of their pleiotropic effect. Despite this outcome, the determining of transport mechanism is important step for identification of specific transporter.

Keywords

heavy metals; dyer´s greenweed; elicitation; exudation

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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