Cellulase and Xylanase from Phanerochaete chrysosporium ATCC24725: Production, Regulation of Synthesis, and Some Properties

In this study, wood-rotting basidiomycetes were screened for cellulase and xylanase production in submerged fermentation of crystalline cellulose and lignocellulosic materials. Phanerochaete chrysosporium ATCC 24725 was selected as the most promising enzyme producer, and the physiological mechanisms controlling the synthesis of cellulase and xylanase were elucidated. The inducible nature of cellulase synthesis and the influence of catabolite repression were characterized. The enzyme preparation isolated after culturing the fungus in a fermenter was partially purified and characterized. The obtained results highlight the potential of P. chrysosporium ATCC 24725 as a reliable fungal cellulase producer for biorefinery applications.