Preprint Review Version 1 This version not peer reviewed

How to Clear HBV cccDNA: CRISPR/Cas9 Might Be Promising

Version 1 : Received: 10 October 2017 / Approved: 10 October 2017 / Online: 10 October 2017 (17:59:11 CEST)

How to cite: Qiu, Y.; Liu, Y.; Ren, W.; Ren, J. How to Clear HBV cccDNA: CRISPR/Cas9 Might Be Promising. Preprints 2017, 2017100065 (doi: 10.20944/preprints201710.0065.v1). Qiu, Y.; Liu, Y.; Ren, W.; Ren, J. How to Clear HBV cccDNA: CRISPR/Cas9 Might Be Promising. Preprints 2017, 2017100065 (doi: 10.20944/preprints201710.0065.v1).

Abstract

BACKGROUND: Chronic hepatitis B infected with Hepatitis B virus remains a major health concern worldwide. Despite standard interferon-α and nucleotide analogues have been shown to reduce the deterioration of liver disease among chronic hepatitis B patients, covalently closed circular DNA was still difficult to eradicate. METHODS: A literature search of Pubmed and Web of science was performed with the following key words: ‘CRISPR’, ‘CRISPR/Cas9’, ‘hepatitis B’, ‘HBV’, ‘chronic hepatitis B’ and ‘HBV cccDNA’. The information about CRISPR/Cas9 for the treatment of HBV cccDNA or hepatitis B was reviewed. RESULTS: CRISPR/Cas9 could treat hepatitis B through suppressing or clearing HBV cccDNA with different gRNAs. CONCLUSION: With the emergence of CRISPR/Cas9 (the RNA-guided clustered regulatory interspaced short palindromic repeats, CRISPR) editing technology, clearance of hepatitis B virus and better prevention of liver carcinoma seemed to be possible.

Subject Areas

hepatitis B virus; chronic hepatitis B; cccDNA; CRISPR/Cas9; gene therapy

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