Submitted:
08 September 2025
Posted:
09 September 2025
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Abstract
Keywords:
1. Introduction
2. Methods
2.1. Study Setting and Participants
2.2. Sampling and Sample Size
2.3. Case and Control Definitions and Eligibility Criteria
- Inclusion Criteria
- Exclusion criteria:
2.4. Recruitment Procedure for Eligible Subjects

2.5. Exposures and Covariates
- Main exposure: Maternal dietary diversity was measured using the Diet Quality Questionnaire (DQQ), dietary diversity score (DDS; number of distinct food groups consumed), and the minimum binary dietary diversity for women (MDD-W, DDS ≥ 5).
-
Other exposures/covariates:
- -
- Sociodemographic and economic data: maternal age (years), maternal education, marital status, maternal education level, maternal occupation, husband’s smoking status at home, and household wealth index, food security, unintended pregnancy, and facility identifier;
- -
- Maternal obstetric and health characteristics: parity, history of LBW, number of prenatal care visits (PCCs), maternal smoking status, gestational age at first PC, iron folate supplementation, hemoglobin level (mg/dL), blood pressure (mm Hg), HIV status, malaria during pregnancy;
- -
- Maternal nutritional characteristics and behavior: prior nutrition education, maternal height (cm), mid-upper arm circumference (MUAC, in mm), maternal weight in the first, second, and third trimesters of pregnancy (in kg), intermittent preventive treatment (IPT), number of IPT doses, and maternal alcohol consumption.
2.6. Data Collection
- The mothers were interviewed to collect sociodemographic data, maternal dietary diversity, lifestyle, prenatal follow-up, maternal history, and pregnancy intention.
- Medical records (partogram and prenatal consultation form) were used to triangulate specific data collected during the interview and to collect data on the child’s birth weight, gestational age, prenatal visits, IPT for malaria, last-trimester hemoglobin (Hb) level (measured using a hemoglobinometer), and malaria, which was assessed using a thick drop test in the laboratory. Self-reports were cross-referenced with prenatal records.
- Direct measurements: Maternal anthropometry (height (cm), measured using a height chart; mid-upper arm circumference, measured using the MUAC, with a threshold of 230 mm).
-
Dietary diversity measurement: Maternal dietary diversity was measured using two complementary methods.
- -
- Maternal dietary diversity was determined from the Diet Quality Questionnaire (DQQ), which was adapted to the DRC context, and enabled a qualitative recall of consumption over the last 24 h (MDD-W, FAO/WHO). The 10 food groups were (1) basic starchy foods; (2) legumes; (3) nuts/seeds; (4) dairy products; (5) meat products; (6) eggs; (7) dark green leafy vegetables; (8) other fruits/vegetables rich in vitamin A; (9) other vegetables; and (10) other fruits. A composite women’s dietary diversity score (SDAF) was calculated based on the number of food groups consumed by each mother, with a scale ranging from 0 (no consumption) to 10 (consumption of all groups). A score of ≥5 groups is considered to indicate adequate dietary diversity, while a score of <5 groups reflects inadequate dietary diversity [26].
- -
- Weekly recall (modified MDD-W): Using a qualitative recall of food-group consumption (MDD-W, modified FAO), the frequency of habitual consumption of food groups ≥ 3 times per week was assessed. A composite women’s dietary diversity score (SDAF) was adapted according to the country context, with a scale ranging from 0 (no consumption) to 9 (consumption of all groups). A score of ≥5 is considered to indicate adequate dietary diversity, while a score of <5 reflects inadequate dietary diversity [12].
2.7. Statistical Analysis
2.8. Ethical Considerations
3. Results
3.1. Participant Characteristics
3.2. Dietary Diversity
3.3. Food-Group Consumption
3.4. Determinants of Low Birth Weight
4. Discussion
4.1. Key Findings and Implications
4.2. Addressing Malaria and Other Determinants
4.3. Recommendations for Policy and Practice
4.4. Limitations and Future Research
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of interest
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| Characteristics | Case (n = 64) n (%) |
Control (n = 128) n (%) |
Total (n = 192) n (%) |
p-Value |
|---|---|---|---|---|
| Distribution of mothers by sociodemographic characteristics | ||||
| Mother’s age (years) (mean ± SD) | 25.98 ± 5.21 | 28.35 ± 5.65 | 27.56± 5.61 | 0.0056 |
| Age group (years) | 0.127 | |||
| Less than 25 | 22 (34.38) | 33 (25.78) | 55 (28.65) | |
| 25–34 | 39 (60.94) | 78 (60.94) | 117 (60.94) | |
| 35 and above | 3 (4.69) | 17 (13.28) | 20 (10.42) | |
| Education level | 0.007 | |||
| Below secondary school | 27 (42.19) | 30 (23.44) | 57 (29.69) | |
| Secondary school and above | 37 (57.81) | 98 (76.56) | 135 (70.31) | |
| Marital status | 0.001 | |||
| Single | 17 (26.56) | 10 (7.81) | 27 (14.06) | |
| Married/in union | 46 (71.88) | 118 (92.19) | 164 (85.42) | |
| Others | 1 (1.56) | 0 (0.00) | 1 (0.52) | |
| Occupation | 0.032 | |||
| Housewife | 40 (62.50) | 76 (59.38) | 116 (60.42) | |
| Unemployed | 11 (17.19) | 9 (7.03) | 20 (10.42) | |
| Employee | 13 (20.31) | 43 (33.59) | 56 (29.17) | |
| Unwanted pregnancy | <0.001 | |||
| No | 32 (50.00) | 22 (17.19) | 54 (28.12) | |
| Yes | 32 (50.00) | 106 (82.81) | 138 (71.88) | |
| Passive smoking | 0.043 | |||
| Yes | 14 (21.88) | 14 (10.94) | 28 (14.58) | |
| No | 50 (78.12) | 114 (89.06) | 164 (85.42) | |
| Socioeconomic status | 0.092 | |||
| Poor | 28 (43.75) | 37(28.91) | 65 (33.85) | |
| Middle | 26 (40.62) | 59 (46.09) | 85 (44.27) | |
| Rich | 10 (15.62) | 32 (25.00) | 42 (21.88) | |
| Food security level | 0.123 | |||
| Food insecurity | 41(64.06) | 67 (52.34) | 108 (56.25) | |
| Food security | 23 (35.94) | 61 (47.66) | 84 (43.75) | |
| Mother’s obstetrics and health-related characteristics | ||||
| Number of ANC | 0.006 | |||
| <4 | 33 (51.56) | 40 (31.25) | 73 (38.02) | |
| ≥4 | 31 (48.44) | 88 (68.75) | 119 (61.98) | |
| Pregnancy age at 1st ANC (week) | 0.186 | |||
| Over 16 | 49 (76.56) | 108 (84.38) | 157 (81.77) | |
| Before 16 | 15 (23.44) | 20 (15.62) | 35 (18.23) | |
| History of malaria during pregnancy | 0.024 | |||
| Yes | 43 (67.19) | 64 (50.00) | 107 (55.73) | |
| No | 21 (32.81) | 64 (50.00) | 85 (44.27) | |
| Hemoglobin level (mg/dL) | 0.019 | |||
| <12 | 38 (59.38) | 53 (41.41) | 91 (47.40) | |
| ≥12 | 26 (40.62) | 75 (58.59) | 101 (52.60) | |
| Number of previous births (parity) | <0.001 | |||
| Primiparous | 35 (54.69) | 33 (25.78) | 68 (35.42) | |
| 2–3 | 24 (37.50) | 71 (55.47) | 95 (49.48) | |
| Over 3 | 5 (7.81) | 24 (18.75) | 29 (15.10) | |
| History of low birth weight | 0.504 | |||
| Yes | 8 (12.50) | 12 (9.38) | 20 (10.42) | |
| No | 56 (87.50) | 116 (90.62) | 172 (89.58) | |
| History of hypertension | 0.586 | |||
| Yes | 3 (4.69) | 4 (3.12) | 7 (3.65) | |
| No | 61 (95.31) | 124 (96.88) | 185 (96.35) | |
| Mother’s nutritional and behavioral factor characteristics | ||||
| Mother’s height (cm) | 0.200 | |||
| <150 | 3 (4.69) | 2 (1.56) | 5 (2.60) | |
| ≥150 | 61 (95.31) | 126 (98.44) | 187 (97.40) | |
| Maternal MUAC (mm) | <0.001 | |||
| <230 | 14 (21.88) | 5 (3.91) | 19 (9.90) | |
| ≥230 | 50 (78.12) | 123 (96.09) | 173 (90.10) | |
| Nutritional education | ||||
| No | 8 (12.50) | 1 (0.78) | 9 (4.69) | <0.001 |
| Yes | 56 (87.50) | 127 (99.22) | 183 (95.31) | |
| Have you ever taken IPT | 0.294 | |||
| Yes | 56 (87.50) | 118 (92.19) | 174 (90.62) | |
| No | 8 (12.50) | 10 (7.81) | 18 (9.38) | |
| How many times have you taken | 0.123 | |||
| No | 8 (12.50) | 10 (7.81) | 18 (9.38) | |
| Less than 3 | 51 (79.69) | 95 (74.22) | 146 (76.04) | |
| 3 and above | 5 (7.81) | 23 (17.97) | 28 (14.58) | |
| Have you ever drunk alcohol | 0.225 | |||
| Yes | 18 (28.12) | 26 (20.31) | 44 (22.92) | |
| No | 46 (71.88) | 102 (79.69) | 148 (77.08) | |
| Minimum Dietary Diversity for Women (MDD-W 24 h recall) | ||||
| Inadequate | 12 (18.75) | 21 (26.41) | 33 (17.19) | 0.685 |
| Adequate | 52 (81.25) | 107 (83.59) | 159 (82.81) | |
| Modified MDD-W (weekly) | <0.001 | |||
| Inadequate | 37 (57.8) | 36 (28.1) | ||
| Adequate | 27 (42.2) | 92 (71.9) |
| Dietary Diversity Indicator | Case (Low Birth Weight, n = 64) | Control (Weight > 2500 g, n = 128) | p-Value |
|---|---|---|---|
| DDS (24 h) mean ± SD | 5.36 ± 1.19 | 5.77 ± 1.37 | 0.0407 |
| Reached MDD-W (24 h) (%) | 81.2% (n = 52) | 83.6% (n = 107) | 0.685 |
| DDS (weekly) mean ± SD | 4.48 ± 1.63 | 5.21 ± 1.31 | <0.001 |
| Reached MDD-W (weekly) | 42.2% (n = 27) | 71.9% (n = 92) | <0.001 |
| Discrepancies (24 h vs. weekly) | 51.6% (n = 33) | 32.0% (n = 41) | 0.009 |
| Variable | Non Adjusted OR [95% CI] |
p-Value | Adjusted OR [95% CI] |
p-Value |
|---|---|---|---|---|
| MDD-W (DDS ≥ 5) | 0.85 [0.39–1.86] | 0.685 | 0.82 [0.32–2.07] | 0.678 |
| Parity | 0.67 [0.52–0.87] | 0.002 | 0.70 [0.42–1.18] | 0.182 |
| Maternal Height (cm) | 0.87 [0.82–0.92] | <0.001 | 0.85 [0.79– 0.92] | <0.001 |
| MUAC < 230 mm | 6.89 [2.36–20.14] | <0.001 | 5.29 [1.40–19.91] | 0.014 |
| Malaria during pregnancy | 2.05 [1.09–3.83] | 0.025 | 2.04 [0.92–4.52] | 0.079 |
| Unwanted pregnancy | 0.21 [0.11–0.41] | <0.001 | 0.17 [0.071–0.385] | <0.001 |
| Nutritional education | 0.055 [0.007–0.45] | 0.007 | 0.032 [0.003–0.364] | 0.006 |
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