Integrated cultivation strategies can significantly improve rice yield. However, it is unclear how integrated cultivation practices improve soil microbial diversity and its related mechanism in rice field. Therefore, four integrated cultivation practices i.e., no N application (N0), local farmers’ practice (FP), high-yield and high-efficiency practice (HYEY), and super-high-yield cultivation practice, impacts on soil properties and structure composition of microbial community were explored. Compared with N0, SHY treatment, significantly increased soil alkaline nitrogen and organic matter contents. HYHE and SHY treatments significantly altered soil fungal community and Alpha diversity. Based on Venn diagram analysis, the composition of bacterial communities under all treatments was similar, but with different compositions of fungal communities. Cluster analysis indicates that the Proteobacteria was the absolute dominant group of bacterial communities, and Chloroflexi, Bacteroidetes, and Acidobacteria were the dominant group. Different cultivation methods also led to changes in the main factors affecting soil bacteria and fungi composition. Available potassium and organic matter were the main environmental factors that affected bacteria, with the strong of available potassium on the soil. To sum up, SHY and HYEY were beneficial management options in terms of improved soil fertility Alpha diversity of the soil bacterial community respectively.

Pigs are susceptible to cold stress due to the absence of brown fat caused by the partial deletion of uncoupling protein 1 during their evolution. Some local pig breeds in China, exhibit potential cold adaptability, but research has primarily focused on fat and intestinal tissues. Skeletal muscle plays a key role in adaptive thermogenesis in mammals, yet the molecular mechanism of cold adaptation in porcine skeletal muscle remains poorly understood. This study investigated the cold adaptability of two pig breeds, Mashen pigs (MS) and Large White pigs (LW), in a 4-day cold (4°C) or room temperature (25°C) environment. We recorded phenotypic changes and collected blood, and longissimus dorsi muscle for transcriptome sequencing. Finally, a candidate gene was randomly selected for functional exploration in porcine skeletal muscle satellite cells. A decrease in body temperature and body weight in both LW and MS pigs under cold stress, accompanied by increased shivering frequency and respiratory frequency. However, MS pigs demonstrated stable physiological homeostasis, indicating a certain level of cold adaptability. LW pigs primarily responded to cold stress by regulating heat production and Glycolipid energy metabolism. MS pigs exhibited a distinct response to cold stress, involving regulation of heat production, energy metabolism pathways, robust fatty acid oxidation ability, and stronger immune response. Furthermore, the functional exploration of PRSS8 in porcine skeletal muscle satellite cells revealed it affected cellular energy metabolism and thermogenesis by regulating ERK phosphorylation. These findings shed light on the diverse transcriptional responses of skeletal muscle in LW and MS pigs under cold stress, offering valuable insights into the molecular mechanisms underlying cold adaptation in pigs.