This study investigated the polyphenolic composition and antioxidant potential of Medjool date tissues (flesh and seeds) from two plantations in the Jordan Valley, using chromatographic (HPLC/DAD) and spectrophotometric methods (Folin-Ciocalteau and DPPH assays). Significant levels of phenolic compounds, including gallic acid, ferulic acid, tannins, and flavonoids, were identified. The analysis revealed that polyphenol concentrations in date pulp varied depending on plantation practices and environmental conditions. Additionally, date seed extracts exhibited substantially higher antiradical efficiency compared to flesh extracts. These findings highlight the excellent nutritional and health-promoting properties of Medjool dates. Furthermore, the research emphasizes the potential of date seeds and downgraded fruits for applications in nutraceuticals and functional foods, supporting sustainability and circular economy principles. The study underscores the significance of Medjool dates as a premium cultivar for health-oriented products and suggests strategies to enhance Palestinian date exports despite ongoing logistical and geopolitical challenges.