Preprint Article Version 2 Preserved in Portico This version is not peer-reviewed

Salt Stress Induces Non-CG Methylation in Coding Regions of Barley Seedlings (Hordeum vulgare)

Moumouni Konaté
ORCID logo ,
Michael J. Wilkinson
* ORCID logo ,
Benjamin T. Mayne
,
Stephen M. Pederson
,
Eileen S. Scott
,
Bettina Berger
,
Carlos M. Rodriguez Lopez
*
Version 1 : Received: 24 April 2018 / Approved: 25 April 2018 / Online: 25 April 2018 (07:38:20 CEST)
Version 2 : Received: 14 June 2018 / Approved: 15 June 2018 / Online: 15 June 2018 (06:10:53 CEST)

A peer-reviewed article of this Preprint also exists.

Konate, M.; Wilkinson, M.J.; Mayne, B.T.; Pederson, S.M.; Scott, E.S.; Berger, B.; Rodriguez Lopez, C.M. Salt Stress Induces Non-CG Methylation in Coding Regions of Barley Seedlings (Hordeum vulgare). Epigenomes 2018, 2, 12. Konate, M.; Wilkinson, M.J.; Mayne, B.T.; Pederson, S.M.; Scott, E.S.; Berger, B.; Rodriguez Lopez, C.M. Salt Stress Induces Non-CG Methylation in Coding Regions of Barley Seedlings (Hordeum vulgare). Epigenomes 2018, 2, 12.

Abstract

Salinity can negatively impact crop growth and yield. Changes in DNA methylation are known to occur when plants are challenged by stress and have been associated with the regulation of stress-response genes. However, the role of DNA-methylation in moderating gene expression in response to salt stress has been relatively poorly studied among crops such as barley. Here, we assessed the extent of salt-induced alterations of DNA methylation in barley and their putative role in perturbed gene expression. Using Next Generation Sequencing, we screened the leaf and root methylomes of five divergent barley varieties grown under control and three salt concentrations, to seek genotype independent salt-induced changes in DNA methylation. Salt stress caused increased methylation in leaves but diminished methylation in roots with a higher number of changes in leaves than in roots, indicating that salt induced changes to global methylation are organ specific. Differentially Methylated Markers (DMMs) were mostly located in close proximity to repeat elements, but also in 1094 genes, of which many possessed gene ontology (GO) terms associated with plant responses to stress. Identified markers have potential value as sentinels of salt stress and provide a starting point to allow understanding of the functional role of DNA methylation in facilitating barley’s response to this stressor.

Keywords

epigenetics; differentially methylated markers (DMMs); LEAVES; roots; DNA methylation; salinity stress; barley

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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