Version 1
: Received: 10 October 2017 / Approved: 10 October 2017 / Online: 10 October 2017 (17:59:11 CEST)
How to cite:
Qiu, Y.; Liu, Y.; Ren, W.; Ren, J. How to Clear HBV cccDNA: CRISPR/Cas9 Might Be Promising. Preprints2017, 2017100065. https://doi.org/10.20944/preprints201710.0065.v1
Qiu, Y.; Liu, Y.; Ren, W.; Ren, J. How to Clear HBV cccDNA: CRISPR/Cas9 Might Be Promising. Preprints 2017, 2017100065. https://doi.org/10.20944/preprints201710.0065.v1
Qiu, Y.; Liu, Y.; Ren, W.; Ren, J. How to Clear HBV cccDNA: CRISPR/Cas9 Might Be Promising. Preprints2017, 2017100065. https://doi.org/10.20944/preprints201710.0065.v1
APA Style
Qiu, Y., Liu, Y., Ren, W., & Ren, J. (2017). How to Clear HBV cccDNA: CRISPR/Cas9 Might Be Promising. Preprints. https://doi.org/10.20944/preprints201710.0065.v1
Chicago/Turabian Style
Qiu, Y., Wen Ren and Jing Ren. 2017 "How to Clear HBV cccDNA: CRISPR/Cas9 Might Be Promising" Preprints. https://doi.org/10.20944/preprints201710.0065.v1
Abstract
BACKGROUND: Chronic hepatitis B infected with Hepatitis B virus remains a major health concern worldwide. Despite standard interferon-α and nucleotide analogues have been shown to reduce the deterioration of liver disease among chronic hepatitis B patients, covalently closed circular DNA was still difficult to eradicate. METHODS: A literature search of Pubmed and Web of science was performed with the following key words: ‘CRISPR’, ‘CRISPR/Cas9’, ‘hepatitis B’, ‘HBV’, ‘chronic hepatitis B’ and ‘HBV cccDNA’. The information about CRISPR/Cas9 for the treatment of HBV cccDNA or hepatitis B was reviewed. RESULTS: CRISPR/Cas9 could treat hepatitis B through suppressing or clearing HBV cccDNA with different gRNAs. CONCLUSION: With the emergence of CRISPR/Cas9 (the RNA-guided clustered regulatory interspaced short palindromic repeats, CRISPR) editing technology, clearance of hepatitis B virus and better prevention of liver carcinoma seemed to be possible.
Keywords
hepatitis B virus; chronic hepatitis B; cccDNA; CRISPR/Cas9; gene therapy
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
