Version 1
: Received: 23 March 2017 / Approved: 23 March 2017 / Online: 23 March 2017 (09:33:31 CET)
How to cite:
Ou, Z.; Xu, J.; Niu, Y.; Cui, J. Preparation of Interface-Assembled Carbonyl Reductase and Its Application in Synthesis of S-Licarbazepine in Toluene/Tri-HCl Buffer Biphasic System. Preprints2017, 2017030180. https://doi.org/10.20944/preprints201703.0180.v1
Ou, Z.; Xu, J.; Niu, Y.; Cui, J. Preparation of Interface-Assembled Carbonyl Reductase and Its Application in Synthesis of S-Licarbazepine in Toluene/Tri-HCl Buffer Biphasic System. Preprints 2017, 2017030180. https://doi.org/10.20944/preprints201703.0180.v1
Ou, Z.; Xu, J.; Niu, Y.; Cui, J. Preparation of Interface-Assembled Carbonyl Reductase and Its Application in Synthesis of S-Licarbazepine in Toluene/Tri-HCl Buffer Biphasic System. Preprints2017, 2017030180. https://doi.org/10.20944/preprints201703.0180.v1
APA Style
Ou, Z., Xu, J., Niu, Y., & Cui, J. (2017). Preparation of Interface-Assembled Carbonyl Reductase and Its Application in Synthesis of S-Licarbazepine in Toluene/Tri-HCl Buffer Biphasic System. Preprints. https://doi.org/10.20944/preprints201703.0180.v1
Chicago/Turabian Style
Ou, Z., Yangping Niu and Jian Cui. 2017 "Preparation of Interface-Assembled Carbonyl Reductase and Its Application in Synthesis of S-Licarbazepine in Toluene/Tri-HCl Buffer Biphasic System" Preprints. https://doi.org/10.20944/preprints201703.0180.v1
Abstract
S-licarbazepine was prepared by asymmetric reduction of oxcarbazepine with interface-assembled carbonyl reductase at the interface of oil/water biphasic system. The carbonyl reductase was conjugated with polystyrene in the surface of toluene/Tris-HCl biphasic system and formed a surfactant-like structure. The conversion and enantiometric excess of S-licarbazepine reached 97.39% and 99.6% when 3.97 mmol/L oxcarbazepine was reduced by interface-assembled carbonyl reductase with 60 g/L ethanol as co-substrate in toluene/Tris-HCl(12.5:10) biphasic system at 30 °C, 180 rpm for 6 h.
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
