Submitted:
14 January 2026
Posted:
15 January 2026
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Abstract
An archaeological exposure near Hachum, featuring a Ditch profile interpreted as part of a Neolithic earthwork, was characterized using DNA analyses of bacterial 16S rRNA from soil samples. The results showed that the middle and lower parts of the Ditch fill could be clearly distinguished from each other and from the surrounding area based on the composition of soil bacterial DNA. Genera detected predominantly in the lower part of the Ditch suggest that, after the Ditch was completed, organic matter, animal dung, and possibly even human feces were accumulated at the bottom. The investigations demonstrate that analyses of soil bacterial communities can provide valuable insights into the history and function of a Neolithic earthwork and, more generally, represent an important additional source of information for interpreting archaeological contexts that are devoid of or poor in finds.