Accurate information on atmospheric temperature profiles is crucial for improving numerical weather forecasting and short-term numerical weather prediction (NWP). However, the harsh environment of the Tibetan Plateau (TP) limits the availability of station observations, which fails to meet the high spatial resolution required for NWP. In this study, we present a method to calibrate temperature profiles obtained from the Vertical Atmosphere Sounding System (VASS) using data from the polar-orbiting satellite FY-3C. The aim is to provide high-resolution atmospheric structure for NWP in the TP. The temperature profile in VASS exhibits temporal and spatial heterogeneity due to the significant impact of clouds on the radiative transfer mode (RTM). To address this, we employ a combination of variation and artificial neural network (Var-ANN) methods to calibrate the satellite product and improve its compatibility with the model. To confirm the feasibility of our method, we compare the calibrated results with the observed data from 121 radiosonde soundings and 2400 meteorological stations in China, both of which represent conditions closest to the real atmospheric states. The calibrated temperature shows improvements over the original temperature, with a root mean square error, bias, and agreement with radiosonde soundings of 2.11, -0.72, and 0.998, respectively. We also select two classical cases involving the eastward movement of the plateau vortex (PV) and the formation of precipitation to verify the applicability of the calibration in NWP. The results demonstrate that the performance of NWP improves after assimilating the calibrated data, with the Var-ANN data assimilation scheme achieving the highest threat score of 66.9 and 66.7 for case 1 and case 2, respectively. These findings suggest that the Var-ANN method is suitable for calibrating satellite temperature profiles, and the calibrated data holds potential for precipitation forecasting. Furthermore, the novel method can also be applied in global temperature profile correction and satellite cross-calibration.