Preprint Article Version 1 This version is not peer-reviewed

Zn2+-Loaded Cellulose Beads Stabilized by Chitosan and Prepared via Freeze-Drying for Removing Human Testosterone in Plasma

Version 1 : Received: 12 January 2018 / Approved: 16 January 2018 / Online: 16 January 2018 (05:04:16 CET)

How to cite: Yu, H.; Ke, H.; Chen, T.; Li, X.; Tan, S.; Zhou, L.; Wang, S. Zn2+-Loaded Cellulose Beads Stabilized by Chitosan and Prepared via Freeze-Drying for Removing Human Testosterone in Plasma. Preprints 2018, 2018010133 (doi: 10.20944/preprints201801.0133.v1). Yu, H.; Ke, H.; Chen, T.; Li, X.; Tan, S.; Zhou, L.; Wang, S. Zn2+-Loaded Cellulose Beads Stabilized by Chitosan and Prepared via Freeze-Drying for Removing Human Testosterone in Plasma. Preprints 2018, 2018010133 (doi: 10.20944/preprints201801.0133.v1).

Abstract

Immobilized metal ion affinity adsorbents have been widely used in separation technique to purify proteins. Due to the leakage of metal ion from the adsorbents, there is no metal ion affinity adsorbent for hemoperfusion has been applied to clinical trial. In this study, in order to prevent the leakage of Zn2+ loaded from cellulose beads based adsorbent, improve its stability and adsorption capacity for testosterone, Freeze-drying method was used to enhance the porosity of cellulose beads, improve the surface area of the cellulose beads and adsorption capacity for testosterone. Chitosan was used to coat the adsorbents for preventing the leakage of Zn2+ loaded and improve the adsorbent’s stability. Moreover, the factors affecting adsorption ability and some components in plasma were also investigated. The results indicate the adsorption ability of the adsorbent can be significantly improved by freeze-drying. After the adsorbent was coated with 0.02% chitosan solution, the highest adsorption percentage reached 48%. During adsorption, the Zn2+ concentration in plasma did not rise. In addition, the adsorption percentage for total proteins was below 15%. The results may be caused by the pore size and surface area of the adsorbent enlarged via freeze-drying, and the chitosan solution went into the pores and coated the outer and inner surface of the adsorbent. The adsorbent has a potential clinical application to remove testosterone in patients with recurrent and metastatic prostate cancer.

Subject Areas

testosterone; cellulose bead; chitosan; coating; zinc ion; freeze drying; adsorbent

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