REVIEW | doi:10.20944/preprints202007.0744.v1
Subject: Life Sciences, Biochemistry Keywords: long non-coding RNA (lncRNA); RNA polymerase II (RNAPII) transcription; gene regulation; tandem transcriptional interference (tTI); antisense transcription; DNA processing
Online: 31 July 2020 (10:47:08 CEST)
RNA polymerase II (RNAPII) frequently transcribes non-protein coding DNA sequences in eukaryotic genomes into long non-coding RNA (lncRNA). Here, we focus on the impact of the act of lncRNA transcription on nearby functional DNA units. Distinct molecular mechanisms linked to the position of lncRNA relative to the coding gene illustrate how non-coding transcription controls gene expression. We review the biological significance of the act of lncRNA transcription on DNA processing, highlighting common themes, such as mediating cellular responses to environmental changes. This review presents the background in chromatin signaling to appreciate examples in different organisms where we can interpret functions of non-coding DNA through the act of RNAPII transcription.
REVIEW | doi:10.20944/preprints202111.0203.v1
Subject: Biology, Agricultural Sciences & Agronomy Keywords: flower development; epigenetics; RNA biology; Genomics; single cell biology
Online: 10 November 2021 (11:00:03 CET)
The rise of data science in biology stimulates interdisciplinary collaborations to address fundamental questions. Here, we report the outcome of the first SINFONIA symposium focused on revealing the mechanisms governing plant reproductive development across biological scales. The intricate and dynamic target networks of known regulators of flower development remain poorly understood. To analyze development from the genome to the final floral organ morphology, high-resolution data that capture spatiotemporal regulatory activities are necessary and require advanced computational methods for analysis and modeling. Moreover, frameworks to share data, practices and approaches that facilitate the combination of varied expertise to advance the field are called for. Training young researchers in interdisciplinary approaches and science communication offers the opportunity to establish a collaborative mindset to shape future research.