PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Effects of Biomimetic Shear Stress by Self-Assembled Gelatin Oleic Nanoparticles Containing Paclitaxel on the Behavior of Cancer Cell Lines in a Microfluidic System
Version 1
: Received: 3 April 2017 / Approved: 4 April 2017 / Online: 4 April 2017 (10:59:02 CEST)
How to cite:
Kang, T.; Tran, T.T.T.; Oh, K.T.; Choi, H.; Lee, B. Effects of Biomimetic Shear Stress by Self-Assembled Gelatin Oleic Nanoparticles Containing Paclitaxel on the Behavior of Cancer Cell Lines in a Microfluidic System. Preprints2017, 2017040023. https://doi.org/10.20944/preprints201704.0023.v1
Kang, T.; Tran, T.T.T.; Oh, K.T.; Choi, H.; Lee, B. Effects of Biomimetic Shear Stress by Self-Assembled Gelatin Oleic Nanoparticles Containing Paclitaxel on the Behavior of Cancer Cell Lines in a Microfluidic System. Preprints 2017, 2017040023. https://doi.org/10.20944/preprints201704.0023.v1
Kang, T.; Tran, T.T.T.; Oh, K.T.; Choi, H.; Lee, B. Effects of Biomimetic Shear Stress by Self-Assembled Gelatin Oleic Nanoparticles Containing Paclitaxel on the Behavior of Cancer Cell Lines in a Microfluidic System. Preprints2017, 2017040023. https://doi.org/10.20944/preprints201704.0023.v1
APA Style
Kang, T., Tran, T.T.T., Oh, K.T., Choi, H., & Lee, B. (2017). Effects of Biomimetic Shear Stress by Self-Assembled Gelatin Oleic Nanoparticles Containing Paclitaxel on the Behavior of Cancer Cell Lines in a Microfluidic System. Preprints. https://doi.org/10.20944/preprints201704.0023.v1
Chicago/Turabian Style
Kang, T., Han-Gon Choi and Beom-Jin Lee. 2017 "Effects of Biomimetic Shear Stress by Self-Assembled Gelatin Oleic Nanoparticles Containing Paclitaxel on the Behavior of Cancer Cell Lines in a Microfluidic System" Preprints. https://doi.org/10.20944/preprints201704.0023.v1
Abstract
Fluid flow in human body is generally known to influence a variety of cellular behaviors. Different nanoparticle properties as well as cell type, interaction with other cells and cellular environments also show significant effect on nanoparticle uptake and drug efficacy. The aim of this study was to evaluate the effect of shear stress on cellular behaviors of biocompatible and biodegradable nanoparticles to cancer cells (A549 cell lines) in a biomimetic microfluidic system. We prepared a gelatin-oleic conjugate (GOC) as an amphiphilic biomaterial to prepare self-assembled gelatin-oleic nanoparticles (GON). Coumarin-6 and paclitaxel were used as the fluorescence marker and model drug, respectively, and were loaded into GONs by incubation (C-GONs; PTX-GONs). Additionally, we evaluated the cellular uptake of fluorescence labeled C-GONs and the drug efficacy of PTX-GONs. The cellular uptake of C-GONs by A549 cells in the absence of shear stress revealed that the mean fluorescence intensity was slightly decreased compared to that in the presence of shear stress. The results also indicated that negatively charged PTX-GONs had a lower cancer killing effect under dynamic conditions than that under static conditions. It also suggested that fluidic shear stress did not significantly affect drug uptake and efficiency in case of PTX-GONs. The cellular interactions between nanoparticles and cells in drug delivery should be carefully examined according to the physicochemical properties of nanoparticles such as the type of materials, size and mainly surface charge in a biomimetic microfluidic condition.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
