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Preprint ARTICLE | doi:10.20944/preprints201610.0005.v1

Socio Environmental and Haematological Profile of Landfill Residents (São Jorge Landfill – Sao Paulo – Brazil)

Vivianni Wanderley, Fernando Luiz Affonso Fonseca, André Vala Quiaios, José Nuno Domingues, Susana Paixão, João Figueiredo, Ana Ferreira, Cleonice de Almeida Pinto, Odair Ramos da Silva, Rogério Alvarenga, Amaury Machi Junior, Eriane Justo Luiz Savóia, Rodrigo Daminello Raimundo

Subject: Medicine And Pharmacology, Hematology Keywords: Landfill, Waste, Socio-environmental impact, Hematologic diseases

Online: 4 October 2016 (09:03:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201705.0037.v1

Selection of an Animal Model for the Production of Leukocyte-Fibrin Rich Platelet Membranes (L-PRF); Standardized Protocol Proposed for Clinical Use and the Use of L-PRF Wound Box^®

Alessandro Crisci, Domenico Lombardi, Edda Serra, Guido Lombardi, Federica Cardillo, Michela Crisci

Subject: Medicine And Pharmacology, Hematology Keywords: autologous; buffy coat; growth factor level; platelet-rich fibrin; thrombocyte concentrate

Online: 4 May 2017 (08:37:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201709.0100.v1

Cut-off Values of Hematologic Parameters to Predict the Number of Alpha Genes Deleted in Subjects with Deletional Alpha Thalassemia

Diego Velasco-Rodríguez, Carlos Blas, Juan-Manuel Alonso-Domínguez, Gala Vega, Carlos Soto, Aránzazu García-Raso, Pilar Llamas-Sillero

Subject: Medicine And Pharmacology, Hematology Keywords: alpha; thalassemia; deletional; cut-off; number of genes; microcytic anemia; differential diagnosis

Online: 21 September 2017 (04:47:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201807.0422.v1

Saliva and Serum Ghrelin and Obestatin in Iron Deficiency Anemia Patients

Kader Ugur, Suleyman Aydin, Emir Donder, İbrahim Sahin, Meltem Yardim, Mehmet Kalayci, Nevzat Gozel, Ramazan Ulu, Muhammed Sait Dag, Murat Sarikaya

Subject: Medicine And Pharmacology, Hematology Keywords: iron deficiency anemia; obestatin; ghrelin

Online: 23 July 2018 (12:34:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202006.0001.v1

Increased Zinc and Albumin but Lowered Copper in Children with Transfusion-dependent Thalassemia

Zainab Alhillawi, Hussein Al-Hakeim, Shatha Moustafa, Michael Maes

Subject: Medicine And Pharmacology, Hematology Keywords: Copper; transfusion-dependent thalassemia; zinc; oxidative stress; antioxidants; biomarkers

Online: 2 June 2020 (09:21:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202302.0467.v1

Iron Deficiency and Polycythemia

Rova Malala Fandresena Randrianarisoa, Herveat Ramanandafy, Alexandre Mania, Hélène Monjanel, Sébastien Trouillier

Subject: Medicine And Pharmacology, Hematology Keywords: Diagnostic; Iron deficiency; Polycythemia; Polycythemia vera; Secondary polycythemia

Online: 27 February 2023 (09:36:45 CET)

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Preprint REVIEW | doi:10.20944/preprints201809.0435.v1

Targeting Oncogenic Signaling in Mutant FLT3 Acute Myeloid Leukemia: the Path to Least Resistance

Dilana Staudt, Heather Murray, Frank Alvaro, Anoop Enjeti, Nicole Verrills, Matthew D. Dun

Subject: Medicine And Pharmacology, Hematology Keywords: Acute Myeloid Leukemia; FLT3; Tyrosine kinase inhibitors; resistance

Online: 21 September 2018 (10:28:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202006.0275.v1

Analysis of the Primary Presenting Symptoms and Hematological Findings of COVID-19 Patients in Bangladesh

Abu Taiub Mohammed Mohiuddin Chowdhury, Md Rezaul Karim, H.M. Hamidullah Mehedi, Mohammad Shahbaz, Md Wazed Chowdhury, Guo Dan, Shuixiang He

Subject: Medicine And Pharmacology, Hematology Keywords: SARS-CoV-2; COVID-19; real-time RT-PCR; COVID-19 symptoms; COVID-19 hematological findings; Bangladesh

Online: 21 June 2020 (14:47:03 CEST)

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Preprint REVIEW | doi:10.20944/preprints202309.1180.v1

Immunotherapy of Multiple Myeloma: Current Status as Prologue to the Future

Hanley N. Abramson

Subject: Medicine And Pharmacology, Hematology Keywords: myeloma; daratumumab; bispecific antibodies; chimeric antigen receptor T-cells; immunotherapy

Online: 19 September 2023 (05:28:58 CEST)

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Preprint REVIEW | doi:10.20944/preprints202107.0203.v1

Polymorphisms of Genes Related to Phase-II Metabolism and the Resistance of Clopidogrel

Abdullah Alkattan, Ahmed alkhalifah, Eman Alsalameen, Fatimah Alghanim, Nashwa Radwan

Subject: Medicine And Pharmacology, Hematology Keywords: Polymorphism; genes; phase-II metabolism; glutathione; clopidogrel resistance

Online: 8 July 2021 (13:30:31 CEST)

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Working Paper ARTICLE

Diagnosis of Inherited Platelet disorders on a Blood Smear

Carlo Zaninetti, Andreas Greinacher

Subject: Medicine And Pharmacology, Hematology Keywords: inherited platelet disorders; hereditary thrombocytopenias; blood smear; immunofluorescence; bleeding tendency

Online: 20 January 2020 (09:50:59 CET)

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Preprint REVIEW | doi:10.20944/preprints202006.0062.v1

A Narrative Review on Evaluation of Hypercoagulability State in Severe COVID-19 Patients with Background Risk Factors

Minoosh Moghimi, Kasra Khodadadi, Yousef Mortazavi

Subject: Medicine And Pharmacology, Hematology Keywords: Covid19; DIC; coagulopathy; Sars Covid; blood coagulation disorder

Online: 7 June 2020 (07:41:24 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202002.0454.v1

Prevalence of Blood Groups in Eastern UP Population

Pradeep Kumar, Vandana Rai

Subject: Medicine And Pharmacology, Hematology Keywords: ABO blood groups; antigen; allele frequency; phenotype

Online: 29 February 2020 (08:26:18 CET)

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Preprint ARTICLE | doi:10.20944/preprints202209.0114.v1

Assessment of Knowledge, Attitude, and Practice toward Blood Transfusion among Healthcare Providers in Qatar

Danial Hassan, Abdulqadir J Nashwan, Mohamed Yassin

Subject: Medicine And Pharmacology, Hematology Keywords: Blood Transfusion; Knowledge; Attitude; Practice; Healthcare Providers

Online: 8 September 2022 (03:02:03 CEST)

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Preprint REVIEW | doi:10.20944/preprints202304.0482.v1

The Black Hole: CAR T cell Therapy in AML

Erden Atilla, Karim Benabdellah

Subject: Medicine And Pharmacology, Hematology Keywords: Acute Myeloid Leukemia, cellular therapies, chimeric antigen T cells, T cell receptor T cells, CAR-NK cells, RNAseq

Online: 18 April 2023 (04:22:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202209.0299.v1

Blood Groups (ABO/Rh) And Sociodemographic and Clinical Profile among Patients with Leprosy in Angola

Euclides Nenga Manuel Sacomboio, Tomásia Oliveira Muhongo, Adelino Tchilanda Tchivango, Edson Kuatelela Cassinela, Silvana da Rocha Silveira, Mauricio Da Costa, Carlos Alberto Pinto de Sousa, Cruz Sebastião Sebastião, Eduardo Ekundi Valentim

Subject: Medicine And Pharmacology, Hematology Keywords: leprosy; ABO/Rh blood group; Clinical; Angola

Online: 20 September 2022 (09:28:22 CEST)

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Preprint REVIEW | doi:10.20944/preprints201908.0199.v1

Concentrations of PCDD/Fs in Human Blood: A Review of Data from the Current Decade

Montse Marquès, Jose L Domingo

Subject: Medicine And Pharmacology, Hematology Keywords: polychlorinated dibenzo-p-dioxins (PCDDs); polychlorinated dibenzo-p-furans (PCDFs); human exposure; blood samples; dietary intake; risks

Online: 20 August 2019 (04:20:00 CEST)

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Preprint REVIEW | doi:10.20944/preprints202109.0127.v1

Is 6-Shogaol a Beneficial Phytochemical for Patients with Lower-Risk Myelodysplastic Syndrome? A Narrative Review

Soo Liang Ooi, Ron Campbell, Sok Cheon Pak, Terry Golombick, Arumugam Manoharan, Raj Ramakrishna, Vladimir Badmaev, Janet Schloss

Subject: Medicine And Pharmacology, Hematology Keywords: Zingiber officinale; 6-Shogaol; 6-gingerol; hematopoiesis; hepcidin; anaemia

Online: 7 September 2021 (12:08:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201910.0283.v1

Concentrations of C-Reative Protein and Leukocytes as Predictors of Adverse Health Factors in the Period of 30 Days Post-Hospital Discharge

Nayara Araújo dos Reis, Rosalina Aparecida Partezani Rodrigues, Darlene Mara dos Santos Tavares, Jack Roberto Silva Fhon, Jair Sindra Virtuoso Júnior

Subject: Medicine And Pharmacology, Hematology Keywords: chronic inflammation; biomarker panels; leukocyte count; C-reactive protein; related syndromes and pathologies; risk assessment; screening programmes; ageing; elderly's health

Online: 25 October 2019 (04:16:43 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202304.0616.v1

Circulating CD34+/CD38-/CD26+ Leukemia Stem Cells along Chronic Myeloid Leukemia progression: differences between Chronic, Accelerated and Blast Phase

Paola Pacelli, Anna Sicuranza, Elena Bestoso, Alessandra Iurlo, Elisabetta Abruzzese, Adele Santoni, Margherita Malchiodi, Cristina Marzano, Elisabetta Zappone, Donatella Raspadori, Monica Bocchia

Subject: Medicine And Pharmacology, Hematology Keywords: CP-CML; Accelerated Phase (AP); Blast Phase (BP); CD26+LSCs

Online: 20 April 2023 (07:31:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202009.0322.v1

Platelet Function and Microvesicles Generation in Patients with Hemophilia A

Antonia Melero-Amor, Paola Romecín, David Iyú, David García-Bernal, Esther García Navarro, José M. Moraleda, Joaquin Garcia-Estañ, Faustino García-Candel, Noemí M. Atucha

Subject: Medicine And Pharmacology, Hematology Keywords: Hemophilia A; Prophylaxis; Factor VIII; Platelets; Microvesicles; Calcium

Online: 14 September 2020 (16:12:08 CEST)

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Preprint REVIEW | doi:10.20944/preprints202108.0448.v2

Protein Biomarkers in Blood Reflect the Interrelationships between Stroke Outcome, Inflammation, Coagulation, Adhesion, Senescence and Cancer

Georg Fuellen, Jan Böhmert, Larissa Henze, Daniel Palmer, Uwe Walter, Soyoung Lee, Clemens Schmitt, Henrik Rudolf, Axel Kowald

Subject: Medicine And Pharmacology, Hematology Keywords: stroke; cellular senescence; coagulation; adhesion

Online: 14 October 2021 (15:21:58 CEST)

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Preprint REVIEW | doi:10.20944/preprints202307.1468.v1

Atypical Chronic Lymphocytic Leukemia—The Current Status

Tadeusz Robak, Anna Krawczyńska, Barbara Cebula-Obrzut, Marta Urbaniak, Elżbieta Iskierka-Jażdżewska, Paweł Robak

Subject: Medicine And Pharmacology, Hematology Keywords: CLL; atypical CLL; CD5; CD23; CLL differential diagnosis.

Online: 21 July 2023 (09:07:06 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.1898.v1

First-line Treatment of the Older Patients with CLL: A New Approach in the Chemo-free Era

Antonio Urso, Francesco Cavazzini, Maria Pia Ballardini, Silvia Gambara, Sara Consolo, Gian Matteo Rigolin, Antonio Cuneo

Subject: Medicine And Pharmacology, Hematology Keywords: chronic lymphocytic leukemia; older patient; ibrutinib; acalabrutinib; zanubrutinib; venetoclax; sustainabiity

Online: 27 June 2023 (12:04:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202110.0404.v1

The 4-Year Experience with Implementation and Routine Use of Pathogen Inactivation in a Brazilian Hospital

Roberta Maria Fachini, Rita Fontão-Wendel, Ruth Achkar, Patrícia Scuracchio, Mayra Brito, Marcelo Amaral, Silvano Wendel

Subject: Medicine And Pharmacology, Hematology Keywords: pathogen reduction; blood safety; platelet transfusion; INTERCEPT; plasma

Online: 27 October 2021 (12:27:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202204.0058.v2

Assessment of PVS Filtering Methods Using a Three-Dimensional Computational Model

Jose Bernal, Maria Valdés-Hernández, Javier Escudero, Roberto Duarte, Lucia Ballerini, Mark Bastin, Ian Deary, Michael Thrippleton, Rhian Touyz, Joanna Wardlaw

Subject: Medicine And Pharmacology, Hematology Keywords: Digital reference object; Perivascular spaces; Spatio-temporal imaging artefacts; Perivascular space enhancement; Cerebral small vessel disease

Online: 3 August 2022 (11:12:43 CEST)

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Preprint REVIEW | doi:10.20944/preprints202307.0430.v1

Optimizing Treatment for Relapsed/Refractory Classic Hodgkin lymphoma in the Era of Immunotherapy

Michael P. Randall, Michael A. Spinner

Subject: Medicine And Pharmacology, Hematology Keywords: Hodgkin lymphoma; relapsed/refractory; brentuximab vedotin; checkpoint inhibitor; PD-1 inhibitor; nivolumab; pembrolizumab; autologous hematopoietic cell transplantation

Online: 6 July 2023 (11:44:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202208.0193.v1

Association between Liver Function Indicators, Immunoglobulins, and Toll-like Receptors and Neuropilin-1 Expression in Patients with COVID-19

Salma A. El Kazafy, Amr E. Ahmed, Yasser M. Fouad, Azza F. Said, Tarek M. Ali, Ahmad EI Askary, Hebatallah H. Assal, Osama M. Ahmed

Subject: Medicine And Pharmacology, Hematology Keywords: liver; Neuropilin-1; Toll-like receptors; COVID-19

Online: 10 August 2022 (05:05:04 CEST)

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Purpose: The study aimed to investigate if there were any links between liver function biomarkers and immunoglobulins levels in serum, and Toll-like receptors (TLRs) and neuropilin-1 (NRP1) in COVID-19 patients. The study also aimed to assess the accuracy—sensitivity, specificity, and area under the curve (AUC) by the receiver operator curve (ROC) analysis for immunoglobulins levels and TLRs expressions. Patients and Methods: This study included 150 patients (100 patients with confirmed COVID-19 and 50 healthy volunteers as a control group). Patients with COVID-19 were subdivided into two groups according to the severity of symptoms (moderate and severe, with 50 patients each). Serum C-reactive protein (CRP), alanine aminotransaminase (ALT), aspartate aminotransaminase (AST), albumin, lactate dehydrogenase (LDH), immunoglobulin (Ig) G, and IgM levels were estimated. TLRs (TLR2 and TLR4) and NRP1 gene expression in blood samples were investigated using quantitative real-time polymerase chain reaction (qRT-PCR). ROC analysis was also applied to determine the accuracy of various detected parameters in predicting the possibility of COVID-19 infection. Results: In COVID-19 patients, serum parameters related to liver function, except serum albumin, CRP, IgG, IgM, and TLR2, TLR4, and NRP1 mRNA expression levels, significantly elevated compared to controls. Severe COVID-19 patients exhibited significantly higher liver enzymes (ALT, AST and LDH), CRP, and TLR2 mRNA expression levels and lower albumin levels than the moderate group. In the moderate and severe groups, ALT, CRP, TLR2, and TLR4 had a significant positive correlation with IgM levels. ALT, AST, LDH, CRP, TLR2, and TLR4 showed a significant positive correlation with IgG levels in both groups. In both the moderate and severe groups, NRP1 expression was found to be significantly correlated with CRP, IgG, IgM, TLR2, and TLR4. In contrast, serum albumin levels exhibited a significant negative correlation with IgG and IgM levels only in the severe group, but they showed a significant negative correlation with TLR2, TLR4, and NRP1 expression in both moderate and severe groups. Serum ALT and AST activities were positively correlated with NRP1 expression in the moderate group but not in the severe group and as well as TLR2 and TLR4 expression in both the moderate and severe groups. ROC analysis indicated that AUC was higher than 0.800 for serum IgM level and TLR4 gene expression in moderate COVID-19 group. Conclusions: The increased liver function biomarkers in serum and NRP1 expression are closely correlated with sustained activations in humoral and innate immune responses during COVID-19 infection. As a result, TLR2, TLR4, and NRP1 could be targets for limiting COVID-19 infection and impairment effects on liver function. Moreover, detection of IgM level in serum and TLR4 expression in blood have a good accuracy in predicting the possibility of infection with COVID-19 in moderate cases.

Preprint ARTICLE | doi:10.20944/preprints202206.0230.v1

How the First Year of the COVID-19 Pandemic Impacted Patients’ Care in the Vascular Surgery Divisions of the Southern Regions of the Italian Peninsula

Eugenio Martelli, Giovanni Sotgiu, Laura Saderi, Massimo Federici, Giuseppe Sangiorgi, Matilde Zamboni, Allegra Rosa Martelli, Giancarlo Accarino, Giuseppe Bianco, Francesco Bonanno, Umberto Marcello Bracale, Enrico Cappello, Giovanni Cioffi, Giovanni Colacchio, Adolfo Crinisio, Salvatore De Vivo, Carlo Patrizio Dionisi, Loris Flora, Giovanni Impedovo, Francesco Intrieri, Luca Iorio, Gabriele Maritati, Piero Modugno, Mario Monaco, Giuseppe Natalicchio, Vincenzo Palazzo, Fernando Petrosino, Francesco Pompeo, Raffaele Pulli, Davide Razzano, Maurizio Ruggero Ruggieri, Carlo Ruotolo, Paolo Sangiuolo, Gennaro Vigliotti, Pietro Volpe, Antonella Biello, Pietro Boggia, Michelangelo Boschetti, Enrico Maria Centritto, Flavia Condò, Lucia Cucciolillo, Amodio Salvatore D'Amodio, Mario De Laurentis, Claudio Desantis, Daniela Di Lella, Giovanni Di Nardo, Angelo Disabato, Ilaria Ficarelli, Angelo Gasparre, Antonio Nicola Giordano, Alessandro Luongo, Mafalda Massara, Vincenzo Molinari, Andrea Padricelli, Marco Panagrosso, Anna Petrone, Serena Pisanello, Roberto Prunella, Michele Tedesco, Alberto Maria Settembrini

Subject: Medicine And Pharmacology, Hematology Keywords: COVID-19; carotid stenosis; abdominal aortic aneurysm; chronic limb-threatening ischemia; amputation; deep venous thrombosis

Online: 16 June 2022 (04:40:54 CEST)

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Preprint REVIEW | doi:10.20944/preprints202206.0424.v1

COVID-19 Associated Thrombotic Thrombocytopenic Purpura: A Case Report and Systematic Review

Haseeb Chaudhary, Usama Nasir, Khezar Syed, Maria Labra, Christopher Reggio, Ansar Aziz, Parin Shah, Roopkia Reddy, Navdeep Sangha

Subject: Medicine And Pharmacology, Hematology Keywords: SARS-COV-2; COVID-19; TTP; refractory; thrombotic microangiopathies

Online: 30 June 2022 (09:06:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202212.0152.v1

Eugenio Martelli, Matilde Zamboni, Giovanni Sotgiu, Laura Saderi, Massimo Federici, Giuseppe Massimo Sangiorgi, Mariangela Valentina Puci, Allegra Rosa Martelli, Teresa Messina, Paolo Frigatti, Maria Pia Borrelli, Carlo Ruotolo, Ilaria Ficarelli, Paolo Rubino, Francesco Pezzo, Luciano Carbonari, Andrea Angelini, Edoardo Galeazzi, Luca Calia Di Pinto, Franco Michelino Fiore, Armado Palmieri, Giorgio Ventoruzzo, Giulia Mazzitelli, Franco Ragni, Antonio Bozzani, Enzo Forliti, Claudio Castagno, Pietro Volpe, Mafalda Massara, Diego Moniaci, Elisa Pagliasso, Tania Peretti, Mauro Ferrari, Nicola Troisi, Pietro Modugno, Maurizio Maiorano, Umberto Marcello Bracale, Marco Panagrosso, Mario Monaco, Giovanni Giordano, Giuseppe Natalicchio, Antonella Biello, Giovanni Maria Celoria, Alessio Amico, Mauro Di Bartolo, Massimiliano Martelli, Roberta Munaó, Davide Razzano, Giovanni Colacchio, Francesco Bussetti, Gaetano Lanza, Antonio Cardini, Bartolomeo Di Benedetto, Mario De Laurentis, Maurizio Taurino, Pasqualino Sirignano, Pierluigi Cappiello, Andrea Esposito, Santi Trimarchi, Silvia Romagnoli, Andrea Padricelli, Giorgio Giudice, Adolfo Crinisio, Giovanni Di Nardo, Giuseppe Battaglia, Rosario Tringale, Salvatore De Vivo, Rita Compagna, Valerio Stefano Tolva, Ilenia D'Alessio, Ruggiero Curci, Simona Giovannetti, Giuseppe D'Arrigo, Giusi Basile, Dalmazio Frigerio, Gian Franco Veraldi, Luca Mezzetto, Arnaldo Ippoliti, Fabio Massimo Oddi, Alberto Maria Settembrini

Subject: Medicine And Pharmacology, Hematology Keywords: chronic limb-threatening ischemia; outcome; sex; age; limb salvage

Online: 8 December 2022 (09:39:30 CET)

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Preprint REVIEW | doi:10.20944/preprints202304.0843.v2

Zanubrutinib: Coming of Age for BTK Inhibitors in the Treatment of Chronic Lymphocytic Leukemia?

Stefano Molica, Constantine Tam, David Allsup, Aaron Polliack

Subject: Medicine And Pharmacology, Hematology Keywords: CLL, Zanubrutinib, BTK inhibitors, efficacy, safety, chronic lymphocytic leukemia

Online: 22 May 2023 (16:15:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202111.0578.v1

Diphenyleneiodonium Triggers Cell Death of Acute Myeloid Leukaemia Cells by Blocking the Mitochondrial Respiratory Chain and Synergizes with Cytarabine

Hassan Dakik, Maya El Dor, Jérôme Bourgeais, Farah Kouzi, Olivier Herault, Fabrice Gouilleux, Kazem Zibara, Frédéric Mazurier

Subject: Medicine And Pharmacology, Hematology Keywords: DPI; Mitochondria; Leukaemia; Oxidative stress; OxPhos; Ara-C

Online: 30 November 2021 (21:37:18 CET)

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Preprint ARTICLE | doi:10.20944/preprints202302.0469.v1

Evaluation of the Haematological Patterns from up to 985 Days of Long COVID: A Cross-Sectional Study

Vanessa Costa Alves Galúcio, Daniel Carvalho de Menezes, Patrícia Danielle Lima de Lima, Vera Regina da Cunha Menezes Palácios, Pedro Fernando da Costa Vasconcelos, Juarez Antônio Simões Quaresma, Luiz Fábio Magno Falcão

Subject: Medicine And Pharmacology, Hematology Keywords: Long COVID; Laboratory Markers; Haematological Tests

Online: 27 February 2023 (09:53:40 CET)

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Preprint ARTICLE | doi:10.20944/preprints202306.0579.v1

Haematological Profile of Hb Adana Among High School Students in Northeast of Peninsular, Malaysia

Lee Miin Phoon, Mat Jusoh Siti Asmaa, Nur Atikah Zakaria, Suryati Hussin, Rosnah Bahar, Mohd Nazri Hassan, Zefarina Zulkafli, Salfarina Iberahim, Marne Abdullah, Noor Haslina Mohd Noor, Shafini Mohd Yusoff, Marini Ramli

Subject: Medicine And Pharmacology, Hematology Keywords: Hb Adana; non-deletional α-thalassaemia; Hb Constant Spring; HbE

Online: 8 June 2023 (03:58:49 CEST)

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Hb Adana is a non-deletional α-thalassaemia variant, due to mutations in α1- or α2-globin codon 59 (αCD59) that produce unstable α-globin. Clinical appearance can range from silent carrier to blood transfusion reliance, hepatosplenomegaly, skeletal deformities, and spinal cord compression. Despite the importance of Hb Adana inheritance, this Hb variant is challenging to study since molecular tests are scarce and routine diagnosis can be missed. This study explored the distribution of Hb Adana among local high school students and evaluate the hematological parameters and haemoglobin analysis of Hb Adana in this region of Malaysia. This retrospective study included 13,721 blood samples from high school students enrolled in Malaysia's National Thalassaemia Screening Program at Hospital Raja Perempuan Zainab II (HRPZ II). Multiplex Gap PCR was used to detect deletional α-thalassaemia. Common non-deletional α-thalassaemia was detected using multiplex ARMS PCR. Evaluable data were retrieved from the HRPZ II database. Results: A total of 2327 subjects exhibited common deletional (n=1037, 44.6%) and non-deletional (n=1290, 55.4%) α-thalassaemia. Hb Constant Spring was the most frequent non-deletional α-thalassaemia. Thirty-one participants (1.33%) had αCD59α/αα and one (0.04%) had αCD59α/-α3.7. The 32 Hb Adana subjects were 87.5% Malay, and 12.5% Orang Asli. Additionally, seven cases of HbE/Hb Adana were discovered. The haemoglobin level in heterozygous Hb Adana ranged from mild anaemia to normal, 95.0 g/L to 153.0 g/L. The MCV and MCH were approximately 73 fL and 23 pg, respectively. Conclusions: We have denoted the distribution of alpha thalassaemia mutation patterns among high school students in Kelantan, Northeast Peninsular of Malaysia. Our observation showed Hb Adana was rarely uncommon in our region and the co-inheritance with an α-gene deletion generates α+-thalassaemia; with HbE, α0-thalassaemia. All heterozygous Hb Adana exhibited low MCVs and MCHs.

Preprint REVIEW | doi:10.20944/preprints202304.1236.v1

Gilteritinib: The Story of a Proceeding Success into Hard-to-Treat FLT3 Mutated AML Patients

Matteo Molica, Salvatore Perrone, Marco Rossi

Subject: Medicine And Pharmacology, Hematology Keywords: FLT3 mutations; resistant/relapsed acute myeloid leukemia; tyrosine kinase inhibitors; gilteritinib

Online: 29 April 2023 (10:55:21 CEST)

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Preprint REVIEW | doi:10.20944/preprints202309.0848.v1

p53 Biology and Reactivation for Improved Therapy in MDS and AML–Are We Building Castles in the Air?

Joanna Zawacka

Subject: Medicine And Pharmacology, Hematology Keywords: MDS; AML; p53; MDM2; MDM4; p73; improved therapy

Online: 13 September 2023 (10:09:18 CEST)

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Preprint REVIEW | doi:10.20944/preprints202309.0378.v1

Recommendations for the Clinical Approach to Primary Immune Thrombocytopenia

Maria Eva Mingot-Castellano, Mariana Canaro Hirnyk, Blanca Sánchez-González, Maria Teresa Alvarez-Roman, Abelardo Bárez-García, Ángel Bernardo-Gutiérrez, Silvia Bernat-Pablo, Estefanía Bolaños-Calderón, Nora Butta-Coll, Gonzalo Caballero-Navarro, Isabel Socorro Caparrós-Miranda, Laura Entrena Ureña, Luis Fernando Fernández-Fuertes, Luis Javier García-Frade, María del Carmen Gómez del Castillo Solano, Tomás José González-López, Carlos Grande-García, José María Guinea de Castro, Isidro Jarque-Ramos, Reyes Jiménez-Bárcenas, Elsa López-Ansoar, Daniel Martínez-Carballeira, Violeta Martínez-Robles, Emilio Monteagudo-Montesinos, José Antonio Páramo-Fernández, María del Mar Perera-Álvarez, Inmaculada Soto-Ortega, David Valcárcel-Ferreiras, Cristina Pascual-Izquierdo

Subject: Medicine And Pharmacology, Hematology Keywords: Primary immune thrombocytopenia; Glucocorticoids; Intravenous immunoglobulins; Fostamatinib; Rituximab; Recommendations

Online: 6 September 2023 (09:38:18 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202112.0207.v2

Hematological and Biochemical Reference Ranges for Population With Sickle Cell Disease at Steady-State in Tanzania

Anna Daniel Fome, Raphael Zozimus Sangeda, Emmanuel Balandya, Josephine Mgaya, Deogratius Soka, Furahini Tluway, Upendo Masamu, Siana Nkya, Julie Makani, Bruno P. Mmbando

Subject: Medicine And Pharmacology, Hematology Keywords: Hematological parameters; biochemical parameters; reference ranges; sickle cell disease; Tanzania; steady-state.

Online: 11 January 2022 (12:54:23 CET)

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Preprint ARTICLE | doi:10.20944/preprints202212.0368.v1

A Synergistic pH-Responsive serum albumin-based drug delivery system loaded with doxorubicin and pentacyclic triterpene betulinic acid for potential treatment of NSCLC

Zally Torres-Martinez, Daraishka Pérez, Grace Torres, Sthephanie Estrada, Clarissa Correa, Natasha Mederos, Kimberly Velazquez, Betzaida Castillo, Kai Griebenow, Yamixa Delgado

Subject: Medicine And Pharmacology, Hematology Keywords: Betulinic acid; bovine serum albumin; doxorubicin; drug delivery system; lung cancer; synergistic effect.

Online: 20 December 2022 (10:40:12 CET)

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Nanosized drug delivery systems (DDS) have been studied as a novel strategy against cancer due to their potential to simultaneously decrease drug inactivation and systemic toxicity and increase passive and/or active drug accumulation within the tumor(s). Triterpenes are plant-derived compounds with interesting therapeutic properties. Betulinic acid (BeA) is a pentacyclic triterpene which has great cytotoxic activity against different cancer types. Herein, we developed a nanosized protein-based DDS of bovine serum albumin (BSA) as the drug carrier combining two compounds: doxorubicin (Dox) and the triterpene BeA using an oil-water-like micro-emulsion method. Spectrophotometric assays were performed to determine protein and drug concentrations in the DDS. The biophysical properties of these DDS were characterized using dynamic light scattering (DLS) and circular dichroism (CD) spectroscopy confirming nanoparticle (NP) formation and drug loading into the protein structure, respectively. The encapsulation efficiency was 77% for Dox and 18% for BeA. More than 50% of both drugs were released within 24 h, at pH 6.8, while less drug was released at pH 7.4 in this time period. Co-incubation viability assays of Dox and BeA alone for 24 h demonstrated synergistic cytotoxic activity in the low μM range against the non-small cell lung carcinoma (NSCLC) A549 cells. Viability assays of the BSA(Dox+BeA) DDS demonstrated a higher synergistic cytotoxic activity than the two drugs with no carrier. Moreover, confocal microscopy analysis confirmed cellular internalization of the DDS and nucleus accumulation of the Dox. We determined the mechanism of action of the BSA(Dox+BeA) DDS, confirming S-phase cell cycle arrest, DNA damage, caspase cascade activation, and downregulation of the epidermal growth factor receptor (EGFR) expression. This DDS has the potential to synergistically maximize the therapeutic effect of Dox and diminish chemoresistance induced by EGFR expression using a natural triterpene against NSCLC.

Preprint ARTICLE | doi:10.20944/preprints202303.0415.v1

Biblical Perspectives as a Guide to Research on Life’s Origin and History

Hugh Ross

Subject: Medicine And Pharmacology, Hematology Keywords: spacetime theorems; naturalism; abiogenesis; panspermia; process structuralism; Cambrian explosion; amino acids; homochirality; hand of God dilemma

Online: 23 March 2023 (13:40:22 CET)

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Preprint ARTICLE | doi:10.20944/preprints202111.0320.v1

Elastic Laminar Reorganization Occurs with Outward Diameter Expansion during Collateral Artery Growth and Requires Lysyl Oxidase for Stabilization

Ryan M McEnaney, Dylan D McCreary, Nolan Skirtich, Elizabeth Andraska, Ulka Sachdev, Edith Tzeng

Subject: Medicine And Pharmacology, Hematology Keywords: Arteriogenesis; Arterial structure; extracellular matrix; peripheral arterial disease; collateral circulation

Online: 18 November 2021 (10:59:12 CET)

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Preprint ARTICLE | doi:10.20944/preprints202302.0311.v1

Morphology and Hemodynamics of Cerebral Arteries and Aneurysms in a Pair of Monozygotic Twins

Hang Yi, Zifeng Yang, Luke C Bramlage, Ludwig R Bryan

Subject: Medicine And Pharmacology, Hematology Keywords: Monozygotic twins (MTs); morphology; hemodynamics; environmental and genetic factors; neurovascular diseases; cerebral aneurysms (CAs)

Online: 17 February 2023 (13:01:18 CET)

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The contribution of genetic and environmental factors to the pathophysiology of cerebral aneurysms in monozygotic twins is under-reported and presents ambiguous arguments. The morphology and hemodynamics of neurovascular arteries in a pair of monozygotic twins (MTs) were investigated to reveal the underlying mechanisms. Four arterial models were reconstructed for the twin A-right brain and left brain, twin B-left brain, and B-left brain without anterior cerebral arteries based on preclinical scanned information. Subsequently, the dimensions, configurations and outlined curves of the three-perspective geometries were compared between the MTs. Adopting an in-vitro validated numerical cerebral aneurysm model, hemodynamic patterns were investigated and compared in the MT models, respectively. Morphological comparisons of the MTs show the size and shape of cerebral arteries exist significant differences, despite of the expected genetic similarities. These differences can be attributed to variations during embryological development and external environmental influences. Qualitatively and generally, numerical results indicate the MTs have some hemodynamic similarities (e.g., time-averaged pressure (TAP) distributions (~13400 Pa), and oscillatory shear index (0~0.49), but present significant differences in specific local arteries due to morphological variances. Specifically, the difference in the volumetric blood flow rate in corresponding arteries between the MTs is from 16% (smallest) in anterior choroidal artery (AChA) to 221% (largest) in the ophthalmic artery (OphA), varying with specific compared arteries. Also, the registered hemodynamic indicators, such as the maximum time-averaged wall shear stress (TWSS) (53.6 Pa vs. 37.8 Pa), and different local OSI distributions were observed between the MTs. The findings revealed that morphological variations in MTs could be generated by embryological and environmental factors, thus assuming they share the identical morphology in cardiovascular and neurovascular systems may lead to significant misevaluations in hemodynamics quantifications and further lesions.

Preprint REVIEW | doi:10.20944/preprints202201.0208.v1

Advance Directives in Oncology and Haematology: A Long Way to Go. A Narrative Review

Kevin Serey, Amélie Cambriel, Adrien Pollina-Bachellerie, Jean-Pierre Lotz, François Philippart

Subject: Medicine And Pharmacology, Hematology Keywords: End of Life; Advance Directives; Advance Care Planning; Intensive Care, Medical Oncology; malignant hemopathy

Online: 14 January 2022 (11:34:51 CET)

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Preprint ARTICLE | doi:10.20944/preprints202307.1234.v1

Comparison of Efficiency and Side Effects of Daily and Alternate Day Oral Iron

Ibrahim Nahit Sendur, Umit Yavuz Malkan

Subject: Medicine And Pharmacology, Hematology Keywords: Iron deficiency anemia; oral iron; side effects

Online: 18 July 2023 (14:17:18 CEST)

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Introduction: In the recent years alternate day dosing has been recommended in iron deficieny treatment. The aim of this study is to compare the efficacy and side effects of oral iron treatments given daily 1x1, alternate day 1x1 and 2x1 alternate day dosing in iron deficieny anemia patients. Methods: A total of 52 patients with a diagnosis of iron deficiency anemia with serum ferritin level of 25 µg/L or less were included in this retrospective study. Statistical analyzes were done with SPSS V.25. Results: The patients included in our study were between the ages of 18-51. All of the patients included in the study were female. A statistically significant increase was found between 0-14 days(p<0.001), 14-28 days(p<0.001) and 0-28 days(p<0.001) hemoglobin values in all patients. There was no statistically significant difference between 0-14 days (p=0.397) and 0-28 days(p=0.310) ferritin values in all patients. A statistically significant difference was found between 14-28 days(p<0.001) ferritin values. When the rate of change of hemoglobin and ferritin values between the groups was compared and no statistically significant difference was observed between the groups. The rate of change of ferritin values for 14-28 days was significant (p=0.012). There was no significant difference in the frequency of symptoms in the 14th day and 28th day controls for each group (p>0.05). The incidence of metallic taste and bloating symptoms was found to be statistically significant in the third control in the group with 2x1 drug use alternate day (p=0.094). Discussion: The only difference in efficacy was observed in ferritin values between 14th and 28th days. The increase in ferritin values was higher in the group that used 1x1 oral iron every day. However, since no difference was observed between the groups in terms of ferritin and hemoglobin values on days 0 and 28, all three groups were considered equally efficient. Side effects were mainly gastrointestinal side effects. Since the group that received 2x1 oral treatment alternate day had a statistically significantly higher rate of side effects, the patients’ treatment tolerance will be lower compared to the other groups. In conlusion, there is no difference in efficacy and side effects between the patient groups receiving 1x1 daily and 1x1 alternate day oral iron therapy, so 1x1 use alternate day is the most appropriate treatment method for oral iron therapy in terms of patient tolerance, adherence to treatment and pharmacoeconomics.

Preprint REVIEW | doi:10.20944/preprints202401.0262.v1

p38 MAPK Molecular Targeting for Next-Generation Multiple Myeloma Therapy

Mario Morales-Martinez, Mario I Vega

Subject: Medicine And Pharmacology, Hematology Keywords: multiple myeloma; p38 mapk; transcriptional factor; cancer progression; clinical implication; gene expression

Online: 4 January 2024 (05:44:50 CET)

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Preprint ARTICLE | doi:10.20944/preprints202302.0320.v2

Evaluation of a Novel Methodology for Real-Time Calculation of Critical Blood Values Based on Four Measured Values

Rüdiger H. Ettrich, Joshua Caballero, Sultan Ahmed, Traci Hurlston, Jayesh Parmar, Subrata Deb

Subject: Medicine And Pharmacology, Hematology Keywords: non-invasive; point-of-care testing; blood values; Radial arterial blood; blood gas; transcutaneous; algorithm

Online: 4 May 2023 (03:07:48 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.0213.v1

Understanding the Intricacies of Iron Overload Associated With β-Thalassemia: A Comprehensive Review

Subhangi Basu, Motiur Rahaman, Tuphan Kanti Dolai, Praphulla Chandra Shukla, Nishant Chakravorty

Subject: Medicine And Pharmacology, Hematology Keywords: β-thalassemia; iron overload; iron chelators; TDT; NTDT

Online: 2 June 2023 (14:27:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202111.0438.v1

Superb Microvascular Imaging as a Novel Tool for the Assessment of Blood Flow Velocity in Patients with Systemic Sclerosis: A Single-Center Feasibility Study

Jan-Gerd Rademacher, Rosa Marie Buschfort, Thomas Asendorf, Viktor Korendovych, Björn Tampe, Peter Korsten

Subject: Medicine And Pharmacology, Hematology Keywords: Systemic Sclerosis; Raynaud’s phenomenon; ultrasound; microvascular imaging; blood flow

Online: 23 November 2021 (16:15:51 CET)

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Preprint REVIEW | doi:10.20944/preprints202402.1767.v1

Identification and Diagnosis of Complete Haptoglobin Gene Deletion, the Gene Responsible for Adverse Posttransfusion Reactions

Mikiko Soejima, Yoshiro Koda

Subject: Medicine And Pharmacology, Hematology Keywords: adverse reactions, haptoglobin deficiency, haptoglobin gene deletion, serum protein, transfusion,.

Online: 29 February 2024 (21:07:24 CET)

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Preprint ARTICLE | doi:10.20944/preprints202308.1141.v1

Prevalence and Significance of Elevated Cardiac Enzymes among Sickle Cell Disease Patients at King Abdulaziz University Hospital

Roaa Shaban, Osman O. Radhwi, Nadin Alharbi, Sondos Jar, Weam Bajunaid, Abdulaziz Alzahrani, Majed Alhuzali, Adel F. Almarzouki

Subject: Medicine And Pharmacology, Hematology Keywords: sickle cell; cardiac markers; vaso-occlusive; troponin; lactate dehydrogenase

Online: 16 August 2023 (03:18:46 CEST)

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Preprint CASE REPORT | doi:10.20944/preprints202308.1585.v1

Manifestation of Pancytopenia Associated with COVID-19 as Paroxysmal Nocturnal Hemoglobinuria (PNH) and Aplastic Anemia (AA)

Jeff Justin Aguilar, Vikram Dhillon, Suresh Balasubramanian

Subject: Medicine And Pharmacology, Hematology Keywords: Paroxysmal nocturnal hemoglobinuria; aplastic anemia; SARS-CoV-2

Online: 22 August 2023 (13:19:19 CEST)

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Preprint REVIEW | doi:10.20944/preprints202312.1578.v1

Vaccine-Induced Immune Thrombotic Thrombocytopenia—Clinicopathologic Features and New Perspectives on Anti-PF4 Antibody-Mediated Disorders

Yi Zhang, Anna-Lise Bissola, Jared Treverton, Michael Hack, Mark Lychacz, Sarah Kwok, Addi Arnold, Ishac Nazy

Subject: Medicine And Pharmacology, Hematology Keywords: vaccine-induced immune thrombotic thrombocytopenia; adenoviral-vector based COVID-19 vaccines; platelet-factor 4; anti-PF4 antibody-mediated disorders; platelet; SARS-CoV-2

Online: 20 December 2023 (16:45:08 CET)

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Preprint REVIEW | doi:10.20944/preprints202305.1725.v1

Molecular Insights into the Role of Gut Microbiota in Antibiotic Therapy Selection and Resistance Mitigation

Mihaela Andreescu

Subject: Medicine And Pharmacology, Hematology Keywords: Molecular biology; infectious diseases; clinical diagnostic; early detection; prognosis

Online: 25 May 2023 (03:34:18 CEST)

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Preprint REVIEW | doi:10.20944/preprints202401.0994.v1

Approach to Acute Myeloid Leukemia with Increased Eosinophils and Basophils

Stavros Papadakis, Ioannis Liapis, Stefanos I. Papadhimitriou, Emmanouil Spanoudakis, Ioannis Kotsianidis, Konstantinos Liapis

Subject: Medicine And Pharmacology, Hematology Keywords: acute myeloid leukemia; AML; eosinophil; eosinophilia; basophil; basophilia; morphology; CBF; PDGFRA; PDGFRB

Online: 12 January 2024 (07:13:16 CET)

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Preprint REVIEW | doi:10.20944/preprints202304.1161.v1

Belantamab Mafodotin: From Clinical Trials Data to Real Life Experiences

Sonia Morè, Massimo Offidani, Laura Corvatta, Maria Teresa Petrucci, Francesca Fazio

Subject: Medicine And Pharmacology, Hematology Keywords: Multiple myeloma; belantamab mafodotin; antibody-drug-conjugate.

Online: 28 April 2023 (10:12:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1500.v1

Autologous Transplantation May Still Effectively Treat Relapsed Diffuse Large B-cell Lymphoma in Selected Patients

Reka Rahel Bicsko, Lili Antal, Ferenc Magyari, Róbert Szász, Miklós Udvardy, Arpad Illes, Lajos Gergely

Subject: Medicine And Pharmacology, Hematology Keywords: diffuse large B-cell lymphoma; relapsed; refractory; autologous stem cell transplantation

Online: 22 May 2023 (10:29:20 CEST)

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Preprint REVIEW | doi:10.20944/preprints202403.0836.v1

Precision Medicine Approaches in Acute Myeloid Leukemia with Adverse Genetics

Nicole Santoro, Prassede Salutari, Mauro Di Ianni, Andrea Marra

Subject: Medicine And Pharmacology, Hematology Keywords: acute myeloid leukemi; adverse genetics; leukemogenesis; targeted therapy; immunotherapy

Online: 14 March 2024 (10:08:08 CET)

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Preprint ARTICLE | doi:10.20944/preprints202210.0002.v1

Therapeutic Approach of Anticoagulants and Acute Blood Purification for Patients with Sepsis-Induced Disseminated Intravascular Coagulation: Post-marketing Surveillance Data of Antithrombin Supplementation

Yutaka Eguchi, Hiroyuki Nagafuchi, Toshiaki Ikeda

Subject: Medicine And Pharmacology, Hematology Keywords: antithrombin; recombinant thrombomodulin; SOFA score; JAAM DIC score; PMX-DHP

Online: 1 October 2022 (07:09:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202212.0087.v1

Interrelation Midst Low T3 Levels, Type 3 Deiodinase, Oxidative Stress And Mortality In Sepsis and Septic Shock: Defining Patient Outcome

Josi Vidart, Luiza Axelrud, Andre Cardozo Braun, Rafael A. Marschner, Simone Magagnin Wajner

Subject: Medicine And Pharmacology, Hematology Keywords: low T3 syndrome, oxidative stress, sepsis, septic shock, thyroid hormone, type 3 deiodinase

Online: 6 December 2022 (02:18:24 CET)

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Preprint REVIEW | doi:10.20944/preprints202308.0936.v1

Early Complications in the Era of ATRA Plus ATO Therapy Outside of Clinical Trials‐One Center Experience during Three Years and Literature Review‐

Ghiaur Alexandra, Cristina Doran, Bogdan Ionescu, Maria Camelia Stancioaica, Roxana Hirjan, Aurelia Tatic, Mihaela Cirstea, Mihaela Dragomir, Onda Calugaru, Codruta Popa, Cerasela Jardan, Didona Vasilache, Daniel Coriu

Subject: Medicine And Pharmacology, Hematology Keywords: acute promyelocytic leukemia; arsenic trioxide; all‐trans retinoic acid; non‐hematologic toxicities

Online: 14 August 2023 (09:52:50 CEST)

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Abstract The hallmark of acute promyelocytic leukemia (APL) is the presence of the characteristic fusion transcript of the promyelocytic leukemia gene with the retinoic acid receptor α gene (PML::RARA), which is a molecular target for all-transretinoic acid (ATRA) and arsenic trioxide (ATO). Therapies with ATRA plus ATO have excellent outcomes in terms of complete remission rate, overall survival, and achievement of a deep and durable molecular response with a very low incidence of relapse. However, although the combination of ATRA and ATO has lower hematologic toxicity than standard chemotherapy, its use is associated with a spectrum of unique toxicities, such as hepatic toxicity, QTc (QT interval corrected) prolongation, neurotoxicity, and differentiation syndrome. Hematologists treating patients with acute promyelocytic leukemia should be aware of possible early-complications of the ATRA plus ATO regimen and aim to balance treatment-related adverse events and efficacy. Careful follow-up of the patient’s clinical course is essential to identify and treat each complication. This study focused on treatment-related complications during induction and consolidations in patients with APL who received ATRA plus ATO. We retrospectively analyzed 26 adult patients diagnosed with APL between 2019 and 2022 at our center to identify the incidence rate, severity and time of onset of short-term non-hematologic adverse events relative to the initiation of the ATRA and ATO regimen. Additionally, we review the existing literature regarding the toxic side effects of ATO in APL and discuss how its most frequent toxicities should be managed. The median follow-up of our patients was 21.5 months (range, 1-48 months) and the most common adverse events were hepatic toxicity (69.2%), infections (46.1%), differentiation syndrome(23%) and QT prolongation (11.5%). All patients experienced at least one adverse event and grade 3 and 4 toxicities occurred in 57.6 % patients; these generally consisted of liver enzyme elevations, QTc prolongation, but also some rare manifestations such as musculoskeletal pain syndrome (1 patient) and ATO-related pancreatitis (1 patient). Most patients developed side-effects during induction. This is the first study about Romanian patients diagnosed with APL. Our results suggest that all treatment-related adverse events, except for differentiation syndrome are manageable, self-limiting and reversible. All patients, except for two (one died during induction and one died during consolidation), were able to complete the treatment consolidation with ATO and ATRA.

Preprint REVIEW | doi:10.20944/preprints202307.1943.v1

Developing New Strategies for Relapsed/Refractory Diffuse Large B-Cell Lymphoma

EVA GONZALEZ-BARCA

Subject: Medicine And Pharmacology, Hematology Keywords: diffuse large B-cell lymphoma. relapsed/refractory; therapy; efficacy; toxicity

Online: 28 July 2023 (03:55:02 CEST)

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Preprint REVIEW | doi:10.20944/preprints202309.0229.v1

Immunotherapy of Acute Myeloid Leukemia: A Work in Progress

Matteo Molica, Salvatore Perrone, Marco Rossi

Subject: Medicine And Pharmacology, Hematology Keywords: acute myeloid leukemia; immunotherapy; checkpoint inhibitors; new therapeutic landscapes

Online: 6 September 2023 (14:36:13 CEST)

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Preprint REVIEW | doi:10.20944/preprints202303.0371.v1

Nuclei on the Rise: When Nuclei-Based Methods Meet Next-Generation Sequencing

Tamer Butto, Kanak Mungikar, Peter Baumann, Jennifer Winter, Beat Lutz, Susanne Gerber

Subject: Medicine And Pharmacology, Hematology Keywords: Nuclei isolation; Next-generation sequencing; Cell-type specific isolation; Epigenetics; Transcriptomics; Single cell sequencing; Single nucleus sequencing

Online: 21 March 2023 (06:40:11 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.0265.v1

Type II JAK2 Inhibitor CHZ‐868 and HSP90 Inhibitors Are Potent towards the Ruxolitinib Resistant JAK2 Variants in JAK2 Driven Myeloproliferative Neoplasms

Sivahari Prasad Gorantla, Gerin Prince, Jasmin Osius, Dhurvas Dinesh, Vijay Kumar Boddu, Justus Duyster, Nikolas Von Bubnoff

Subject: Medicine And Pharmacology, Hematology Keywords: Myeloproliferative neoplasms; JAK2-V617F mediated resistance; ruxolitinib; JAK2-STAT5 signaling; HSP90 inhibition

Online: 3 January 2024 (14:39:52 CET)

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Preprint ARTICLE | doi:10.20944/preprints202305.0700.v1

Autoimmune Limbic Encephalitis in Patients With Hematologic Malignancies After Haploidentical Hematopoietic Stem Cell Transplantation With Post-transplant Cyclophosphamide

Bu Yeon Heo, Myung-Won Lee, Suyoung Choi, Yunju Jung, Thi Thuy Duong Pham, Yunseon Jang, Jung-Hyun Park, Sora Kang, Jeong Suk Koh, Deog-Yeon Jo, Jaeyul Kwon, Ik-Chan Song

Subject: Medicine And Pharmacology, Hematology Keywords: allogeneic hematopoietic stem cell transplantation; autoimmune limbic encephalitis; cyclo-phosphamide; regulatory T cells; CD25; Foxp3; IL-6; fever; acute graft-versus-host disease; cyto-kine-release syndrome

Online: 10 May 2023 (07:46:24 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.2138.v1

The Real-World Evidence on the Fragility and Its Impact on the Choice of Treatment Regimen in Newly Diagnosed Patients With Multiple Myeloma Over 75 Years of Age

Agata Tyczyńska, Marcela Krzysława Krzemek, Alexander Jorge Cortez, Artur Jurczyszyn, Katarzyna Godlewska, Hanna Ciepłuch, Edyta Subocz, Janusz Hałka, Anna Kulikowska de Nałęcz, Anna Wiśniewska, Alina Swiderska, Anna Waszczuk-Gajda, Joanna Drozd-Sokołowska, Renata Guzicka-Kazimierczak, Agnieszka Porowska, Kamil Wiśniewski, Wanda Knopińska-Posłuszny, Janusz Kłoczko, Piotr Rzepecki, Dariusz Woszczyk, Hanna Symonowicz, Grzegorz Władysław Basak, Barbara Zdziarska, Krzysztof Jamroziak, Jan M. Zaucha

Subject: Medicine And Pharmacology, Hematology Keywords: frailty; elderly; multiple myeloma; choice of treatment; over 75 years old; early mortality

Online: 30 May 2023 (12:26:24 CEST)

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Preprint REVIEW | doi:10.20944/preprints202310.0556.v1

Leukemia Cutis—the Current View on Pathogenesis, Diagnosis and Treatment

Ewa Robak, Marcin Braun, Tadeusz Robak

Subject: Medicine And Pharmacology, Hematology Keywords: acute leukemia; chronic leukemia; cutis; diagnosis; pathogenesis; prolympchocytic leukemia; Richter transformation; skin lesions; treatment

Online: 10 October 2023 (05:36:42 CEST)

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Preprint REVIEW | doi:10.20944/preprints202307.1039.v1

A Review of the Relationship between the Immune Response, Inflammation, Oxidative Stress, and the Pathogenesis of Sickle cell Anaemia

Florence Ifechukwude Aboderin, Taofeeq Oduola, Glenda Mary Davison, Oluwafemi Omoniyi Oguntibeju

Subject: Medicine And Pharmacology, Hematology Keywords: Keywords: sickle cell anaemia, chronic inflammation, immune system, oxidative stress, hemolysis, blood transfusion

Online: 17 July 2023 (09:25:33 CEST)

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Preprint REVIEW | doi:10.20944/preprints202311.1158.v1

Parenteral Iron Therapy for Pediatric Patients

Elpis Mantadakis, Sonia Alexiadou, Panagiota Zikidou

Subject: Medicine And Pharmacology, Hematology Keywords: iron deficiency; iron deficiency anemia; intravenous iron; iron sucrose; ferric carboxymaltose; iron isomaltoside; inflammatory bowel diseases

Online: 20 November 2023 (02:41:19 CET)

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Preprint ARTICLE | doi:10.20944/preprints202306.1076.v1

Enhancing the Anti-Leukemic Potential of Thymoquinone- SBE-β-CD Inclusion Complex

ELTAYEB E.M. EID, Salah Abdalrazak Alshehade, Amer A. Almiman, Sareh Kamran, Vannajan Sanghiran Lee, Mohammed Abdullah Alshawsh

Subject: Medicine And Pharmacology, Hematology Keywords: Thymoquinone; SBE-β-CD; Leukemia; Drug delivery system; Telomerase inhibition; Apoptosis

Online: 15 June 2023 (05:37:50 CEST)

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Preprint REVIEW | doi:10.20944/preprints202401.0323.v1

Haemophilia and Cancer: A Systematic Review

Ezio Zanon, Annamaria Porreca, Paolo Simioni

Subject: Medicine And Pharmacology, Hematology Keywords: haematology; haemophilia; cancer; malignancy; congenital bleeding disorders

Online: 4 January 2024 (03:47:09 CET)

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Preprint ARTICLE | doi:10.20944/preprints202305.1477.v1

Study of the Symptoms’ Severity and Adherence to Therapy of Myelofibrosis Patients Treated With Ruxolitinib

Vera Stoeva, Guenka Petrova, Konstantin Mitov, Konstantin Tihomirov Tachkov

Subject: Medicine And Pharmacology, Hematology Keywords: myelofibrosis; ruxolitinib; severity of symptoms; adherence to therapy

Online: 22 May 2023 (08:52:13 CEST)

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Preprint REVIEW | doi:10.20944/preprints202309.0016.v1

Neutropenia in Childhood. A Narrative Review and Practical Diagnostic Approach

Georgios Katsaras, Silouani Koutsi, Evdokia Psaroulaki, Dimitra Gouni, Pelagia Tsitsani

Subject: Medicine And Pharmacology, Hematology Keywords: neutropenia; neutrophils; childhood

Online: 1 September 2023 (11:49:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.2148.v1

Real-World Clinical Characterization of Polycythemia Vera Patients From a Prospective Registry in Portugal: Is Resistance to Hydroxyurea a Reality?

Maria Sarmento, Marta Duarte, Sandra Ponte, Juan Sanchez, Diana Roriz, Laura Fernandes, Maria José Monteiro Silva, Judite Pacheco, Gisela Ferreira, Jorge Freitas, Inês Costa, Daniel Brás

Subject: Medicine And Pharmacology, Hematology Keywords: disease management; hemorragic events; hydroxyurea; resistance; symptomatic evaluation; patient-reported outcomes; thrombotic events; polycythemia vera

Online: 30 May 2023 (13:11:32 CEST)

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Preprint CASE REPORT | doi:10.20944/preprints202306.1128.v1

Myeloid Sarcoma as an Unusual Presentation of Acute and Chronic Myeloid Leukemia: A Case Series

Ugo Giordano, Mateusz Sawicki, Justyna Pilch, Jakub Mizera, Artur Borkowski, Krzysztof Zduniak, Jarosław Dybko

Subject: Medicine And Pharmacology, Hematology Keywords: myeloid sarcoma; acute myeloid leukemia; chronic myeloid leukemia; chloroma; granulocytic sarcoma; case report

Online: 16 June 2023 (07:15:03 CEST)

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Preprint REVIEW | doi:10.20944/preprints202307.1091.v1

Hematopoietic Stem Cell Transplantation in Acute Promyelocytic Leukemia in the ATRA/ATO Era

Andrei Colita, Alina Daniela Tanase, Ciprian Tomuleasa, Anca Colita

Subject: Medicine And Pharmacology, Hematology Keywords: acute promyelocytic leukemia; relapse; hematopoietic stem cell transplantation

Online: 17 July 2023 (10:10:56 CEST)

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Preprint REVIEW | doi:10.20944/preprints202403.0755.v1

Autoimmune Hemolytic Anemias

Melika Loriamini, Christine Cserti-Gazdewich, Donald R. Branch

Subject: Medicine And Pharmacology, Hematology Keywords: autoimmune hemolytic anemia; AIHA; categories; diagnosis; CAD; PCH

Online: 13 March 2024 (07:20:53 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.0728.v1

Cell‐Free DNA as a Biomarker at Diagnosis and Follow up in 256 B and T Cell Lymphomas

Ramón Diez-Feijoo, Marcio Andrade-Campos, Joan Gibert, Blanca Sánchez-González, Lierni Fernández-Ibarrondo, Concepción Fernández-Rodríguez, Nieves Garcia-Gisbert, Laura Camacho, Marta Lafuente, Ivonne Vázquez, Lluís Colomo, Antonio Salar, Beatriz Bellosillo

Subject: Medicine And Pharmacology, Hematology Keywords: cfDNA; Liquid biopsy; Lymphoma; monitoring

Online: 11 December 2023 (15:59:03 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.1332.v1

Application of Optical Genome Mapping to the Risk Stratification and Treatment Optimization of Hematologic Diseases

Meena Naik, Nishit Ojha, Trilochan Sahoo, Amey Panchal, Yesheswini Naik, Rahul Arora, Vishvdeep Khushoo, Vasu Babu, Nishad Dhakate, Poonam Jain, Punit Jain, Govind Babu, Jen Hauenstein, Anusha Myelavarapu, Beth Matthews, Stephen J Wicks, Alex Hastie, Alka Chaubey, Sandeep Rao, Sachin Jadhav

Subject: Medicine And Pharmacology, Hematology Keywords: structural variation; optical genome mapping; cytogenetic; hematological disorders

Online: 23 February 2024 (11:04:40 CET)

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Preprint REVIEW | doi:10.20944/preprints202308.1982.v1

The Role of Non-Coding RNAs in Myelodysplastic Neoplasms

Vasileios Georgoulis, Epameinondas Koumpis, Eleftheria Hatzimichael

Subject: Medicine And Pharmacology, Hematology Keywords: myelodysplastic syndromes; non-coding RNA; microRNA; lnc-RNA; circ-RNA; piwi-RNA; t-RNA; sno-RNA

Online: 29 August 2023 (13:42:14 CEST)

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Preprint REVIEW | doi:10.20944/preprints202309.2129.v1

Refractory Immune Thrombocytopenia or Myelodysplastic Syndrome Presenting with Isolated Thrombocytopenia? A Review of Literature and a Case Report

Riccardo Tomasello, Claudia Cammarata, Marta Mattana, Manuela Giuseppa Ingrascì, Giulio Giordano, Alessandro Lucchesi, Waleed Ghanima, Sergio Mario Siragusa, Mariasanta Napolitano

Subject: Medicine And Pharmacology, Hematology Keywords: Immune thrombocytopenia; chronic ITP; myelodysplastic syndrome; chronic myelomonocytic leukemia; refractory thrombocytopenia

Online: 30 September 2023 (07:14:13 CEST)

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Preprint CASE REPORT | doi:10.20944/preprints202308.1373.v1

Possible New Histological Prognostic Index for Large B-Cell Lymphoma

Hideaki Nitta, Haruko Takizawa, Toru Mitsumori, Hiroko Iizuka-Honma, Yoshihiko Araki, Maki Fujishiro, Shigeki Tomita, Satsuki Kishikawa, Akane Hashizume, Tomohiro Sawada, Mitsuo Okubo, Yasunobu Sekiguchi, Miki Ando, Masaaki Noguchi

Subject: Medicine And Pharmacology, Hematology Keywords: Large B-cell lymphoma (LBCL); Immunohistochemical (IHC) staining; Glucoseregulated protein 94 (GRP94); CYP3A4; Multidrug resistance protein 1 (MDR1); Aldo-keto reductase family 1 member C3 (AKR1C3); Multi-drug resistance-associated protein 1 (MRP1); P53

Online: 18 August 2023 (11:42:22 CEST)

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Preprint REVIEW | doi:10.20944/preprints202311.0714.v1

Primary Cutaneous CD30 positive Lymphoproliferative Disorders—Current Therapeutic Approach with Focus on Brentuximab Vedotin

Tomas Stein, Tadeusz Robak, Wojciech Biernat, Ewa Robak

Subject: Medicine And Pharmacology, Hematology Keywords: Anaplastic large cell lymphoma; Brentuximab vedotin; CD30 positive lymphomas; Diagnosis; Lymphomatoid papulosis; Primary cutaneous T-cell lymphoma; Treatment

Online: 13 November 2023 (08:27:17 CET)

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Preprint REVIEW | doi:10.20944/preprints202311.0006.v1

Engagement of Mesenchymal Stromal Cells in the Remodeling of Bone Marrow Microenvironment in Hematological Cancers

Sebastiano Giallongo, Andrea Duminuco, Ilaria Dulcamare, Tatiana Zuppelli, Enrico La Spina, Grazia Scandura, Annalisa Santisi, Alessandra Romano, Francesco Di Raimondo, Daniele Tibullo, Giuseppe Alberto Palumbo, Cesarina Giallongo

Subject: Medicine And Pharmacology, Hematology Keywords: MSCs; tumor transformation; hematological cancers; senescence; inflammation

Online: 1 November 2023 (04:05:53 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.1973.v1

Smart Conditioning with Venetoclax Enhanced Sequential FLAMSA + RIC in Patients with High-Risk Myeloid Malignancies

Felicitas Schulz, Paul Sebastian Jäger, Johanna Tischer, Alessia Fraccaroli, Gesine Bug, Andreas Hausmann, Ben Niklas Baermann, Patrick Tressin, Alexander Sebastian Hoelscher, Annika Kasprzak, Kathrin Nachtkamp, Johannes Schetelig, Inken Hilgendorf, Ulrich Germing, Sascha Dietrich, Guido Kobbe

Subject: Medicine And Pharmacology, Hematology Keywords: FLAMSA; allogeneic transplant (aHSCT); sequential conditioning; AML; MDS; myeloid malignancies; reduced-intensity conditioning; RIC; Venetoclax

Online: 30 November 2023 (10:24:31 CET)

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Preprint BRIEF REPORT | doi:10.20944/preprints202307.2162.v1

Long-Term L-Glutamine Treatment Reduces Hemolysis Without Ameliorating Hepatic Vasoocclusion and Liver Fibrosis in a Mouse Model of Sickle Cell Disease

Omika Katoch, Ramakrishna UV, Tomasz Kaminski, Ziming Li, Rikesh K Dubey, Isabella Burholt, Shweta Gudapati, Tirthadipa Pradhan-Sundd

Subject: Medicine And Pharmacology, Hematology Keywords: Sickle cell disease; hemolysis; vasoocclusion; l-glutamine; Liver injury; Kupffer cells

Online: 1 August 2023 (14:24:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.1450.v1

Thrombin Generation is Associated with Venous Thrombo-embolism Recurrence, but not with Major Bleeding and Death in the Elderly: A Prospective Multicenter Cohort Study

Kristina Vrotniakaite-Bajerciene, Sereina Rütsche, Sara Calzavarini, Claudia Quarroz, Odile Stalder, Marie Méan, Marc Righini, Daniel Staub, Juerg H. Beer, Beat Frauchiger, Joseph Österwalder, Nils Kucher, Christian M. Matter, Marc Husmann, Martin Banyai, Markus Aschwanden, Lucia Mazzolai, Olivier Hugli, Nicolas Rodondi, Drahomir Aujesky, Anne Angelillo-Scherrer

Subject: Medicine And Pharmacology, Hematology Keywords: Thrombin generation; venous thromboembolism; elderly; bleeding; mortality

Online: 21 July 2023 (12:46:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.0347.v1

The Role of Thromboelastography as a Predictor of Acute Kidney Injury in COVID-19 Patients in ICU

Usi Sukorini, Ira Puspitawati, Yulia Wardhani, Muhammad Roihan Munajih

Subject: Medicine And Pharmacology, Hematology Keywords: thromboelastography; acute kidney injury; COVID-19; hypercoagulable

Online: 6 March 2024 (13:07:01 CET)

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Preprint ARTICLE | doi:10.20944/preprints202305.0887.v1

ATO Increases ROS Production and Apoptosis of Cells by Enhancing Calpain-Mediated Degradation of the Cancer Survival Protein TG2

Károly Jambrovics, Szilárd Póliska, Beáta Scholtz, Iván P. Uray, Zoltán Balajthy

Subject: Medicine And Pharmacology, Hematology Keywords: transglutaminase 2; cancer; ATO

Online: 12 May 2023 (05:04:48 CEST)

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Preprint REVIEW | doi:10.20944/preprints202404.0091.v1

Menin Inhibitors: New Players in KMT2A-Rearranged and NPM1-Mutated Acute Myeloid Leukemia

Gabriele Coppola, Anna Candoni

Subject: Medicine And Pharmacology, Hematology Keywords: menin; menin inhibitors; leukemia; KMT2A; NPM1

Online: 2 April 2024 (02:51:43 CEST)

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Preprint REVIEW | doi:10.20944/preprints202312.0095.v1

Hematological Neoplasm with Eosinophilia

Rosario M Morales-Camacho, Teresa Caballero-Velázquez, Juan José Borrero, Ricardo Bernal, Concepción Prats-Martín

Subject: Medicine And Pharmacology, Hematology Keywords: Eosinophilia,; hematological neoplasm; myeloid/lymphoid neoplasm with eosinophilia; tyrosine kinase fusion genes; acute leukemia

Online: 1 December 2023 (16:34:12 CET)

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Preprint REVIEW | doi:10.20944/preprints202306.0849.v1

Epigenetic Alterations That Are the Backbone of Immune Evasion in T-Cell Malignancies

Mihaela Andreescu

Subject: Medicine And Pharmacology, Hematology Keywords: Molecular biology; infectious diseases; clinical diagnostic; early detection; prognosis

Online: 12 June 2023 (14:31:24 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.1391.v1

Prophylactic Therapy with Emicizumab in Patients with Hemophilia A: Benefits and Challenges

Melen Brinza, Valentina Uscatescu, Mihai Himcinschi, Elisabeta Chiriac, Georgiana Gherghe, Daniel Coriu

Subject: Medicine And Pharmacology, Hematology Keywords: hemophilia A; inhibitors; Emicizumab

Online: 21 September 2023 (03:32:12 CEST)

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This study presents a transversal investigation, that we performed at Fundeni hospital, into the therapeutic benefits and efficacy of Emicizumab, a non-factor therapy, in the context of hemophilia A. Ten patients diagnosed with hemophilia A were closely monitored, using clinical and laboratory resources, during the course of Emicizumab treatment, with an average of 12.8 months. Among these patients, six exhibited anti-factor VIII inhibitors, changing the medical approach and adding complexity to their clinical profiles. A comprehensive approach was adopted to assess the coagulation status of patients under Emicizumab therapy. The study employed several key coagulation monitoring tools, of which including thrombin generation time (TGT) and thrombelastography (TEG). These methodologies proved highly valuable results in evaluating the patients' coagulation profiles during the treatment regimen. Additionally, traditionally coagulation assays were utilized to gain a comprehensive understanding of the overall coagulation dynamics. Noteworthy, impressive outcomes emerged from the study. During prophylaxis with Emicizumab all patients had experienced a reduced number of bleeding events. Moreover, a subset of these patients underwent major surgical procedures (orthopedic joint replacements) with successful outcomes. These findings underscore the potential of Emicizumab therapy as an effective option for hemophilia A patients, including those that presented a prompt production of anti-factor VIII inhibitors. As for the outcomes, this transversal study sheds some light on the positive impact of Emicizumab therapy in addressing the coagulation challenges posed by hemophilia A. The utilization of global coagulation assays, such as thrombin generation time, thrombelastography and combined with traditional coagulation assays, used as monitoring tools highlights their significance once more in evaluating the therapeutic response. This research contributes by providing physicians with valuable insights to the field, offering a potential avenue for improved patients’ care and treatment strategies that translate in enhanced quality of life for hemophilia A patients undergoing Emicizumab therapy.

Preprint REVIEW | doi:10.20944/preprints202401.1747.v1

Chronic Lymphocytic Leukemia: Prognostic/Predictive Factors in the Era of Novel Drugs

Antonio Urso, Enrica Antonia Martino, Antonio Cuneo, Massimo Gentile, Gian Matteo Rigolin

Subject: Medicine And Pharmacology, Hematology Keywords: chronic lymphocytic leukemia; BTK inhibitors; BCL-2 inhibitors; prognostic scores; predictive factors; TP53 abnormalities; IGHV; complex karyotype

Online: 24 January 2024 (13:39:51 CET)

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Preprint REVIEW | doi:10.20944/preprints202403.1155.v1

Hematological and Hemorheological Parameters of Blood Platelets as Biomarkers in Diabetes Mellitus Type 2. A Comprehensive Review

Elissaveta Zvetkova, Ivan Ivanov, Eugeni Koytchev, Nadia Antonova, Yordanka Gluhcheva, Anika Alexandrova, Georgi Kostov

Subject: Medicine And Pharmacology, Hematology Keywords: Diabetes mellitus type 2 (DM-2); blood platelets (PLTs); PLT-hematological / hemorheological / hemostatic parameters (indices); PLT "activaion" / "hyperactivation", computational modeling of PLTs, macro- and microvascular deseas (complications) in DM2

Online: 20 March 2024 (12:21:42 CET)

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Diabetes mellitus – type 2 (DM2) is a hypercoaguable state with enhanced platelets (PLTs) activation and increased clotting factor production. Simultaneously, the fibrinolytic cell system is inhibited due to formation of clots with high resistance to fibrinolysis. The stages of PLTs "activation" have been well-characterized microscopically, morphometrically and nanomechanically (by light microscope, transmisiion electron microscope - TEM, scanning electron microscope - SEM and atomic force microscope - AFM). Thrombocytes in "acivated" (pro-coagulant) state play central role in two main biological processes: hemostasis and repair of vascular vessels. Pro-coagulant PLTs participate in generation of thrombin and in development of fibrin fibers in the clot matrix. Enhanced PLTs reactivity in dibetic- (diabetes mellitus - DM2) patients is considered as a "pro-thrombotic" state. In the retrospective- and prospective- studies on the topic, the higher are the PLTs' hematometrical indices: PLTs (count), MPV (mean platelet volume), PDW (platelet distribution width), PCR (plateletcrit), as well as PLTs/Ly ratio. The PLТs parameters/indices are usefull biomarkers in early diagnosis and prognosis of DM2. The precise studies of PLTs' state of activation during DM2 might be useful for creation of new diabetes (DM2) treatment strategies and effective medicines. Using the amount of blood glucose, attached to hemoglobin (HbA1c values) as markers of glycemic control in diabetic patients, researchers have observed association between MPV and medications as Insulin, Metformin, sulphonyl ureas. Computational modellng of PLTs' activation in DM2 is also a controlling factor for thrombocyte distribution and margination in bood vessels, associated with micro- and macrovascular disease in DM2. PLTs-derived miRNAs are novel molecular biomarkers for diagnosis and prognosis of DM2, insulin resistance and diabetes complications. We have proposed (2010) oleic acid (OA) as a substance suppressing "PLT hyperactivation" and inhibiting “thrombocyte aggregation”. Antiplatelet agents, natural plant products including, could be effective in the prevention and the secondary treatment of micro- and macrovascular complications in the type 2 Diabetes mellitus. Our proposal on following up of a combination of hematological-, hemorheological- and hemostatic parameters (indices) as a new way in diagnosis, treatment, predicting and management of DM2 and its related vascular complications, probably could be more explored in future studies.

Preprint ARTICLE | doi:10.20944/preprints202401.0239.v1

Clinical Validation Of ANKRD36 Mutations As A Novel Biomarker For Monitoring Early Progression and Timely Clinical Interventions In Blast Crisis CML

Nawaf Al-anazi, Muhammad Absar, Abdulaziz Siyal, Salman Basit, Masood Shammas, Amer Mahmood, Sarah Almukhaylid, Zafar Iqbal

Subject: Medicine And Pharmacology, Hematology Keywords: Chronic Myeloid Leukemia; CML progression; Poor survival; Genomic instability; Blast crisis; ANKRD36; Clinical validation

Online: 3 January 2024 (10:25:49 CET)

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Background: Chronic Myeloid Leukemia (CML) is initiated in the bone marrow due to the chromosomal translocation t(9;22), resulting in the fusion oncogene BCR-ABL. Tyrosine kinase inhibitors (TKIs) targeting BCR-ABL have transformed fatal CML into an almost curable disease. However, TKIs lose efficacy during disease progression, and the mechanism of CML progression remains to be fully understood. Additionally, common molecular biomarkers for CML progression are lacking. Our studies previously detected ANKRD36 (c.1183_1184 delGC and c.1187_1188 dupTT) associated exclusively with advanced phase CML. However, clinical validation of this finding was pending. Therefore, this study aimed to clinically validate mutated ANKRD36 as a novel biomarker of CML progression. Materials and Methods: The study enrolled 124 patients in all phases of CML, recruited from Mayo Hospital and Hameed Latif Hospital in Lahore, Punjab, between January 2020 and September 2023. All response criteria were adopted from the European LeukemiaNet guideline 2020. Informed consent was obtained from all study subjects. The study was approved byscientific and ethical review committees of all participating centers. Sanger sequencing was employed to detect ANKRD36mutations in CML patients in accelerated phase (AP) (n=11) and blast crisis (BC) (n=10), with chronic-phase CML (CP-CML) patients as controls (n=103). Samples were processed using Big Dye Terminator Cycle Sequencing Ready Reaction kits and sequenced using ABI Prism 3730 Genetic Analyzer, and sequencing using forward and reverse primers for ANKRD36.Results: During our study, 17% of CML patients progressed to advanced phases AP-CML n= 11 (8.9%) and BC-CML n=10(8.1%). The chronic- and advanced-phase patients showed significant difference with respect to male-to-female ratio, hemoglobin level, WBC count, and platelet count. Sanger sequencing detected ANKRD36 mutations c.1183_1184 delGC and c.1187_1185 dupTT exclusively in all AP- and BC-CML patients but in none of the CP-CML patients. Nevertheless, mutations status was not associated with male-to-female ratio, hemoglobin level, WBC count, and platelet count, which makes ANKRD32 as an independent predictor of early and terminal disease progression in CML. Conclusions: The study confirms ANKRD36 as a novelgenomic biomarker for early and late CML progression. Further prospective studies should be carried out in this regard. ANKRD36, although fully uncharacterized in humans, shows the highest expression in bone marrow, particularly myeloid cells. Functional integrated genomic studies are recommended to further explore the role of ANKRD36 in the biology and pathogenesis of CML.

Preprint REVIEW | doi:10.20944/preprints202401.0466.v1

Platelets and Thrombotic Antiphospholipid Syndrome

Ibrahim Tohidi, Prabal Mittal, David Isenberg, Hannah Cohen, Maria Efthymiou

Subject: Medicine And Pharmacology, Hematology Keywords: ; Antiphospholipid syndrome; antiphospholipid antibodies; thrombosis; platelets; antiplatelets ;

Online: 5 January 2024 (07:11:24 CET)

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Preprint CASE REPORT | doi:10.20944/preprints202306.0943.v1

Hemophagocytic Lymphohistiocytosis Secondary to Hodgkin Lymphoma with Isolated Bone Marrow Involvement in a Newly Diagnosed HIV Patient

Alberto Lopez-Garcia, Laura Solan, Beatriz Alvarez, Juan Carlos Caballero, Javier Cornago, Laura Pardo, Francisco Javier Diaz de la Pinta, Raul Cordoba, Maria Rodriguez-Pinilla

Subject: Medicine And Pharmacology, Hematology Keywords: Lymphoma; HL; HIV; EBV; HLH

Online: 13 June 2023 (14:33:46 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.1996.v1

Comparison of Different Rabbit Anti-thymocyte Globulin Formulations in the Prophylaxis of Graft-versus-Host Disease: A Systematic Review

Jarosław Dybko, Ugo Giordano, Justyna Pilch, Jakub Mizera, Artur Borkowski, Monika Mordak-Domagała

Subject: Medicine And Pharmacology, Hematology Keywords: anti-thymocyte globulin; acute graft-versus-host disease; chronic graft-versus-host disease; allogeneic stem cell transplantation; thymoglobuline; grafalon

Online: 28 June 2023 (10:20:03 CEST)

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