Version 1
: Received: 20 March 2018 / Approved: 28 March 2018 / Online: 28 March 2018 (09:18:30 CEST)
How to cite:
El-Attar, A.; Aboul-Ata, A.; Persson, J.; Mazyad, H.; Harandi, A.M.; Olsson, O. Recombinant TMV Vector for Production of Highly Immunogenic Plant-Based Vaccine Antigens Against Herpes Simplex Virus Type 2. Preprints2018, 2018030236. https://doi.org/10.20944/preprints201803.0236.v1
El-Attar, A.; Aboul-Ata, A.; Persson, J.; Mazyad, H.; Harandi, A.M.; Olsson, O. Recombinant TMV Vector for Production of Highly Immunogenic Plant-Based Vaccine Antigens Against Herpes Simplex Virus Type 2. Preprints 2018, 2018030236. https://doi.org/10.20944/preprints201803.0236.v1
El-Attar, A.; Aboul-Ata, A.; Persson, J.; Mazyad, H.; Harandi, A.M.; Olsson, O. Recombinant TMV Vector for Production of Highly Immunogenic Plant-Based Vaccine Antigens Against Herpes Simplex Virus Type 2. Preprints2018, 2018030236. https://doi.org/10.20944/preprints201803.0236.v1
APA Style
El-Attar, A., Aboul-Ata, A., Persson, J., Mazyad, H., Harandi, A.M., & Olsson, O. (2018). Recombinant TMV Vector for Production of Highly Immunogenic Plant-Based Vaccine Antigens Against Herpes Simplex Virus Type 2. Preprints. https://doi.org/10.20944/preprints201803.0236.v1
Chicago/Turabian Style
El-Attar, A., Ali M. Harandi and Olof Olsson. 2018 "Recombinant TMV Vector for Production of Highly Immunogenic Plant-Based Vaccine Antigens Against Herpes Simplex Virus Type 2" Preprints. https://doi.org/10.20944/preprints201803.0236.v1
Abstract
A B S T R A C T Genital herpes disease is caused mainly by herpes simplex virus type 2 (HSV-2), which is sexually transmitted with a high prevalence in both developed and developing countries. No vaccine is currently available against genital herpes. In this study, we introduced and expressed two genes encoding HSV-2 glycoprotein D (gD) and VP16 protein and a GFP marker gene (control) in tobacco seedlings. Positive plant infection was assessed through characteristic tobacco mosaic virus disease symptoms on leaves and by monitoring the fluorescence emitted by the expressed GFP protein. Expression of the HSV-gD2 and VP16 antigens was verified by RT-PCR, ELISA and Western blot. As a proof of concept, the immunogenicity and the protection ability of the plant produced gD antigen was tested in a mouse model of genital herpes and compared to gD antigen produced in a mammalian expression system. This showed that the plant-gD preparation, when used in combination with a CpG oligodeoxynucleotide as adjuvant, was highly immunogenic and capable of inducing complete immunity to lethal vaginal HSV-2 challenge in mice. Thus, the data presented here may have implications for the development of a production system for highly immunogenic plant-based HSV-2 vaccine antigens.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
