Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Intestinal Microbial and Metabolite Profiling of Mice Fed with Dietary Glucose and Fructose

Version 1 : Received: 22 January 2018 / Approved: 23 January 2018 / Online: 23 January 2018 (05:31:27 CET)

How to cite: Silva, J.C.P.; Mota, M.; Martins, F.O.; Nogueira, C.; Gonçalves, T.; Carneiro, T.; Pinto, J.; Duarte, D.; Barros, A.S.; Jones, J.G.; Gil, A.M. Intestinal Microbial and Metabolite Profiling of Mice Fed with Dietary Glucose and Fructose. Preprints 2018, 2018010210. https://doi.org/10.20944/preprints201801.0210.v1 Silva, J.C.P.; Mota, M.; Martins, F.O.; Nogueira, C.; Gonçalves, T.; Carneiro, T.; Pinto, J.; Duarte, D.; Barros, A.S.; Jones, J.G.; Gil, A.M. Intestinal Microbial and Metabolite Profiling of Mice Fed with Dietary Glucose and Fructose. Preprints 2018, 2018010210. https://doi.org/10.20944/preprints201801.0210.v1

Abstract

Increased sugar intake is implicated in Type-2 diabetes and fatty liver disease. Mechanisms by which glucose and fructose components promote these conditions are unclear. We hypothesize that alterations in intestinal metabolite and microbiota profiles specific to each monosaccharide are involved. Two groups of six adult C57BL/6 mice were fed for 10-weeks with a diet where either glucose or fructose was the sole carbohydrate component (G and F, respectively). A third group was fed with normal chow (N). Fecal metabolites were profiled every 2-weeks by 1H NMR and microbial composition was analysed by real-time PCR (qPCR). Glucose tolerance was also periodically assessed. N, G and F mice had similar weight gains and glucose tolerance. Multivariate analysis of NMR profiles indicated that F mice were separated from both N and G, with decreased butyrate and glutamate and increased fructose, succinate, taurine, tyrosine and xylose. Compared to N and G, F mice showed a shift in microbe populations from gram-positive Lactobacillus spp. to gram-negative Enterobacteria species. Substitution of normal chow carbohydrate mixture by either pure glucose or fructose for 10 weeks did not alter adiposity or glucose tolerance. However, F G and N mice generated distinctive fecal metabolite signatures with incomplete fructose absorption as a dominant feature of F mice.

Keywords

fructose; intestinal microbiota; short-chain fatty acids; metabolic profiling

Subject

Biology and Life Sciences, Endocrinology and Metabolism

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.