Submitted:
23 January 2025
Posted:
24 January 2025
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Abstract
Objective To investigate the pubertal development in obese school-age children and explore the associated factors. Method A total of 217 school-aged children, aged 6-12 years, visiting the clinic at Beijing Jishuitan Hospital, Capital Medical University, were enrolled in a study to complete a series of examinations. These assessments included body composition analysis, blood glucose levels, fasting insulin levels, and evaluations of secondary sexual characteristic development. Statistical analysis was conducted using R4.0.3 software. Result There were 152 male students, with 83 (54.6%) in the adolescent undeveloped group and 69 (45.4%) in the adolescent developed group. There were 119 female students, with 30 (25.2%) in the adolescent undeveloped group and 89 (74.8%) in the adolescent developed group. In a comparative analysis of children, those in the adolescent development group exhibited significantly higher values for age, height, weight, body mass index (BMI), BMI-z score, body fat, muscle mass, fat-free weight, fat-free body mass index, and waist-hip ratio compared to the adolescent undeveloped group (P < 0.05). Additionally, fasting insulin and insulin resistance index were also higher in the development group, with statistical significance. BMI emerged as an independent factor affecting the adolescent development of obese school-age girls (P < 0.05). Among boys in the adolescent development group, the consumption of sugary drinks and fried food was higher than in the undeveloped group, and moderate-intensity exercise was significantly lower, with statistical significance (P < 0.05). In girls, the adolescent development group reported longer daily sitting times and higher intakes of sugary drinks and fried foods compared to the undeveloped group, with these differences being statistically significant (P < 0.05). The consumption of fried food was positively correlated with adolescent development in obese children and was identified as an independent influencing factor of adolescent development (P < 0.05). Conclusion 6-12-year-old Obese children who have entered puberty exhibit increased body fat, fat-free body mass index, and waist-hip ratio. There is a positive correlation between BMI and the onset of puberty in obese girls, and a high intake of fried food may accelerate the pubertal development in these children.
Keywords:
: obesity
; puberty
; body composition
; children
Obesity among children and adolescents has emerged as a paramount issue in global health. Over the past 40 years, there has been a marked increase in the prevalence of obesity among children of all ages globally [0]. The disparity between high caloric intake and low energy expenditure results in a surplus of energy, which leads to the accumulation of adipose tissue. The etiology of obesity is multifaceted, encompassing nutritional factors, developmental stages, behavioral patterns, genetic predispositions, environmental influences, and even epigenetic factors and in utero effects. Childhood obesity not only elevates the long-term risk of cardiovascular diseases, dyslipidemia, obstructive sleep apnea, certain cancers, and premature mortality but also poses detrimental effects on the reproductive system. These effects can manifest as precocious puberty, irregular menstrual cycles, polycystic ovary syndrome, and an increased propensity for high-risk sexual behaviors [2]. An increasing body of research indicates that children with obesity tend to initiate puberty at an earlier age compared to their normal-weight peers [3]. Obesity and early-onset puberty are significant risk factors for the development of metabolic syndrome, type 2 diabetes, and insulin resistance [4]. Puberty development is influenced by a multitude of factors, including heredity, nutrition, environment, and lifestyle behaviors. Among these, metabolic conditions and energy reserves are pivotal in regulating the onset of puberty. There is robust evidence supporting the impact of higher body mass index (BMI) on both the initiation and progression of puberty in both boys and girls. The age at which girls experience breast development is progressively earlier and is positively correlated with BMI. Additionally, obese boys of the same age are more likely to undergo sexual maturation than their non-obese counterparts [5,6,7]. However, some studies have indicated a negative linear correlation between BMI and the onset of puberty in males, suggesting that individuals with early puberty development tend to have a lower percentage of body fat [8,9]. The factors influencing the pubertal development of obese children and the underlying mechanisms remain inconclusive. To address this gap, we conducted a study observing the sexual maturation and lifestyle behaviors of 271 obese children aged 6-12. We analyzed potential influencing factors on the pubertal development of these children and investigated the possible mechanisms by which body fat might participate in the activation of the gonadal axis. The findings of this research are presented below.
1. Materials and Methods
1.1. Research Objects and Groups
The study subjects consisted of obese children aged 6-12 who underwent physical examinations at the Pediatrics Department of Beijing Jishuitan Hospital, Capital Medical University, between October 2018 and December 2023. The diagnostic criteria for childhood obesity were based on the body mass index (BMI) cut-off points recommended by the Expert Group on 'Evaluation, Treatment, and Prevention of Childhood Obesity in China' [10]. Exclusion criteria included: ① Children with liver or kidney dysfunction; ② Children with endocrine or metabolic disorders, autoimmune diseases, or other conditions that could impact bone metabolism; ③ A history of drug poisoning; ④ Secondary obesity resulting from other factors was also excluded. A total of 271 subjects met the inclusion criteria. The criteria for the assessment of pubertal onset were as follows: girls with breast development at or above Tanner II stage and boys with testicular volume of 4ml or greater were considered to have initiated pubertal development [11]. Obese children were categorized into two groups: the puberty development group and the puberty undeveloped group, based on the presence or absence of adolescent development. The study protocol was approved by the Institutional Review Board of Beijing Jishuitan Hospital, Capital Medical University (No.201808-03). Informed consent was obtained from all participants and their legal guardians prior to enrollment.
1.2. Methods
1.2.1. Physical Examination and Body Composition Assessment
The physical examination, body composition, and bone density measurements for all subjects were independently conducted by the same surveyor, with quality control measures implemented each time to calibrate the precision and accuracy of the instruments. Subjects were dressed in light clothing, and their height (in centimeters) and weight (in kilograms) were measured, with the final value being the average of two measurements. The Body Mass Index (BMI) was calculated as weight (in kilograms) divided by the square of height (in meters). Bioelectrical Impedance Analysis (BIA) was employed to measure body composition. The device used was the Sihai Huachen H-Key350 eight-electrode BIA detector. Subjects, in a fasting state (ensuring they had emptied their bladders and refrained from drinking water for 30 minutes prior to measurement), were dressed in light clothing, removed all metallic objects and accessories, stood barefoot on the measurement platform, and made full contact with the electrodes before the measurements were taken. The results for body fat, visceral fat, muscle mass, body fat percentage, and waist-hip ratio were recorded. The Body Fat Index was defined as body fat (in kilograms) divided by the square of height (in meters). The Muscle Mass Index was defined as muscle mass (in kilograms) divided by the square of height (in meters). The Fat-Free Body Mass Index was defined as fat-free weight (in kilograms) divided by the square of height (in meters). The physical examination was performed by a pediatric endocrinologist who had undergone uniform training. This examination was designed to assess pubertal development, which included the evaluation of female breast development and male testicular volume. Breast development was assessed through visual examination and palpation, following the Tanner staging criteria. Testicular volume was determined by palpation and comparison with the Prader testicular meter. In cases where there was a difference in testicular size between the two sides, the larger value was recorded. If the stages of breast development on both sides were different, the more mature side was documented.
1.2.2. Determination of Fasting Insulin and Fasting Blood Glucose
After a 12-hour fast, 5 ml of venous blood was collected and sent to the Laboratory of Beijing Jishuitan Hospital, Capital Medical University, for testing. Fasting insulin (FIN) was measured using the chemiluminescence method, while blood glucose was detected via the glucose oxidase method. The Hitachi 7600 automatic biochemical analyzer was the instrument used for these measurements. The Homeostasis Model Assessment of Insulin Resistance (HOMA-IR) was calculated using the formula: HOMA-IR = (fasting blood glucose [mmol/L] × fasting insulin [uU/ml])/22.5.
1.2.3. The Questionnaire Survey Was Administered by a Professional Pediatrician
Prior to the commencement of the project, the survey was completed with the assistance of the subjects' guardians, under the guidance of the pediatrician. Once the questionnaires were collected, they were entered into a database by two individuals to ensure consistency and logical accuracy. The questionnaire covered a range of topics, including general population characteristics such as date of birth, gender, and birth weight, as well as lifestyle and dietary factors. These factors included nightly sleep duration, engagement in moderate-intensity physical activity, daily sitting time, postnatal feeding methods, and dietary habits such as meat consumption, intake of sugary beverages, and consumption of fried foods in the past year.
1.2.4. Relevant Indicators and Definitions of Lifestyle Behaviors and Dietary Intake
The following definitions were used to categorize the subjects' lifestyle behaviors and dietary intake:① Physical Activity Level: More Exercise: Defined as engaging in moderate-intensity exercise for a total of 180 minutes or more per week. Less Exercise: Defined as engaging in moderate-intensity exercise for less than 180 minutes per week. ② Meat Consumption: More Meat Intake: Defined as consuming meat 5 times or more per week. Less Meat Intake: Defined as consuming meat less than 5 times per week. ③ Sugary Drink Consumption: More Sugary Drink Intake: Defined as consuming sugary drinks 3 times or more per week. Less Sugary Drink Intake: Defined as consuming sugary drinks less than 3 times per week. ④ Fried Food Consumption: More Fried Food Intake: Defined as consuming fried foods 3 times or more per week. Less Fried Food Intake: Defined as consuming fried foods less than 3 times per week. These definitions were used to classify the subjects' behaviors and dietary habits for the purpose of the study.
1.3. Statistical Analysis
For the statistical analysis, version 4.0.3 of the statistical software was utilized. The Shapiro-Wilk normality test was applied to assess the normality of the sample data for continuous variables. If the data conformed to a normal distribution, the mean and standard deviation were used to represent the data, and the independent samples T-test was employed to compare between two groups. In cases where the data did not conform to a normal distribution, the median along with the 25th and 75th percentiles were used, and the Wilcoxon rank-sum test was applied for group comparisons. Categorical variables were described using frequency counts, and group comparisons were made using the Chi-square test or Fisher's exact test, depending on the data distribution and sample size. To screen independent variables, the stepwise AIC (Akaike Information Criterion) function was used in a regression analysis. For binary logistic regression analysis, the glm function was employed. When the independent variable was a categorical variable, the reference group was the group with the minimum value. When the independent variable was continuous, it was directly included in the binary logistic regression model. A difference was considered statistically significant if the P-value was less than 0.05.
2. Result
2.1. Relationship Between Puberty Development and Body Composition and Insulin Resistance in Obese Children
The study included a total of 217 participants. Among them, there were 152 male students, with 83 (54.6%) in the adolescent undeveloped group and 69 (45.4%) in the adolescent developed group. There were 119 female students, with 30 (25.2%) in the adolescent undeveloped group and 89 (74.8%) in the adolescent developed group. Among the male students: Age, height, weight, BMI, BMI z-score, body fat, muscle mass, muscle mass index, fat-free weight, fat-free body mass index, waist-hip ratio, fasting insulin, and insulin resistance index were all higher in the adolescent developed group compared to the adolescent undeveloped group. These differences were statistically significant (P < 0.05). Among the female students: Age, height, weight, BMI, BMI z-score, body fat, muscle mass, fat-free weight, fat-free body mass index, waist-hip ratio, fasting insulin, fasting blood glucose, and insulin resistance index were all higher in the adolescent developed group compared to the adolescent undeveloped group. These differences were also statistically significant (P < 0.05). These results are detailed in Table 1. After excluding the collinearity factor and adjusting for the confounding effects of age and height, BMI was identified as an independent influencing factor of adolescent development among girls (P < 0.05), as shown in Table 2.
Table 1.
Comparison between the Adolescent Undeveloped and Developing Groups of Obese School-Age Children.
Table 1.
Comparison between the Adolescent Undeveloped and Developing Groups of Obese School-Age Children.
| Factors | Male | Female | ||||||
|---|---|---|---|---|---|---|---|---|
| Puberty Undeveloped (n = 83) | Puberty Development (n = 69) | statistic | p | Puberty Undeveloped (n = 30) | Puberty Development (n = 89) | Statistic | p | |
| Age (year) | 9 (7.81,9.88) | 10.92 (9.5,12) | -6.029 | <0.001 | 7.66 (6.94,8.46) | 9.25 (8,10.2) | -4.580 | <0.001 |
| Weight (Kg) | 50.95 (41.85,58.8) | 64.7 (53.2,73.1) | -5.732 | <0.001 | 36.55 (32.5,44.3) | 48.4 (39.5,60) | -4.079 | <0.001 |
| Height (cm) | 141.63 ± 10.57 | 154.29 ± 9.28 | -7.765 | <0.001 | 132.42 ± 9.37 | 143.92 ± 11.68 | -5.445 | <0.001 |
| BMI | 24.4 (22.48,27.07) | 26.3 (24.1,29) | -3.109 | 0.002 | 21.77 (20.26,23.78) | 23.3 (20.9,25.86) | -2.111 | 0.035 |
| BMI z-score | -0.11 (-0.61,0.58) | 0.38 (-0.19,1.09) | -3.109 | 0.002 | -0.79 (-1.18,-0.27) | -0.39 (-1.02,0.27) | -2.111 | 0.035 |
| Body fat (Kg) | 19.74 (15.25,25.18) | 23.65 (19.4,29.13) | -3.034 | 0.002 | 12.5 (11.2,15.28) | 17.39 (13.15,21.4) | -2.979 | 0.003 |
| Body fat index(kg/m2) | 9.6 (7.69,12) | 10.1 (8.21,11.78) | -0.731 | 0.465 | 7.56 (6.6,8.74) | 8.21 (6.62,9.91) | -1.195 | 0.232 |
| Muscle mass (Kg) | 29.2 (25.17,32.52) | 35.65 (32.5,42.1) | -6.682 | <0.001 | 23 (20.9,26.5) | 28.55 (23.97,34.6) | -3.900 | <0.001 |
| Muscle mass index(kg/m2) | 14.4 (13.58,15.03) | 15.13 (14.59,16.32) | -4.733 | <0.001 | 13.44 (13.09,14.14) | 13.85 (13.25,15.38) | -1.948 | 0.051 |
| Fat-free weight (Kg) | 31 (26.5,34.52) | 38.25 (35,44.97) | -6.930 | <0.001 | 24.5 (22.2,28) | 30.4 (25.55,36.77) | -3.945 | <0.001 |
| Fat-free weight index(kg/m2) | 15.22 (14.42,15.92) | 16.13 (15.54,17.35) | -5.360 | <0.001 | 14.23 (13.81,14.91) | 14.72 (14.07,16.33) | -2.139 | 0.032 |
| Body fat percentage(%) | 38.65 (34.15,42.9) | 38.3 (33.98,41.3) | 0.500 | 0.617 | 34.4 (31.8,37.2) | 35.2 (31.63,39.75) | -0.602 | 0.547 |
| Waist-hip ratio | 0.84 (0.79,0.89) | 0.86 (0.82,0.9) | -2.102 | 0.036 | 0.77 (0.76,0.83) | 0.82 (0.78,0.87) | -2.346 | 0.019 |
| Fasting insulin (uU/mL) | 17.1 (12.35,27.4) | 23.5 (14,33.7) | -2.309 | 0.021 | 13.55 (10.07,21.12) | 18.7 (12.2,29.9) | -2.370 | 0.018 |
| Fastingblood sugar (mmol/L) | 5.03 ± 0.33 | 5 ± 0.47 | 0.435 | 0.664 | 4.8 (4.5,5.1) | 5 (4.8,5.3) | -2.632 | 0.008 |
| HOMA-IR | 3.9 (2.69,5.89) | 5.49 (3.31,7.6) | -2.261 | 0.024 | 2.96 (2.2,4.65) | 4.24 (2.77,6.93) | -2.594 | 0.009 |
Table 2.
Multiple Regression Analysis of Adolescent Development Related Factors of Obese Children Aged 6-12.
Table 2.
Multiple Regression Analysis of Adolescent Development Related Factors of Obese Children Aged 6-12.
| Factors | Male | Female | |||||||
|---|---|---|---|---|---|---|---|---|---|
| B | SE | z | p | B | SE | z | p | ||
| Puberty development | constant | -19.097 | 4.361 | -4.379 | <0.001 | -9.457 | 5.719 | -1.654 | 0.098 |
| Age | 0.150 | 0.169 | 0.885 | 0.376 | 0.465 | 0.290 | 1.602 | 0.109 | |
| Height | 0.133 | 0.038 | 3.523 | <0.001 | 0.091 | 0.046 | 1.964 | 0.050 | |
| BMI | -0.060 | 0.090 | -0.674 | 0.5 | 0.055 | 0.132 | 0.416 | 0.021 | |
| Muscle mass index | 0.392 | 0.219 | 1.787 | 0.074 | 0.110 | 0.408 | 0.269 | 0.788 | |
| Waist-hip ratio | -7.639 | 4.119 | -1.855 | 0.064 | -6.968 | 5.906 | -1.180 | 0.238 | |
| Fasting insulin | -0.002 | 0.013 | -0.121 | 0.903 | -0.018 | 0.017 | -1.085 | 0.278 | |
2.2. The Impact of Lifestyle, Diet, and Other Factors on the Development of Obese Adolescents in School-Age Children
In Table 3, it is observed that the birth weight of boys in the adolescent development group is significantly higher than that of boys in the adolescent undeveloped group (P < 0.05). Additionally, the consumption of sugary drinks and fried foods is notably higher in the adolescent development group compared to the adolescent undeveloped group (P < 0.05). Conversely, the engagement in moderate-intensity exercise is significantly lower in the adolescent development group than in the adolescent undeveloped group (P < 0.05). For adolescent girls, the developmental group exhibits a higher daily sitting time and a greater intake of sugary drinks and fried foods compared to the undeveloped group, with these differences being statistically significant (P < 0.05). Multiple regression analysis indicates a positive correlation between the intake of fried foods and the puberty development of both boys and girls, establishing it as an independent influencing factor of puberty development (P < 0.05) (Table 4 and Table 5).
Table 3.
Comparison of Dietary and Lifestyle Behaviors Between the Adolescent Undeveloped Group and the Adolescent Developing Group Among Obese Children.
Table 3.
Comparison of Dietary and Lifestyle Behaviors Between the Adolescent Undeveloped Group and the Adolescent Developing Group Among Obese Children.
| Factors | Male | Female | ||||
|---|---|---|---|---|---|---|
| Puberty Undeveloped (n = 83) | Puberty Development (n = 69) | p | Puberty Undeveloped (n = 30) | Puberty Development (n = 89) | p | |
| Night sleep time (h) | 9 (8.5,9.2) | 9 (8.5,9.2) | 0.613 | 9 (8.5,9.4) | 8.8 (8.3,9) | 0.462 |
| Amount of exercise | 0.003 | 0.82 | ||||
| Less | 53 (63.9%) | 59 (85.5%) | 23 (76.7%) | 70 (78.7%) | ||
| More | 30 (36.1%) | 10 (14.5%) | 7 (23.3%) | 19 (21.3%) | ||
| Sit-in time (min) | 60 (30,120) | 60 (40,120) | 0.22 | 30 (20,65) | 60 (40,120) | 0.003 |
| Meat intake | 0.575 | 0.881 | ||||
| Less | 23 (27.7%) | 22 (31.9%) | 11 (36.7%) | 34 (38.2%) | ||
| More | 60 (72.3%) | 47 (68.1%) | 19 (63.3%) | 55 (61.8%) | ||
| Sugary beverage intake | <0.001 | 0.001 | ||||
| Less | 72 (86.7%) | 36 (52.2%) | 26 (86.7%) | 46 (51.7%) | ||
| More | 11 (13.3%) | 33 (47.8%) | 4 (13.3%) | 43 (48.3%) | ||
| Fried food intake | <0.001 | <0.001 | ||||
| Less | 80 (96.4%) | 36 (52.2%) | 28 (93.3%) | 40 (44.9%) | ||
| More | 3 (3.6%) | 33 (47.8%) | 2 (6.7%) | 49 (55.1%) | ||
| Birth weight (g) | 3408 ± 507.5 | 3574.1 ± 509.9 | 0.048 | 3454.8 ± 529.5 | 3442.8 ± 455.2 | 0.907 |
Table 4.
Multiple Regression Analysis of Adolescent Development, Lifestyle Behaviors, and Diet Among Boys.
Table 4.
Multiple Regression Analysis of Adolescent Development, Lifestyle Behaviors, and Diet Among Boys.
| Factors | B | SE | z | p | |
|---|---|---|---|---|---|
| Puberty development | constant | -4.662 | 1.709 | -2.728 | 0.006 |
| Amount of exercise | -0.040 | 0.876 | -0.046 | 0.964 | |
| Sugary beverage intake | 0.267 | 0.579 | 0.461 | 0.645 | |
| Fried food intake | 2.981 | 0.765 | 3.898 | <0.001 | |
| Birth weight | 0.000 | 0.000 | 0.903 | 0.367 |
Table 5.
Multiple Regression Analysis of Adolescent Development, Lifestyle Behaviors, and Diet Among Girls.
Table 5.
Multiple Regression Analysis of Adolescent Development, Lifestyle Behaviors, and Diet Among Girls.
| Factors | B | SE | z | p | |
|---|---|---|---|---|---|
| Puberty development | constant | -2.914 | 1.000 | -2.914 | 0.004 |
| Sit-in time | 0.008 | 0.005 | 1.656 | 0.098 | |
| Sugary beverage intake | 0.229 | 0.733 | 0.312 | 0.755 | |
| Fried food intake | 2.535 | 0.891 | 2.845 | 0.004 |
3. Discussion
Previous research has demonstrated that fat is a vital component for sustaining life and metabolic processes. However, an excess or abnormal accumulation of fat can be detrimental to human health. Obesity is associated not only with an increased risk of various metabolic complications but also impacts adolescent development. In 1993, Frisch, after analyzing the long-term follow-up data of 181 normal children, introduced the concept of "critical weight." This concept suggests that women require a certain amount of fat reserves to initiate puberty, and body fat is also an essential element for maintaining fertility [12]. Numerous population-based studies have identified a correlation between high BMI in girls and their adolescent development. Our study's findings indicate that among obese school-age girls, those in the adolescent development group exhibit higher weight, BMI, and BMI-z scores compared to those in the adolescent undeveloped group. BMI is an independent influencing factor in the adolescent development of obese school-age girls. Previous research has also substantiated the link between obesity and female puberty development. A meta-analysis of 30 studies reveals that from 1977 to 2013, the age of female breast development has been occurring three months earlier every decade, a trend that coincides with the increasing prevalence of obesity year by year [13]. A cross-sectional study of girls in China indicates that those who are overweight or obese tend to experience earlier development of breasts and pubic hair compared to their peers with a healthy weight [14,15].
The critical BMI value is highly specific for diagnosing obesity, meaning it can accurately identify those who are obese. However, it has low sensitivity for detecting obesity, which means it may miss some individuals who are actually obese. BMI does not provide information about the proportions of various components of body weight, such as body fat, muscle mass, fat-free weight, and body fat distribution [16]. BMI, body fat, and muscle mass undergo changes as adolescents develop, and these changes are accompanied by gender-specific differences. Our research has shown that among obese boys in the adolescent development group, there are higher levels of body fat, muscle mass index, fat-free body mass index, and waist-hip ratio compared to those in the adolescent undeveloped group. In contrast, for obese girls, we observed higher levels of body fat, fat-free body mass index, and waist-hip ratio. Xu et al. [17] posited that there are discernible gender differences in BMI, waist circumference, and body fat percentage as puberty progresses. Specifically, the body fat percentage in girls tends to increase with the onset of puberty, a pattern that does not hold true for boys. The fat accumulation in girls may largely account for the post-puberty increase in BMI. In contrast, the rise in BMI among boys is more likely due to the accumulation of fat-free mass. An additional study conducted with obese children in Beijing, China, demonstrated that female BMI and body fat percentage were positively correlated with early puberty development. In the case of boys, the early puberty development group had a higher BMI but a lower body fat percentage [9].
Currently, the mechanism by which obesity affects adolescent development remains unclear. Obesity is associated with a range of changes in metabolic cytokines and hormones, including leptin, gastrin, insulin, and certain central lipids. The alterations in these metabolites may impact the Hypothalamus-Pituitary-Gonad (HPG) axis and play a role in the regulation of adolescent development. Hypothalamic Kisspeptin neurons are thought to be the key nexus between metabolism and adolescent development. Kisspeptin, secreted by the kiss1 gene, directly acts on gonadotropin-releasing hormone (GnRH) neurons, thereby activating the onset of puberty. The increase in adipose tissue in obese children stimulates an increase in leptin secretion, which in turn continues to stimulate the expression of the kiss1 gene. This process may accelerate the onset of puberty. Leptin and Kiss1 neurons are interconnected in central brain regions and may overlap, suggesting that the accumulation of metabolic hormones in the hypothalamus could be a significant factor in explaining the link between childhood obesity and central precocious puberty. Additionally, persistent hyperglycemia and insulin resistance in obese individuals have been shown to play a role in the function of the HPG axis [18]. Our study also suggests that the fasting insulin levels and insulin resistance index are higher in the adolescent development group compared to the adolescent undeveloped group. Hyperinsulinemia can lead to a decrease in the levels of sex hormone-binding globulin (SHBG), thereby increasing the bioavailability of sex hormones and stimulating the secretion of androgens in the adrenal glands and ovaries. Previous studies have indicated that increasing insulin sensitivity can reduce androgen levels in the adrenal glands and ovaries, and may delay the progression of puberty [19]. In addition, epigenetic changes, such as polymorphisms in the MKRN3 and LIN28B genes, as well as prenatal and postnatal environmental factors, intestinal microbiota, and endocrine disruptors, are also associated with the adolescent development of obese children and adolescents [20].
Genetic predispositions, climate, diet, nutritional status, socio-economic factors, physical activity, and health status all impact the development of adolescence, and these same factors also contribute to the development of obesity. In contemporary society, which is rich in material resources, children and adolescents face multiple pressures from academics, parental expectations, and social interactions. These pressures can disrupt cognitive functions such as executive function and self-regulation, and are often alleviated through behaviors such as overeating, consuming high-calorie foods, increasing sedentary activities like watching online videos, reducing physical activity, and shortening sleep duration. Moreover, the widespread availability of fast food in the modern era makes it nearly inevitable that there will be a mismatch between calorie intake and energy expenditure, leading to fat accumulation. This study demonstrates that compared to obese boys who have not yet entered adolescence, those in the adolescent development group engage in less exercise and consume more sugary drinks and fried foods. In the case of obese girls, the adolescent development group also reports higher daily sitting times and greater intake of sugary drinks and fried foods than the adolescent undeveloped group. Excessive consumption of fried foods is associated with the development of adolescence in obese children. Research from an Italian team concluded that the rising incidence of central precocious puberty may be related to a significant increase in BMI, the increased use of electronic products, and the growing psychological stress [21]. A meta-analysis by Nguyen et al. indicates that a diet high in fiber and monounsaturated fatty acids is associated with later menarche, while a diet high in animal protein and polyunsaturated fats is associated with earlier menarche. The habitual consumption of sugary soft drinks is positively correlated with the risk of early adolescence [22]. A meta-analysis revealed that a diet rich in fiber and monounsaturated fatty acids is associated with later menarche, while a diet high in animal protein and polyunsaturated fats is linked to earlier menarche. Additionally, the regular consumption of sugary soft drinks is positively correlated with the risk of early adolescence [23]. While the pivotal role of nutritional status in the activation of adolescence has been substantiated by numerous studies, the neuroendocrine mechanisms underlying this relationship remain enigmatic. The potential mechanisms by which a high-fat diet may promote adolescent development could include the following: Activation of gonadotropin-releasing hormone (GnRH) through hypothalamic microglia. The interplay of intestinal microflora and hormonal actions. Overexpression of the p53 gene via the Lin28/let-7 system [24].
This study does have several limitations: (1) The subjects are regionally restricted, and the sample size is small, which makes it impossible to compare sexual development with normal-weight children of the same age and sex. (2) The content of the questionnaire in this study is limited, and there may be some memory bias when filling it out. However, the questionnaire is designed based on a large-sample epidemiological survey of children's chronic diseases in China, and its reliability is ensured. (3) The confounding factors controlled in this study are limited, and the research results need to be verified by high-quality prospective cohort studies. Despite these limitations, our research provides some reliable cross-sectional data on the development of adolescence in 6-12-year-old obese children. In the follow-up of this study, it is necessary to further expand the scope of investigation, increase the sample size, and enrich the research content to make the research results more scientific and reliable.
4. Conclusions
In conclusion, 6-12-year-old obese children who have entered adolescence exhibit higher levels of body fat, fat-free body mass index, and waist-hip ratio. Moreover, BMI is positively correlated with the development of adolescence in obese girls. The excessive consumption of fried foods may promote the development of adolescence in obese children. The interplay of genetics, epigenetics, metabolism, nutrition, and hormones influences the onset and progression of puberty. Further research is needed to elucidate the mechanisms of these factors, which is crucial for developing strategies to prevent precocious puberty in obese children.
Funding
The special fund of the Pediatric Medical Coordinated Development Center of Beijing Hospital Authority No.
XTZD20180401.
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