Preprint Article Version 1 This version not peer reviewed

Molecular Characterization and Identification of Facilitative Glucose Transporter 2 (GLUT2) and Expression of Related Glycometabolism in Response to Different Starch Levels in Blunt Snout Bream (Megalobrama amblycephala)

Version 1 : Received: 22 May 2017 / Approved: 22 May 2017 / Online: 22 May 2017 (06:12:21 CEST)

How to cite: Liang, H.; Wilson-Arop, O.; Mi, H.; J, K.; Ge, X.; Ren, M. Molecular Characterization and Identification of Facilitative Glucose Transporter 2 (GLUT2) and Expression of Related Glycometabolism in Response to Different Starch Levels in Blunt Snout Bream (Megalobrama amblycephala). Preprints 2017, 2017050158 (doi: 10.20944/preprints201705.0158.v1). Liang, H.; Wilson-Arop, O.; Mi, H.; J, K.; Ge, X.; Ren, M. Molecular Characterization and Identification of Facilitative Glucose Transporter 2 (GLUT2) and Expression of Related Glycometabolism in Response to Different Starch Levels in Blunt Snout Bream (Megalobrama amblycephala). Preprints 2017, 2017050158 (doi: 10.20944/preprints201705.0158.v1).

Abstract

Facilitative glucose transporters (GLUT) are transmembrane transporter of proteins involved in glucose transport across the plasma membrane. To date, there is no information about glucose transporter 2 (GLUT2) in blunt snout bream (Megalobram aamblycephala). In this study, GLUT2 were cloned and characterized from blunt snout bream, and its-expression in response to diets with different carbohydrate levels (17.1%; 21.8%; 26.4%; 32.0%; 36.3% and 41.9% of dry matter). In this study, the full-length cDNA of GLUT2 was 2577 bp, including a 5’-untranslated region (UTR) of 73 bp, a 3’-UTR of 992 bp, and an open reading frame of 1512 bp, encoding a polypeptide of 503 amino acids with predicted molecular weight of 55.046 kDa and theoretical isoelectric point of 7.52. GLUT2 has twelve across the membrane area locating at 7-29; 71-93; 106-123; 133-155; 168-190; 195-217; 282-301; 316-338; 345-367; 377-399; 412-434; 438-460 aimo acids respectively. Conservative structure domains located at 12-477 aimo acids belonging to sugar porter family major facilitator superfamily (MFS) transporter. The blunt snout bream GLUT2 showed high identity to their orthologs in other fish species and mammals. Quantitative real-time (qRT)-PCR assays revealed that GLUT2 expression was high in the liver, intestine and kidney; highest in the liver. Compared with the control group (17.1%), high dietary carbohydrate levels (32.0%; 36.3% and 41.9%) resulted in high plasma glucose at 3h after feeding, but high plasma glucose were back to basal at 24h after feeding. Furthermore, high dietary carbohydrate levels significantly improved the glycolysis and inhibitied gluconeogenesis by augmentation of GK and PK expression, inhibition of PEPCK and G6P mRNA levels (P<0.05). However, GLUT2; GK; PK; PEPCK and G6P mRNA levels were back to basal.

Subject Areas

blunt snout bream (megalobrama amblycephala); glucose transporter 2; glycometabolism; starch

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