Female rugby league performance is influenced by multiple interacting systems; however, the extent to which sensory and autonomic function differentiates playing level remains unclear. This study investigated whether visual, vestibular, somatosensory, and auto-nomic performance differ by playing level and positional group in female rugby league athletes. Elite and sub-elite athletes completed lower-limb proprioception testing using an Active Movement Extent Discrimination Assessment protocol, alongside visual–vestibular and autonomic measures obtained via a virtual-reality eye-tracking system. Bayesian hierarchical models were used to examine the effects of playing level, positional group (adjustables, backs, forwards), and their interaction, with posterior inference based on probability of direction and region of practical equivalence analyses. Interaction effects between level and position were observed for selected variables across somatosensory, vestibulo-oculomotor, and autonomic domains. Elite adjustables demonstrated higher ankle proprioceptive acuity than sub-elite adjustables (PD = 0.94), with additional interaction effects identified for vestibulo-oculomotor time on target (PD = 0.95) and autonomic dilation velocity (PD = 0.98). However, findings were not consistent across positional groups or outcome measures, and substantial within-group variability was evident. Overall, sensory and autonomic performance did not consistently differentiate playing level, suggesting limited utility for cross-sectional discrimination but potential value for longitudinal, individualised athlete monitoring.