Preprint Article Version 1 This version is not peer-reviewed

Preparation of Interface-Assembled Carbonyl Reductase and Its Application in Synthesis of S-Licarbazepine in Toluene/Tri-HCl Buffer Biphasic System

Version 1 : Received: 23 March 2017 / Approved: 23 March 2017 / Online: 23 March 2017 (09:33:31 CET)

How to cite: Ou, Z.; Xu, J.; Niu, Y.; Cui, J. Preparation of Interface-Assembled Carbonyl Reductase and Its Application in Synthesis of S-Licarbazepine in Toluene/Tri-HCl Buffer Biphasic System. Preprints 2017, 2017030180 (doi: 10.20944/preprints201703.0180.v1). Ou, Z.; Xu, J.; Niu, Y.; Cui, J. Preparation of Interface-Assembled Carbonyl Reductase and Its Application in Synthesis of S-Licarbazepine in Toluene/Tri-HCl Buffer Biphasic System. Preprints 2017, 2017030180 (doi: 10.20944/preprints201703.0180.v1).

Abstract

S-licarbazepine was prepared by asymmetric reduction of oxcarbazepine with interface-assembled carbonyl reductase at the interface of oil/water biphasic system. The carbonyl reductase was conjugated with polystyrene in the surface of toluene/Tris-HCl biphasic system and formed a surfactant-like structure. The conversion and enantiometric excess of S-licarbazepine reached 97.39% and 99.6% when 3.97 mmol/L oxcarbazepine was reduced by interface-assembled carbonyl reductase with 60 g/L ethanol as co-substrate in toluene/Tris-HCl(12.5:10) biphasic system at 30 °C, 180 rpm for 6 h.

Subject Areas

Biotransformation; interface-assembling carbonyl reductase; oxcarbazepine; polystyrene; S-licarbazepine

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