Submitted:
06 August 2026
Posted:
07 August 2026
You are already at the latest version
Abstract
Keywords:
1. Introduction
2. Materials and Methods
2.1. Experimental Site
2.2. Experimental Shading-Treatment Design
2.3. Microclimate and Meteorological Monitoring
2.4. Analysis of Chlorophyll a Fluorescence
2.5. Observations of Foliage and Floral Bud Damage and Estimation of Flowering Intensity
2.6. Whole-Tree Carbon-Uptake Measurements
2.7. Tree Yield and Average Fruit Weight
2.8. Statistical Analysis
3. Results
3.1. Year 1
3.1.1. Effect of Shading Nets on Air Temperature
3.1.2. Effect of Shading Nets on Flowering Intensity, Fruit Yield, and Fruit Weight
3.2. Year 2
3.2.1. Effect of Shading Nets on Air Temperature and Relative Humidity
3.2.2. Frost Protection by the Shading Nets
3.2.3. Effect of Shading Nets on Whole-Tree Carbon Uptake and Maximum Quantum Yield (Fv/Fm)
3.2.4. Effect of Shading Nets on Flowering Intensity, Fruit Yield, and Fruit Weight
3.3. Across-Year Analysis of Flowering Intensity, Fruit Yield, and Fruit Weight
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
| a.s.l. | Above Sea Level |
| NPK | Nitrogen–Phosphorus–Potassium |
| PAR | Photosynthetically Active Radiation |
| PPFD | Photosynthetic Photon Flux Density |
| PSII | Photosystem II |
| SE | Standard Error |
| USD | United States Dollar |
References
- Alcaraz, M.L.; Thorp, T.G.; Hormaza, J.I. Phenological Growth Stages of Avocado (Persea Americana) According to the BBCH Scale. Sci. Hortic. 2013, 164, 434–439. [CrossRef]
- Silva, T.A.; Ledesma, N. Avocado History, Biodiversity and Production. In Sustainable Horticultural Systems; Nandawani, D., Ed.; Springer International Publishing, Switzerland, 2014; Vol. 2, pp. 157–205 ISBN 978-3-319-06903-6.
- Ballen, F.H.; Evans, E.; Parra-Acosta, Y.K. Consumer Preferences for Green Skin Avocados in the US Market: The Role of Experienced Quality Attributes, Credence Attributes, and Demographic Factors. J. Agric. Food Ind. Organ. 2021, 20, 1–9. [CrossRef]
- Charrier, G.; Ngao, J.; Saudreau, M.; Améglio, T. Effects of Environmental Factors and Management Practices on Microclimate, Winter Physiology, and Frost Resistance in Trees. Front. Plant Sci. 2015, 6, 1–18. [CrossRef]
- Perry, K.B. Basics of Frost and Freeze Protection for Horticultural Crops. Horttechnology 1998, 8, 10–15.
- Jouyban, Z.; Hasanzade, R.; Sharafi, S. Chilling Stress in Plants. International Journal of Agriculture and Crop Sciences 2013, 5, 2961–2968.
- Jones, H.G. Frost Protection: Fundamentals, Practice, and Economics. Volume 1. Exp. Agric. 2006, 42, 369. [CrossRef]
- Verslues, P.E.; Agarwal, M.; Katiyar-Agarwal, S.; Zhu, J.; Zhu, J.K. Methods and Concepts in Quantifying Resistance to Drought, Salt and Freezing, Abiotic Stresses That Affect Plant Water Status. Plant Journal 2006, 45, 523–539. [CrossRef]
- De Melo-Abreu, J.P.; Villalobos, F.J.; Mateos, L. Frost Protection. In Principles of Agronomy for Sustainable Agriculture; Villalobos, F.J., Fereres, E., Eds.; Springer International Publishing: Cham, 2016; pp. 443–457 ISBN 978-3-319-46116-8.
- Janda, T.; Szalai, G.; Rios-Gonzalez, K.; Veisz, O.; Páldi, E. Comparative Study of Frost Tolerance and Antioxidant Activity in Cereals. Plant Science 2003, 164, 301–306. [CrossRef]
- Bar-Noy, Y.; Sofer-Arad, C.; Perel, M.; Cohen, H.; Senesh, N.; Noy, M.; Rubinovich, L. Frost Protection Efficiency Evaluation in Avocado with a Horizontal Wind Machine. Fruits 2019, 74, 124–129. [CrossRef]
- Lahack, M.; Noy, M.; Szenes, N.; Rubinovich, L. Vegetative Growth, Flowering, and Physiological Parameters of Young Avocado “Hass” and ’Hass’-Like Cultivars Under Extreme Weather Conditions. International Journal of Fruit Science 2025, 25, 378–392. [CrossRef]
- Weil, A.; Sofer-Arad, C.; Bar-Noy, Y.; Liran, O.; Rubinovich, L. Comparative Study of Leaf Antioxidant Activity as a Possible Mechanism for Frost Tolerance in ‘Hass’ and ‘Ettinger’ Avocado Cultivars. J. Agric. Sci. 2019, 157, 342–349. [CrossRef]
- Joshi, N.C.; Yadav, D.; Ratner, K.; Kamara, I.; Aviv-Sharon, E.; Irihimovitch, V.; Charuvi, D. Sodium Hydrosulfide Priming Improves the Response of Photosynthesis to Overnight Frost and Day High Light in Avocado (Persea Americana Mill, Cv. ‘Hass’). Physiol. Plant. 2019, 168, 394–405. [CrossRef]
- Zhang, X.; Lu, C.; Guan, Z. Weakened Cyclones, Intensified Anticyclones and Recent Extreme Cold Winter Weather Events in Eurasia. Environmental Research Letters 2012, 7, 044044. [CrossRef]
- Ghaemi, A.A.; Rafiee, M.R.; Sepaskhah, A.R. Tree-Temperature Monitoring for Frost Protection of Orchards in Semi-Arid Regions Using Sprinkler Irrigation. Agric. Sci. China 2009, 8, 98–107. [CrossRef]
- Ribeiro, A.C.; De Melo-Abreu, J.P.; Snyder, R.L. Apple Orchard Frost Protection with Wind Machine Operation. Agric. For. Meteorol. 2006, 141, 71–81. [CrossRef]
- Manja, K.; Aoun, M. The Use of Nets for Tree Fruit Crops and Their Impact on the Production: A Review. Sci. Hortic. 2019, 246, 110–122. [CrossRef]
- Shahak, Y. Photoselective Netting: An Overview of the Concept, R&D and Practical Implementation in Agriculture. Acta Hortic. 2014, 1015, 155–162. [CrossRef]
- Shahak, Y.; Ratner, K.; Giller, Y.E.; Zur, N.; Or, E.; Gussakovsky, E.E.; Stern, R.; Sarig, P.; Raban, E.; Harcavi, E.; et al. Improving Solar Energy Utilization, Productivity and Fruit Quality in Orchards and Vineyards by Photoselective Netting. In Proceedings of the Acta Horticulturae; International Society for Horticultural Science, 2008; Vol. 772, pp. 65–72.
- Zait, Y.; Elingold, I.; Londener, A.; Gal, E.; Or, G.; Galpaz, N. Banana Frost Protection by Thermal Nets. In Proceedings of the Acta Horticulturae; International Society for Horticultural Science: Istanbul, Turkey, March 23 2020; Vol. 1272, pp. 21–26.
- Chernoivanov, S.; Neuberger, I.; Levy, S.; Szenes, N.; Rubinovich, L. Covering Young ‘Reed’ Avocado Trees with Shading Nets during Winter Alleviates Cold Stress and Promotes Vegetative Growth. Eur. J. Hortic. Sci. 2022, 87, 1–10. [CrossRef]
- Rubinovich, L.; Sofer-Arad, C.; Chernoivanov, S.; Szenes, N. Effects of Covering Mature Avocado ‘Pinkerton’ Trees with High-Density Shading Nets during Cold Winters on Microclimate, Chlorophyll Fluorescence, Flowering, and Yield. HortScience 2023, 58, 1201–1204. [CrossRef]
- Lahak, M.; Alon, E.; Chen, A.; Rubinovich, L. Covering Young Avocado ‘Hass’ Trees with High-Density Shading Nets during the Winter Mitigates Frost Damage and Improves Tree Performance. Trees 2024, 38, 327–338. [CrossRef]
- Mditshwa, A.; Magwaza, L.S.; Tesfay, S.Z. Shade Netting on Subtropical Fruit: Effect on Environmental Conditions, Tree Physiology and Fruit Quality. Sci. Hortic. 2019, 256, 108556. [CrossRef]
- Alon, E.; Shapira, O.; Azoulay-Shemer, T.; Rubinovich, L. Shading Nets Reduce Canopy Temperature and Improve Photosynthetic Performance in ‘Pinkerton’ Avocado Trees during Extreme Heat Events. Agronomy 2022, 12, 1360. [CrossRef]
- Schreiber, U.; Schliwa, U.; Bilger, W. Continuous Recording of Photochemical and Non-Photochemical Chlorophyll Fluorescence Quenching with a New Type of Modulation Fluorometer. Photosynth. Res. 1986, 10, 51–62. [CrossRef]
- Kramer, D.M.; Johnson, G.; Kiirats, O.; Edwards, G.E. New Fluorescence Parameters for the Determination of QA Redox State and Excitation Energy Fluxes. Photosynth. Res. 2004, 79, 209–218. [CrossRef]
- Baker, N.R. Chlorophyll Fluorescence: A Probe of Photosynthesis in Vivo. Annu. Rev. Plant Biol. 2008, 59, 89–113. [CrossRef]
- Ziv, D.; Zviran, T.; Zezak, O.; Samach, A.; Irihimovitch, V. Expression Profiling of FLOWERING LOCUS T-like Gene in Alternate Bearing ‘Hass’ Avocado Trees Suggests a Role for PaFT in Avocado Flower Induction. PLoS One 2014, 9, 1–14. [CrossRef]
- Rog, I.; Hilman, B.; Fox, H.; Yalin, D.; Qubaja, R.; Klein, T. Increased Belowground Tree Carbon Allocation in a Mature Mixed Forest in a Dry versus a Wet Year. Glob. Chang. Biol. 2024, 30, e17172. [CrossRef]
- Klein, T.; Hoch, G. Tree Carbon Allocation Dynamics Determined Using a Carbon Mass Balance Approach. New Phytologist 2015, 205, 147–159. [CrossRef]
- Klein, T.; Rotenberg, E.; Tatarinov, F.; Yakir, D. Association between Sap Flow-Derived and Eddy Covariance-Derived Measurements of Forest Canopy CO2 Uptake. New Phytologist 2016, 209, 436–446. [CrossRef]
- Gutiérrez-Gamboa, G.; Araya-Alman, M.; Romero-Bravo, S.; Carrasco-Benavides, M.; Verdugo-Vásquez, N.; Chacón-Fuentes, M. Shading Nets Modified Cluster-Zone Radiation, Bunch Sunburn Percentage and Berry Amino Acid Content in Cabernet Sauvignon: A Preliminary Study. Plants 2026, 15, 2183. [CrossRef]
- Wise, R.R. The Production, Action and Study of Reactive Oxygen Species during Chilling in the Light. Photosynth. Res. 1995, 45, 79–97.
- Chung, S.W.; Rho, H.; Lim, C.K.; Jeon, M.K.; Kim, S.; Jang, Y.J.; An, H.J. Photosynthetic Response and Antioxidative Activity of ‘Hass’ Avocado Cultivar Treated with Short-Term Low Temperature. Sci. Rep. 2022, 12, 11593. [CrossRef]
- Yoshida, S.; Sakai, A. The Effect of Thawing Rate on Freezing Injury in Plants. II. The Change in the Amount of Ice in Leaves as Produced by the Change in Temperature. Low Temperature Science, Series Biological Sciences 1968, B 26, 23–31.
- Snyder, R.L. (Richard L.); Melo-Abreu, J.P. de. Frost Protection : Fundamentals, Practice and Economics; Food and Agriculture Organization of the United Nations, 2005; ISBN 9251053286.
- Gusta, L. V; Fowler, D.B. Factors Affecting the Cold Survival of Winter Cereals. Can. J. Plant Sci 1976, 57, 219–231.
- Mickelbart, M. V.; Robinson, P.W.; Witney, G.; Arpaia, M.L. “Hass” Avocado Tree Growth on Four Rootstocks in California. II. Shoot and Root Growth. Sci. Hortic. 2012, 143, 205–210. [CrossRef]
- Lovatt, C.J. Alternate Bearing Of “Hass” Avocado. California Avocado Society Yearbook 2010, 93, 125–140.
- Pochamreddy, M.; Haim, D.; Halon, E.; Keinan, E.; Rai, A.C.; Kamara, I.; Sadka, A.; Irihimovitch, V. Alternate Bearing in ‘Hass’ Avocado: Fruit Load-Induced Changes in Bud Auxin Homeostasis Are Associated with Flowering Repression. J. Exp. Bot. 2024, 75, 5717–5733. [CrossRef]
- Lahack, M.; Szenes, N.; Rubinovich, L. Effects of Uniconazole Treatment on ‘Hass’ Avocado Productivity and Gas-Exchange Parameters under Mediterranean Climate. Front. Plant Sci. 2025, 16, 1–13. [CrossRef]
- Mupambi, G.; Musacchi, S.; Serra, S.; Kalcsits, L.A.; Layne, D.R.; Schmidt, T. Protective Netting Improves Leaf-Level Photosynthetic Light Use Efficiency in ‘Honeycrisp’ Apple under Heat Stress. HortScience 2018, 53, 1416–1422. [CrossRef]
- El-Naby, S.K.M.A.; Esmail, A.M.A.M.; Baiea, M.H.M.; Amin, O.A.E.F.; Mohamed, A.A.A. Mitigation of Heat Stress Effects by Using Shade Net on Washington Navel Orange Trees Grown in Al-Nubaria Region, Egypt. Acta Scientiarum Polonorum, Hortorum Cultus 2020, 19, 15–24. [CrossRef]
- Mira-García, A.B.; Conejero, W.; Vera, J.; Ruiz-Sánchez, M.C. Leaf Water Relations in Lime Trees Grown under Shade Netting and Open-Air. Plants 2020, 9, 510. [CrossRef]
- Boini, A.; Bortolotti, G.; Perulli, G.D.; Venturi, M.; Bonora, A.; Manfrini, L.; Corelli Grappadelli, L. Gala Apple Production Benefits from High Shading Levels and Water Limitation, under Exclusion Netting. Sci. Hortic. 2023, 310, 111756. [CrossRef]
- Stern, R.A.; Rozen, A.; Eshed, R.; Zviran, T.; Sisai, I.; Sherman, A.; Irihimovitch, V.; Sapir, G. Bumblebees (Bombus Terrestris) Improve ‘Hass’ Avocado (Persea Americana) Pollination. Plants 2021, 10, 1372. [CrossRef]







| Month | Dec-23 | Jan-24 | Feb-24 | Mar-24 | Apr-24 | May-24 | Jun-24 | Jul-24 |
| Irrigation (m3 ha-1) | 0 | 0 | 30 | 128 | 292 | 186 | 942 | 1306 |
| Month | Aug-24 | Sep-24 | Oct-24 | Nov-24 | Dec-24 | Jan-25 | Feb-25 | Mar-25 |
| Irrigation (m3 ha-1) | 1276 | 1620 | 952 | 480 | 0 | 382 | 90 | 254 |
| Month | Apr-25 | May-25 | Jun-25 | Jul-25 | Aug-25 | Sep-25 | Oct-25 | Nov-25 |
| Irrigation (m3 ha-1) | 740 | 940 | 1900 | 1820 | 1838 | 1592 | 912 | 638 |
| Month | Dec-25 | Jan-26 | ||||||
| Irrigation (m3 ha-1) | 60 | 26 |
| Flowering intensity | Fruit yield | Fruit weight | |
| Treatment | * | ns | ns |
| Year | ** | ** | * |
| Treatment x Year | ns | ns | ns |
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