Preprint Review Version 1 This version not peer reviewed

Label Free Biosensor Methods in Detection of Food Pathogens and Listeria monocytogenes

Version 1 : Received: 13 October 2017 / Approved: 13 October 2017 / Online: 13 October 2017 (17:15:10 CEST)

How to cite: Radhakrishnan, R.; Poltronieri, P. Label Free Biosensor Methods in Detection of Food Pathogens and Listeria monocytogenes. Preprints 2017, 2017100094 (doi: 10.20944/preprints201710.0094.v1). Radhakrishnan, R.; Poltronieri, P. Label Free Biosensor Methods in Detection of Food Pathogens and Listeria monocytogenes. Preprints 2017, 2017100094 (doi: 10.20944/preprints201710.0094.v1).

Abstract

Food pathogens contaminate food products that allow their growth on the shelf and also under refrigerated conditions. Therefore, it is of utmost importance to lower the limit of detection (LOD) of the method used and to obtain the results within hours to few days. Biosensor methods exploit the available technologies to individuate and provide an approximate quantification of the bacteria present in a sample. The main bottleneck of these methods depend on the aspecific binding to the surfaces and on a change in sensitivity when bacteria are in a complex food matrix in respect to bacteria in a liquid food sample. In this review we introduce Surface Plasmon Resonance (SPR), new advancements in SPR techniques, and Electrochemical Impedance Spectroscopy (EIS), as label-free biosensing technologies for the detection of L. monocytogenes in foods. The application of the two methods has made possible the detection of L. monocytogenes with LOD of 1 log CFU/mL. Further advancement are envisaged through the combination of biosensor methods with immunoseparation of bacteria from larger volumes.

Subject Areas

Listeria monocytogenes; label-free biosensors; Surface Plasmon Resonance (SPR); Electrochemical Impedance Spectroscopy (EIS)

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