Fully Complementary Interactions of LmiRNA with mRNA of Human Genes

Londin et al. discovered miRNAs (LmiRNAs) whose properties had been little studied for unknown reasons. In this study, we examined fully complementary interactions of LmiRNAs with mRNAs of human genes. Using the MirTarget program, a significant number of target genes with unique properties of interaction with LmiRNAs were identified. Of 3707 LmiRNAs in the mRNAs of 75 target genes, the sites of fully complementary LmiRNA binding were located in the 5'UTR with a high free energy of interaction. In the mRNAs of 81 target genes, the fully complementary LmiRNA binding sites (BSs) were located in the CDS. Only seven LmiRNAs bound in the 3'UTRs of target genes. The KIFC3, PHF15, RPL15 and SNX11 genes were identified, encoding LmiRNA-5p and LmiRNA-3p, which bind to the mRNAs of these genes. The mRNA of most genes were bound by only one LmiRNA. The BMP8B, BMP8B, FGFRL1 and SDC3 genes were identified, whose mRNAs bound the pair ID00121.5p and ID02992.5p. These results expand our knowledge of LmiRNAs and allow us to recommend these LmiRNAs as diagnostic and therapeutic agents for various diseases.