Preprint Article Version 1 This version is not peer-reviewed

Recombinant TMV Vector for Production of Highly Immunogenic Plant-Based Vaccine Antigens Against Herpes Simplex Virus Type 2

Version 1 : Received: 20 March 2018 / Approved: 28 March 2018 / Online: 28 March 2018 (09:18:30 CEST)

How to cite: El-Attar, A.; Aboul-Ata, A.; Persson, J.; Mazyad, H.; Harandi, A.M.; Olsson, O. Recombinant TMV Vector for Production of Highly Immunogenic Plant-Based Vaccine Antigens Against Herpes Simplex Virus Type 2. Preprints 2018, 2018030236 (doi: 10.20944/preprints201803.0236.v1). El-Attar, A.; Aboul-Ata, A.; Persson, J.; Mazyad, H.; Harandi, A.M.; Olsson, O. Recombinant TMV Vector for Production of Highly Immunogenic Plant-Based Vaccine Antigens Against Herpes Simplex Virus Type 2. Preprints 2018, 2018030236 (doi: 10.20944/preprints201803.0236.v1).

Abstract

A B S T R A C T Genital herpes disease is caused mainly by herpes simplex virus type 2 (HSV-2), which is sexually transmitted with a high prevalence in both developed and developing countries. No vaccine is currently available against genital herpes. In this study, we introduced and expressed two genes encoding HSV-2 glycoprotein D (gD) and VP16 protein and a GFP marker gene (control) in tobacco seedlings. Positive plant infection was assessed through characteristic tobacco mosaic virus disease symptoms on leaves and by monitoring the fluorescence emitted by the expressed GFP protein. Expression of the HSV-gD2 and VP16 antigens was verified by RT-PCR, ELISA and Western blot. As a proof of concept, the immunogenicity and the protection ability of the plant produced gD antigen was tested in a mouse model of genital herpes and compared to gD antigen produced in a mammalian expression system. This showed that the plant-gD preparation, when used in combination with a CpG oligodeoxynucleotide as adjuvant, was highly immunogenic and capable of inducing complete immunity to lethal vaginal HSV-2 challenge in mice. Thus, the data presented here may have implications for the development of a production system for highly immunogenic plant-based HSV-2 vaccine antigens.

Subject Areas

Keywords: TMV, plant vaccine, génital herpes, HSV-2, gD, VP16, GFP, mouse model genital herpes

Readers' Comments and Ratings (1)

Comment 1
Received: 26 April 2018
Commenter: Fletcher
The commenter has declared there is no conflict of interests.
Comment: GOOD NEWS FOR PATIENTS INFECTED WITH HERPES SIMPLEX VIRUS (HSV) AND HIV / AIDS ... read

DR. MAGANA is the only traditional doctor who can cure HIV, HERPES SIMPLEX VIRUS 1&2, WART, HEPATITIS including other SKIN INFECTIONS... Using His 21 CURE HERBAL MEDICINE. Recently, a very close friend of mine published an article on how he got rid of his cold sores using the 21 CURE HERBAL MEDICINE by Dr.Magana, which he took for 21 days. I recommend your medicine to people infected or diagnosed with any type of infection. For those who need your contact information, you can simply write to them by EMAIL or WHATSAPP:
+2349051208634
maganasolutioncentre@gmail.com
PLEASE SHARE WITH FRIENDS AND PEOPLE YOU KNOW WHO NEEDS HELP.
+ Respond to this comment
Leave a public comment
Send a private comment to the author(s)
Rate this article
Views 0
Downloads 0
Comments 1
Metrics 0
Leave a public comment

×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.