Novel Biomarkers of Grade I Diastolic Dysfunction in Type 2 Diabetes Patients with Metabolic Dysfunction-Associated Steatotic Liver Disease

Adina Braha; Bogdan Timar; Viviana Ivan; Monica Micloș Balica; Larisa Dăniluc; Romulus Timar

doi:10.20944/preprints202409.1312.v1

Submitted:

17 September 2024

Posted:

17 September 2024

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Abstract

Background/aims: Prior research identified a significant association between heart disease and metabolic dysfunction-associated liver steatohepatitis (MASLD), but the mechanisms are unclear. This study aims to search predictive biomarkers for grade I left ventricular diastolic dysfunction (LVDD), an early stage of heart failure (HF), in type 2 diabetes mellitus (T2DM) patients. Methods: This single-center, prospective observational study screened 73 T2DM patients for grade 1 LVDD and MASLD using 2D echocardiography, tissue analysis, spectral color Doppler, and Fibromax. Results: The study analyzed 50 patients (mean age 58±11.3 years) with a median diabetes duration of 7 years, abdominal obesity (mean BMI 34.4±5.9 kg/m2), and a mean HbA1c of 7.9±1.5%. The prevalence of grade I LVDD, fibrosis, mild steatosis, moderate to severe liver steatosis, mild MASLD, and moderate MASLD was 54%, 44%, 14%, 80%, 43%, and 34%, respectively. Regression analysis revealed that grade 1 LVDD was positively associated with age, Fibrotest, ɑ2-macroglobulin, epicardiac adipose tissue (EAT), and negatively associated with lateral s`, E wave, E/e`, E/A, medium E`, and septal e` (p< 0.05 for all). ɑ2-macroglobulin > 1.92 g/L (AUROC= 0.782, sensitivity 70.4%, specificity 81.2%) and fibrotest score > 0.11 (AUROC 0.766, sensitivity 92.6%, specificity 56.2%) were significant predictors of grade I LVDD. Conclusion: HF is a multifaceted disorder associated with multisystem organ damage, including nonalcoholic MASLD. Although the underlying mechanisms remain unclear, innovative non-invasive biomarkers, such as ɑ2-macroglobulin or fibrotest, could concurrently indicate liver stiffness and the likelihood of grade I LVDD, an early, asymptomatic HF stage in T2DM patients.

Keywords:

predictive biomarkers

;

left ventricular diastolic dysfunction

;

metabolic dysfunction-associated liver steatohepatitis

;

type 2 diabetes mellitus

Subject:

Medicine and Pharmacology - Endocrinology and Metabolism

1. Introduction

Diabetes mellitus (DM) has emerged as a significant global public health challenge, ranking as the third leading cause of death worldwide. Recent statistical data reveal a troubling rise in the prevalence of diabetes [1]. The growth acceleration persisted after COVID-19, as suboptimal management became a potent indicator of more severe consequences in SARS-CoV-2 infections among individuals with type 2 DM (T2DM) [2]. In 2021, Romania documented roughly 1.5 million diagnosed diabetes cases, consistent with global trends. Additionally, it was observed that one out of every twelve adults in Romania had diabetes [3]. DM has numerous chronic complications, comorbidities including liver steatosis and fibrosis [4], cataracts, glaucoma [5], heart failure (HF) [6,7], psychiatric disorders [7,8,9,10], and a high risk of solid malignancies [11,12,13,14,15]. The biological consequences of this issue are significant, as evidenced by a decrease in life expectancy and health expectancy. This is primarily due to the negative effect on the quality of life caused by both acute and chronic complications. Between 2014 and 2021, the most common complications of DM in Romania were due to strokes and polyneuropathies, followed by retinopathies. The least common complications were acute myocardial infarction [16]. However, data about the prevalence of HF in Romanian patients is lacking.

In recent years, the guidelines of the professional associations responsible for diabetes care have recommended the personalization of T2DM therapy according to the presence of comorbidities such as atherosclerotic cardiovascular disease, HF, or chronic kidney disease, in which the indicated molecules are from the class of glucose co-transporter-2 inhibitors (iSGLT2) or glucagon-like-peptide-1 (GLP-1) receptors agonist with clinical evidence in reducing the risk of these complications [17]. However, according to the new guidelines, only a few patients are currently undergoing treatment. One reason may be the underdiagnosis of these comorbidities, such as HF.

HF is a medical disorder characterized by diverse underlying etiologies and pathophysiological processes [18]. HF, which affects over 56 million individuals globally, presents a considerable burden on health worldwide, resulting in decreased quality of life, recurrent hospitalizations, elevated healthcare expenditures, and a substantial incidence of premature death [18,19,20]. HF has gained considerable attention in diabetology in recent years, primarily due to the critical role of iSGLT2 in preventing and treating cardiovascular disease (CVC) and HF. It is widely recognized that HF is a common complication associated with diabetes [21].

HF is a heterogeneous syndrome composed of various clinical entities and differing stages. It can be classified into three primary categories, including HF with reduced ejection fraction (HFrEF), which is characterized by a left ventricle ejection fraction (LVEF) of 40% or less; HF with mildly reduced ejection fraction (HFmrEF), where the LVEF ranges from 41% to 49%; and HF with preserved ejection fraction (HFpEF), which is defined by an LVEF of 50% or higher. Furthermore, distinct phenotypes of ventricular dysfunction in systole (left ventricular systolic dysfunction) and diastole (left ventricular diastolic dysfunction (LVDD) can be identified through echocardiography [22]. The following text describes phenotypes that display ventricular dysfunction without any noticeable symptoms of HF [23]. Among the categories mentioned, LVDD and HFpEF are currently considered the most prevalent phenotypes of HF in T2DM. However, there is no universally accepted estimate of the precise prevalence of the various HF subtypes [24,25].

A comprehensive analysis of the occurrence and likelihood of HF diagnosis and outlook in T2DM patients unveiled that the occurrence of LVDD enhanced by up to 43%, which fluctuates depending on the category. Grade I LVDD is more widespread, accounting for up to 40% of the cases [26].

It is essential to accurately diagnose and identify individuals at risk for HF, as the pathophysiology, treatment, and prognosis vary depending on the specific subtype of HF. A timely diagnosis is crucial in this regard [27].

A systematic review recently carried out has revealed a strong connection between nonalcoholic fatty liver disease (NAFLD) and its link to LVDD, indicating that NAFLD is a multisystemic disorder with close ties to CVDs. Globally, NAFLD is a widespread metabolic disease affecting approximately a quarter of the population [28], an independent risk factor for CVDs [29,30]. A recently introduced definition is a metabolic dysfunction-associated steatotic liver disease (MASLD), formerly known as NAFLD [31]. The implications of this alteration on cardiac structural and functional abnormalities remain unclear [32,33,34].

The recent epidemiological data indicates that MASLD is linked to not only accelerated coronary artery disease but also several other detrimental effects on the heart, such as an elevated risk of LVDD and hypertrophy, as well as cardiac valvular calcification and arrhythmias, particularly persistent atrial fibrillation [35,36,37,38,39]. Furthermore, a comprehensive meta-analysis of 11 longitudinal cohort studies, which incorporated data from over 11 million middle-aged individuals from recent times, revealed that MASLD significantly increases the long-term risk of developing HF by 1.5-fold, regardless of the presence of hypertension, T2DM, and other common cardiometabolic risk factors [40].

However, it is crucial to establish whether MASLD is merely an association in patients with LVDD owing to shared metabolic risk factors or whether it is an independent risk factor for LVDD. This issue necessitates in-depth investigation through high-quality randomized clinical trials [41].

The study analyzed the relationship between the right and left ventricular function, epicardiac adipose tissue thickness (EAT), liver steatosis, and fibrosis evaluated through fibromax tests.

2. Materials and Methods

2.1. Study Design and Patients

In this single-center, cross-sectional study, patients were prospectively enrolled between October 10, 2022, and June 01, 2024, from the Diabetes Clinic and outpatient Diabetes Center of the University Emergency County Hospital Pius Brînzeu Timisoara, Romania. The hospital ethics committee approved this research (332/October 10, 2022) as it met the requirements of the Helsinki Declaration (version 2013), respecting the confidentiality of patient data, according to the General Data Protection Regulation (GDPR) Compliance. All participants signed a written informed consent form before enrollment in the study.

Patients were eligible for study enrollment if the following criteria were present: T2DM patients over 18 years of age, without cardiac valvulopathies or significant cardiac disease, without chronic viral or alcoholic hepatitis, with no alcohol misuse, left ventricular ejection fraction (LVEF) > 40%, and an estimated glomerular filtration rate (eGFR) >30 mL/min. The exclusion criteria were as follows: LVEF< 40% and significant structural heart disease, ongoing or planned pregnancy, lactation, other types than T2DM, eGFR < 30 mL/min, underweight with a body mass index (BMI) < 23 kg/m², history of pancreatitis, or refusal to participate in this clinical trial.

2.2. Laboratory Tests and Clinical Examinations

Under fasting conditions (minimum 12 hours of food rest), the patients underwent routine laboratory tests (glycated hemoglobin (HbA1c), high-density lipoprotein cholesterol (HDLc), low-density lipoprotein cholesterol (LDLc), estimated glomerular filtration rate (eGFR), urinary albumin/creatinine ratio (UACr), total bilirubin (TB), alkaline phosphatase (FAL)), and fibromax, with the prior signing of a responsibility assumption form regarding compliance with the pre-analytical conditions established by the external lab and the provision of correct height and weight data. Fibromax is a medical laboratory analysis tool developed by BioPredictive, in which the following five non-invasive tests are combined: FIBROTEST (degree of liver fibrosis), ACTITEST (necroinflammatory activity), STEATOTEST (degree of hepatic steatosis), NASHTEST (presence of nonalcoholic steatohepatitis in patients with dyslipidemia, insulin resistance, diabetes, or obesity), and ASHTEST (degree of liver damage in patients with excessive alcohol consumption). The Fibromax score for evaluating the degree of liver fibrosis was obtained using a calculation algorithm that combined the results of medical laboratory analyses with the patient's age, sex, weight, and height. Medical laboratory analyses determined for Fibromax included ɑ2 macroglobulin, apolipoprotein A1, total bilirubin, haptoglobin, gamma-glutamyl-transferase (GGT), alanine aminotransferase (ALT), aspartate aminotransferase (AST), glycemia, total cholesterol, and triglycerides. Interpretation of Fibromax results was obtained from medical laboratory analyses entered on the BioPredictive website, where a report was generated with the scores of fibrosis, necroinflammatory activity, hepatic steatosis, nonalcoholic steatohepatitis, and alcoholic steatohepatitis.

We assessed the anthropometric characteristics of the patients by calculating their body mass index (BMI) and visceral adipose tissue (VAT). The abdominal waist circumference was measured with a measuring tape midway between the lower rib and iliac crest, and the proximal thigh circumference (proximal thigh C) was measured 15 cm proximal to the superior pole of the patella. The VAT was calculated using the formula VAT (women) = 2.15 × waist circumference - 3.63 × proximal thigh circumference + 1.46 × age + 6.22 × BMI - 92.713, respectively VAT (men)= 6 × waist circumference - 4.41 × proximal thigh circumference + 1.19 × age - 213.65 [42].

2.3. Echocardiac Ultrasound Protocol

Two expert cardiologists evaluated cardiac function and epicardiac adipose tissue (EAT) by performing 2D echocardiography and tissue and spectral color Doppler. The echocardiographic parameters studied were: left atrium (LA) diameter (mm) in the parasternal incidence, long-axis view, left ventricle ejection fraction (LVEF), EAT measured in the subcostal view, parasternal long axis as the hypoechoic distance between the liver and the right ventricular wall at the end of systole, tricuspid annular plane systolic excursion (TAPSE), global longitudinal strain (GLS), and the peak velocities of the early (E) and late (A) phases of mitral valve (MV) inflow during diastole (cm/s). We calculated the E/A ratio; E-wave deceleration time (DTE); MV a`, s`, e` lateral wall velocities; MV a`, s`, e` septum velocities; calculated average E/e` ratio; ascending aorta (AO);

The 10-year primary risk of atherosclerotic cardiovascular disease (ASCVD) was estimated using the Pooled Cohort Equation (PCE) calculator. The risk was categorized as low (<5%), borderline (5 to <7.5%), intermediate (≥7.5% to <20%), or high (≥20%) [43,44].

2.4. Definitions and Outcomes

We defined grade 1 LVDD according to the diastolic dysfunction grading algorithm from the guide for evaluating left ventricular diastolic function by echocardiography developed by the American Society of Echocardiography and the European Association of Echocardiography [45], using the following criteria E/A < 0.8, DTE> 200ms, average E/e' ≤8. In the data analysis, we coded the presence of LVDD as 1 and its absence or the presence of other diastolic dysfunction as 0.

MASLD was defined in studied T2DM patients with liver steatosis and at least one of five cardiometabolic risk factors [46].

The FIBROTEST assesses liver fibrosis and is classified as no fibrosis (F0), minimal fibrosis (F1), moderate fibrosis (F2), advanced fibrosis (F3), and severe fibrosis (F4). The liver steatosis assessed with STEATOTEST classified as S0, no steatosis (<5% fat overload), S1, mild steatosis (5-33% fat overload), S2S3 clinically significant moderate to severe steatosis (34-100% fat overload). Compared to a burn, liver inflammation assessed with ACTITEST is classified as A0, A1, A2, or A3, meaning no, minimal, significant, or severe active inflammation. The alcoholic inflammation was verified with ASHTEST from H0 to H3 alcoholic inflammation. NASHTEST assessed the inflammation as an over-reaction of the body to an accumulation of fat in the liver, present in metabolic diseases such as diabetes, overweight, and dyslipidemia and classified as N0, no NASH, N1, mild NASH, N2, moderate NASH, respectively N3, severe NASH. According to the new nomenclature, we present the data as MASLD instead of NASH in the present study of T2DM patients who also present with obesity and dyslipidemia.

The main objective of this study was to evaluate the possible predictors of grade 1 LVDD in patients with T2DM.

2.5. Statistical Analysis

The data were statistically analyzed using MedCalc® Statistical Software version 22.016 (MedCalc Software Ltd, Ostend, Belgium; 2023). To meet the desired statistical constraints for the study outcome, we applied a Shapiro-Wilk test to determine whether the continuous numerical variables were normally distributed. Normal-distributed variables are presented as mean and standard deviation, while non-parametric variables are presented as median and IQR values. The number of individuals in the class and the percentage of the total subgroup represent the qualitative/nominal variables. To assess disparities in central tendency indicators between groups, we utilized t-tests (paired/unpaired) to compare the arithmetic means of parametric variables between two groups and Mann-Whitney-U tests to assess medians.

Additionally, we employed chi-square tests to determine the statistical significance of differences in proportions between groups. We conducted multivariate and logistic regression analyses to evaluate the strength of the relationships between numerical variables. The coefficient of determination (R2) was calculated to verify the proportion of variation in the independent variable that generated the variation in the dependent variable. To verify the statistical significance of the analyzed correlations and, consequently, the possibility of generalizing the association in the population, we used the distribution test of t-values, which considers the value of the correlation coefficient and the size of the studied sample. To evaluate the association of LVDD grade I with the factors included in the analysis measured on a continuous scale, we built univariate, then multivariate logistic regression models, with potential predictors as of the variables mentioned above, respectively outcome as a dichotomous event, grade I LVDD. The variation of the risk of occurrence of the outcome was interpreted by the exponent means of B (Exp (β) coefficient of the regression equation, equivalent to the percentage change in the relative value of the risk relative to an increase with a unit of measure in the predictor scale. With the help of Nagelkerke's pseudo-coefficient of determination, we showed how the regression model built explains the occurrence of the dichotomous event.

To assess the predictive ability of grade I LVDD based on a continuous variable's value, we conducted "Receiver-Operating Characteristics" (ROC) analyses. The predictive performance was measured using sensitivity and specificity. The optimal threshold value for the predictor was determined to be the Youden index. To evaluate the statistical significance of the predictive capability, we compared the area under the ROC (AUROC) curves of the model with the non-discriminant model. For 90% study power, the required sample size for the comparison of the area under the ROC curve with a null hypothesis value was 50 patients based on an α-level of 0.05, two-sided, β-level of 0.10, with 0.760 AUC expected to be found in the study, a null hypothesis AUC of 0.5, and the ratio of sample sizes in negative/positive groups ½.

In our analysis, we determined the 95% confidence interval (CI) and considered a p-value of less than 0.05 statistically significant.

3. Results

The study included 50 patients, 46% (23/50) men. Overall, the group had a mean age of 58±11.3 years, median diabetes duration of 7 (4;15) years, abdominal obesity (mean BMI 34.4±5.9 kg/m², mean waist 116±11 cm), who had a mean HbA1c of 7.9±1.5%. Women tended to have a higher BMI. However, men had more VAT than women (median 55.7 vs. 95.7 cm², p= 0.01). As for lipid profile, women presented higher values of apo A1, TG, and LDLc than men (p< 0.05); all patients were on statin therapy with atorvastatin or rosuvastatin. However, men had lower median GLS values than women (14.2 vs. 17.4%, p= 0.02). The 2D echocardiography and tissue and spectral color Doppler revealed significantly higher values of MV a' lateral wall and septum velocities and ascending AO in men than in women. FIBROTEST, ACTITEST, and ASHTEST results were also significantly higher in men than women. The comparison of general characteristics by gender is presented in Table 1.

The Fibromax scores revealed that 44% had different grades of fibrosis, 14% (7/50) had mild steatosis, 80% (40/50) had moderate to severe liver steatosis, 43% (23/50) had mild MASLD, and 34% (17/50) had moderate MASLD (Figure 1).

Interpretation of the fibromax results as follows: FIBROTEST no fibrosis (F0), mild fibrosis (F1), moderate fibrosis (F2), advanced fibrosis (F3), and severe fibrosis (F4); STEATOTEST S0, no steatosis (<5% fat overload), S1, mild steatosis (5-33% fat overload), S2S3 clinically significant moderate to severe steatosis (34-100% fat overload); ACTITEST A0, no activity, A1, minimal activity, A2, significant activity, and A3, sever activity inflammation; ASHTEST from H0 to H3 alcoholic inflammation; NASHTEST N0, no NASH, N1, mild NASH, N2, moderate NASH, respectively N3, severe NASH.

All patients had normal FEVS, TAPSE, and mean GLS of 16.1±2.5%. 54% (27/50) of the patients had grade I LVDD. Patients with grade I LVDD were older, had higher values in mean VAT (86.2±46.9 cm² vs. 57.7±33.9 cm², p= 0.04), mean EAT (4.6±1.5 mm vs. 3.4±1.6 mm, p= 0.01), median LA diameter (3.9 cm vs. 3.3 cm, p= 0.009), mean ascending AO (2.9 ± 0.3 cm vs. 2.6 ± 0.3 cm, p= 0.005), median DTE (219.5 ms vs. 183 ms, p< 0.001), median average E/e` (7.6 ms vs. 6.7 ms, p<0.0001), lower values in median MV e` septum velocities (7 cm/s vs. 10.5 cm/s, p= 0.0001), and MV e` lateral wall velocities (8 cm/s vs. 12 cm/s, p= 0.0001), compared with patients without LVDD. Notably, patients with grade I LVDD had a significantly higher PCE score of 22.8 % vs. 10.8 %, p= 0.02. Regarding fibromax results, the FIBROTEST score was significantly higher in patients with grade I LVDD than those without, 0.19 vs. 0.11, p= 0.003. After applying the chi-squared test across the severity of different fibromax scores, there were no statistical differences in the frequency of grade I LVDD. ɑ2 macroglobulin was significantly higher in patients with grade I LVDD (2.2 g/L vs. 1.6 g/L, p= 0.002). Table 2 presents the differences between the studied factors by the presence of grade I LVDD.

In regression analysis, the strength of the associations between the numerical variables analyzed was evaluated with the help of regression coefficients: In the case of bivariate regressions, we calculated the coefficient of determination (R²) to verify in what proportion the variation of the independent variable (studied factors) generates the variation of the dependent variable (grade I LVDD). To verify the statistical significance of the analyzed correlations and, consequently, the possibility of generalizing the association in the population, we used the distribution test of t-values that considers the value of the correlation coefficient and the size of the studied sample. Table 3 presents the results of the regression analysis. There were significant direct associations between the outcome (grade I LVDD) and age, VAT, ɑ2 macroglobulin, EAT, TAPSE, LA, DTE, MV s` lateral wall velocities, ascending AO, MV A wave velocity, PCE risk, and FIBROTEST score, respectively negative associations with MV e wave velocity, average E/e,` MV e` septum and lateral wall velocities.

According to the univariate logistic regression model presented in Table 4, age is a statistically significant predictive factor (Exp (β) = 1.25, p = 0.001). This result suggests that for every 1-year-old increase, the risk of grade 1 LVDD increases by 25%. For every 1 cm² VAT and every 1 mm EAT increase, the risk of grade 1 LVDD increases by 1% (p= 0.04), respectively, by 94% (p= 0.03). Similarly, for every 1% increase in PCE risk, the risk of grade 1 LVDD increases by 12%. Increases in MV s` lateral wall velocities, E wave velocities, e` septum, and lateral wall velocities decrease the likelihood of grade I LVDD. Increases in MV A wave velocities, DTE, average E/e`, ɑ2 macroglobulin, and FIBROTEST score increase the likelihood of grade I LVDD.

A value higher than 1.92 g/L in ɑ2 macroglobulin represented a statistically significant predictive value (AUROC curve = 0.782, sensitivity 70.4 %, specificity 81.2 %, p= 0.001) for grade 1 LVDD (Figure 2a). Similarly, FIBROTEST scores above 0.11 predict the LVDD grade 1 with a sensitivity of 92.6 %, specificity of 56.2 %, AUROC curve = 0.766, p= 0.002. The results indicate that FIBROTEST scores above 0.11 correctly report 56.2% of patients without grade 1 LVDD as test negative (true negatives), but 43.8% of patients without grade 1 LVDD are incorrectly identified as test positive (false positives) (Figure 2b). Younger T2DM patients than 56 years old are unlikely to present grade 1 LVDD (AUROC curve = 0.870, sensitivity 76.9 %, specificity 93.7 %, p< 0.001) (Figure 2c). A VAT> 95.9 cm² predicts the LVDD grade 1 with a sensitivity of 40.7 %, specificity of 93.7 %, AUROC curve = 0.674, p= 0.03, indicating that patients with VAT lower than 95.9 cm² will rule out grade 1 LVDD in 93.7% of the cases (Figure 2d).

4. Discussion

T2DM patients are more likely to develop HF, which often occurs following asymptomatic abnormalities. The present study aimed to analyze the interrelation between right and left ventricular function, EAT thickness, and liver function assessed by fibromax in patients with T2DM to identify associated factors with grade 1 LVDD, the first abnormality in the evolution of HF.

We identified in the study group that 80% of the patients presented MASLD, and 54% of the study group had grade 1 LVDD, with no statistical differences in the frequency of grade I LVDD across the different stages of MASLD. The FIBROTEST score was significantly higher in patients with grade I LVDD than those without LVDD. A recent investigation disclosed that 73.3% of cases exhibited grade 1 LVDD upon cardiac ultrasound evaluation. After a year of dapagliflozin treatment, 24.4% of these cases demonstrated remission of grade 1 LVDD. A significant predictor of a favorable therapy response was the decrease in liver fibrosis. Although the study's design did not enable the establishment of causality, it did not delve into the underlying mechanism linking the remission of LVDD and the reduction of liver fibrosis [47,48].

In our study, patients with grade I LVDD demonstrated a significantly higher PCE score of 22.8% compared to 10.8%. Researchers have been exploring distinct phenogroups within HF to enable more targeted interventions: younger, cardiometabolic phenogroup (predominantly female gender with a high prevalence of cardiometabolic and coronary disease), frail phenogroup, which comprises patients with lung disease or atrial fibrillation, respectively inflammatory phenogroup comprised of patients characterized by systemic inflammation with high rates of diabetes and renal dysfunction [49]. The results of this study delineate a portrait of patients with grade 1 LVDD who concurrently present with MASLD, substantial visceral adipose tissue, and surpass the age of 56, accompanied by α2-macroglobulin concentrations higher than 1.92 g/L and liver fibrosis. It is essential to conduct additional research to explore the correlation between these factors and the potential for implementing targeted therapy for α2-macroglobulin to mitigate the likelihood of grade 1 LVDD.

Patients with grade I LVDD were older and had an EAT thickness and VAT higher than those without LVDD. Notably, ɑ2 macroglobulin was significantly higher in patients with grade I LVDD. α2-macroglobulin, a significant plasma glycoprotein primarily produced in the liver, is a large protein synthesized in the body [50], and it is believed that it may be linked to inflammatory responses and properties that promote blood clotting, which could lead to a lack of blood flow. Endothelial dysfunction contributes to the progression of plaque atherosclerosis and the emergence of cardiovascular disease [51]. α2-macroglobulin is a protein with diverse inhibitory functions, including the suppression of endopeptidases. It has been linked to various physiological processes, such as cell growth, inflammation, and coagulation. Research in the Moli-sani cohort study has demonstrated a relationship between α2-macroglobulin and cardiovascular events. Specifically, individuals with high levels of α2-macroglobulin were found to have an increased risk of developing coronary heart disease. Interestingly, this association was observed regardless of the subject's age, gender, or lifestyle [52].

Cardiovascular proteomics has made impressive strides in recent years, with substantial progress in identifying novel biomarkers and elucidating the molecular mechanisms underlying cardiovascular diseases [53].

The data revealed a substantial relationship between α2-macroglobulin and grade I LVDD, a finding that has not been previously documented. However, this research was not without its limitations. First, the study was based on a cross-sectional analysis, which allowed the assessment of only association rather than causal relationships. Second, this single-center study with a small sample size may have resulted in selection bias. Moreover, it is important to account for the potential concomitant effects of medications that target endothelial function, even if they were not administered during the examination.

5. Conclusions

HF is a complicated illness that involves damage to multiple organ systems, such as nonalcoholic MASLD. Although researchers have not yet fully understood the reasons for this link, there are some new and straightforward biomarkers, like α2-macroglobulin or fibrotest, that may indicate both liver stiffness and the likelihood of having grade I LVDD, an early and symptom-free stage of HF in individuals with T2DM.

Supplementary Materials

The following supporting information can be downloaded at the website of this paper posted on Preprints.org.

Author Contributions

Conceptualization, AB and RT; methodology, AB, VI, MMB, LD, BT; software, MMB, VI; validation, AB, BT, and RT; formal analysis, AB and BT; investigation, AB, VI, MMB, and LD; resources, VI and BT; data curation, AB, MMB, and LD; writing—original draft preparation, AB, BT, and RT; writing—review and editing, VI, MMB, and LD; visualization, AB, BT, VI, MMB, LD, and RT; supervision, BT and RT; project administration, AB; funding acquisition, AB, and BT. All authors have read and agreed to the published version of the manuscript.

Funding

We would like to acknowledge the Victor Babes University of Medicine and Pharmacy of Timisoara for covering the publication costs for this research paper and the Association Center for Strategies for Public Health Policies in Diabetes for covering the costs for fibromax tests.

Institutional Review Board Statement

The study was conducted following the principles outlined in the Declaration of Helsinki and received ethical approval from the Ethical Committee at Emergency County Hospital Timisoara (332/October 10, 2022).

Informed Consent Statement

Patients signed written informed consent forms to provide their consent for the study.

Data Availability Statement

Participants in this study did not grant permission for the public release of their data, which is why supporting information is currently unavailable due to the confidential nature of the research.

Acknowledgments

The authors express their gratitude to the researchers from the Diabetes, Nutrition, Metabolic Diseases, and Systemic Rheumatology University Clinic at the "Pius Brinzeu" Emergency Clinical County University Hospital in Timisoara, Romania, for their invaluable help in obtaining the data.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. The frequency distribution of the severity of fibromax test results in studied patients.

Figure 2. Graphical representation of the ROC curve analysis of the factors thresholds diagnostic ability of grade I LVDD in patients with T2DM and MASLD.

Table 1. General characteristics of the studied patients compared by gender.

Variable	Women (n= 27)	Men (n= 23)	p ^a
Age ^a (years)	57.5±11.5	58.6±11.2	0.7
Diabetes duration ^a (years)	8 (21.2)	7 (22.8)	0.6
Abdominal waist ^a (cm)	115 (22.4)	116 (27.1)	0.2
Hip waist ^a (cm)	106 (25.7)	105 (22.1)	0.3
BMI ^a (kg/m²)	35.2 (25.5)	33.3 (25.4)	0.9
VAT ^a (cm²)	55.7 (20.7)	95.7 (31.1)	0.01
FG ^a (mg/dL)	143 (22.4)	158 (29.1)	0.1
HbA1c ^a (%)	7.9 (22.5)	8.1 (25.7)	0.4
Haptoglobin ^a	1.6 (25)	1.6 (25.9)	0.8
ɑ2 Macroglobulin ^a	2 (26.6)	1.9 (24.1)	0.5
Apo A1 ^a	1.5 (29.7)	1.3 (20.5)	0.02
TG ^a (mg/dL)	153 (25)	157 (26)	0.8
TC ^a (mg/dL)	170 (29.2)	135 (21.1)	0.04
HDLc ^a (mg/dL)	46 (28.8)	40.9 (20.2)	0.03
LDLc ^a (mg/dL)	109 (28.6)	85 (19.1)	0.01
Uric Acid ^a (mg/dL)	5.5 (23.5)	5.5 (25.7)	0.5
eGFR ^a (ml/min)	84.1 (16.6)	88.2 (21.1)	0.2
Serum creatinine ^a (mg/dL)	0.7 (21.3)	0.9 (30.3)	0.02
UACr ^a (mg/g)	11.1 (19.8)	6.1 (16.2)	0.3
ALT ^a (u/L)	26 (22.3)	32 (29.2)	0.09
AST ^a (u/L)	20 (24.7)	20 (26.3)	0.7
TB ^a	0.4 (22.4)	0.5 (29)	0.1
FAL ^a	87 (19.5)	76 (15.2)	0.2
GGT ^a	30 (25.4)	32 (25.5)	0.9
EAT ^b (mm)	4±1.5	4.3±1.7	0.6
TAPSE ^a (cm)	2.5 (20)	2.6 (21.1)	0.7
GLS ^a (%)	17.4 (11.5)	14.2 (5.4)	0.02
LVEF ^a (%)	56 (21.1)	58 (24.2)	0.4
LA ^a (cm)	3.6 (19.7)	3.8 (25.1)	0.1
MV s` lateral wall ^a (cm/s)	8 (19.7)	9 (23.8)	0.2
MV s` septum ^a (cm/s)	7 (20.9)	7.5 (23.5)	0.4
DTE ^a (ms)	204 (21)	207 (21)	1
MV a' lateral wall ^a (cm/s)	10.5 (19.3)	12 (23.2)	0.2
MV a` septum ^a (cm/s)	10 (18.1)	11 (26.5)	0.02
Ascending AO ^b (cm)	2.7±0.4	3.0±0.2	0.04
MV A-wave Vmax ^a (cm/s)	82 (25.1)	76.5 (17.6)	0.05
MV E-wave Vmax ^a (cm/s)	67 (22.9)	63 (21.9)	0.8
average E/e` ^a	7 (20.6)	7.1 (21.5)	0.8
MV e` septum ^a (cm/s)	8 (23.1)	8.5 (20.4)	0.4
MV e` lateral wall ^a (cm/s)	10 (19.7)	10.5 (23.8)	0.2
PCE risk ^a (%)	15.2 (13)	27.9 (17.8)	0.1
FIBROTEST score ^a	0.1 (21.6)	0.2 (30)	0.04
ACTITEST score ^a	0.1 (21.3)	0.1 (30.3)	0.03
ASHTEST score ^a	0.01 (29.2)	0.0 (21)	0.03
NASHTEST score ^a	0.5 (26.4)	0.5 (23.3)	0.4
STEATOTEST score ^b	0.6±0.1	0.6±0.1	0.9

Abbreviations: BMI = body mass index; VAT= visceral adipose tissue; FG= fasting glycemia; HbA1c= glycated hemoglobin A1c; Apo A1= Apolipoprotein AI; TG= triglycerides; TC= total cholesterol; HDLc= high-density lipoprotein cholesterol; LDLc= low-density lipoprotein cholesterol; eGFR= estimated glomerular filtration rate; UACr= urinary albumin/creatinine ratio; ALT= alanine aminotransferase; AST= aspartate aminotransferase; TB= total bilirubin; FAL= phosphatase alkaline; GGT= gamma-glutamyl transferase; EAT= epicardiac adipose tissue; TAPSE= tricuspid annular plane systolic excursion; GLS= global longitudinal strain; LVEF= left ventricle ejection fraction; LA= Left atrium; MV= mitral valve; DTE= E-wave de; AO= aorta; PCE risk= Pooled cohort risk predictor of the 10-year risk for a first ASCVD event. ^a Mann-Whitney test, ^b T-test.

Table 2. Comparison of studied factors by grade I LVDD presence in patients with T2DM.

Variable	With LVDD grade I (n= 27)	Without LVDD grade I (n= 23)	∆	p ^a
Age ^a (years)	63.5 (27.4)	50.5 (11.8)	-13.1	0.0001
Diabetes duration ^a (years)	9 (19.8)	7 (15.7)	-3.5	0.2
Abdominal waist ^a (cm)	115 (22.9)	112.5 (19.1)	-3	0.3
Hip waist ^a (cm)	104 (20.7)	104 (20.1)	-2	0.8
BMI ^a (kg/m²)	34.2 (22.7)	34.2 (20.7)	-1.5	0.6
VAT ^b (cm²)	86.2±46.9	57.7±33.9	-28.4	0.04
FG ^b (mg/dL)	145.4± 36.6	143± 36.3	-2.4	0.8
HbA1c ^a (%)	7.7 (17.8)	8.8 (24.9)	0.8	0.06
Haptoglobin ^b (g/L)	1.8± 0.6	1.5± 0.3	-0.2	0.08
ɑ2 Macroglobulin ^a (g/L)	2.2 (26.5)	1.6 (14.3)	-0.5	0.002
Apo A1 ^a (g/L)	1.4 (22.8)	1.4 (20.5)	-0.0	0.5
TG ^a (mg/dL)	117 (20.1)	167.5 (25.1)	8.3	0.2
TC ^b (mg/dL)	158.2±47.4	168.6±46.1	10.4	0.4
HDLc ^a (mg/dL)	42 (22.3)	42.5 (21.4)	-0.9	0.8
LDLc ^a (mg/dL)	91 (18.9)	111.5 (24.2)	15.2	0.1
Uric Acid ^a (mg/dL)	5.6 (24)	5.3 (18.4)	-0.3	0.1
eGFR ^b (ml/min)	82.7±18.3	95±16.6	12.2	0.06
Serum creatinine ^b (mg/dL)	0.8±0.1	0.7±0.1	-0.0	0.6
UACr ^a (mg/g)	13 (17)	5.7 (11.1)	-58.3	0.07
ALT ^a (u/L)	27 (19.6)	30.5 (25.9)	-3.9	0.1
AST ^b (u/L)	22.8±16.7	19.3±4.4	-3.5	0.4
TB ^b (µmol/L)	0.6±0.4	0.6±0.3	-0.0	0.9
FAL ^b	83.9±22.8	81.9±33	-2	0.8
GGT ^a (u/L)	29 (21.9)	31 (22.1)	-13.3	0.9
EAT ^b (mm)	4.6±1.5	3.4±1.6	-1.2	0.01
TAPSE ^a (cm)	2.5 (17.5)	2.8 (23.5)	0.2	0.1
GLS ^a (%)	15.5 (8.8)	17 (10.1)	0.7	0.6
LVEF ^a (%)	56 (20.6)	56.5 (24.3)	2.0	0.3
LA ^a (cm)	3.9 (24.9)	3.3 (14.9)	-0.4	0.009
MV s` lateral wall ^a (cm/s)	8 (16.6)	10 (27.8)	2.2	0.002
MV s` septum ^a (cm/s)	7 (20.4)	7 (23.2)	0.3	0.4
DTE ^a (ms)	219.5 (27.6)	183 (9.4)	-56.9	<0.0001
MV a' lateral wall ^a (cm/s)	12 (20.5)	12 (21.6)	0.4	0.7
MV a` septum ^a (cm/s)	10.5 (22.5)	9.5 (19.7)	-0.3	0.4
Ascending AO ^b (cm)	2.9 ± 0.3	2.6±0.3	-0.3	0.005
MV A-wave Vmax ^a (cm/s)	86 (28)	66 (11.8)	-20.1	<0.0001
MV E-wave Vmax ^a (cm/s)	60 (18)	71.5 (28.7)	11.8	<0.0001
average E/e` ^a	7.6 (24.4)	6.7 (15.6)	-1.4	<0.0001
MV e` septum ^a (cm/s)	7 (15.6)	10.5 (30.9)	3.5	0.0001
MV e` lateral wall ^a (cm/s)	8 (15.1)	12 (30.1)	3.7	0.0001
PCE risk ^a (%)	22.8 (15.1)	10.8 (7.5)	-13.7	0.02
FIBROTEST score ^a	0.19 (26.2)	0.11 (14.8)	-0.1	0.003
ACTITEST score ^a	0.1 (21)	0.1 (23.5)	-0.01	0.5
ASHTEST score ^a	0.01 (24.1)	0 (18.4)	-0.005	0.1
NASHTEST score ^a	0.5 (22)	0.5 (20.5)	-0.01	0.6
STEATOTEST score ^b	1.9±0.8	2.1±0.6	0.2	0.3

Abbreviations: ∆= mean difference between group variable; BMI = body mass index; VAT= visceral adipose tissue; FG= fasting glycemia; HbA1c= glycated hemoglobin A1c; Apo A1= Apolipoprotein AI; TG= triglycerides; TC= total cholesterol; HDLc= high-density lipoprotein cholesterol; LDLc= low-density lipoprotein cholesterol; eGFR= estimated glomerular filtration rate; UACr= urinary albumin/creatinine ratio; ALT= alanine aminotransferase; AST= aspartate aminotransferase; TB= total bilirubin; FAL= phosphatase alkaline; GGT= gamma-glutamyl transferase; EAT= epicardiac adipose tissue; TAPSE= tricuspid annular plane systolic excursion; GLS= global longitudinal strain; LVEF= left ventricle ejection fraction; LA= Left atrium; MV= mitral valve; DTE= E-wave deceleration time; AO= aorta; PCE risk= Pooled cohort risk predictor of the 10-year risk for a first ASCVD event. ^a Mann-Whitney test ^b T-test.

Table 3. Regression analysis of grade I LVDD and studied factors in patients with T2DM.

Parameter	R²	Coefficient	Standard Error	95% CI	t	p
Age (years)	0.39	0.03	0.00	0.01 - 0.04	5.1	<0.0001
Diabetes duration (years)	0.06	0.01	0.01	-0.00 - 0.04	1.5	0.1
Abdominal waist (cm)	0.02	0.00	0.00	-0.00 - 0.02	0.9	0.3
Hip waist (cm)	0.00	0.00	0.00	-0.00 - 0.01	0.5	0.6
BMI (kg/m²)	0.01	0.00	0.01	-0.01 - 0.03	0.7	0.4
VAT (cm²)	0.09	0.00	0.00	0.00 - 0.00	2.1	0.04
TyG index	0.00	-0.16	0.26	-0.69 - 0.36	-0.6	0.5
FG (mg/dL)	0.01	-0.00	0.00	-0.00 - 0.00	-0.8	0.4
HbA1c (%)	0.05	-0.06	0.04	-0.16 - 0.02	-1.5	0.1
Haptoglobin (g/L)	0.06	0.23	0.13	-0.03 - 0.51	1.7	0.08
ɑ2 Macroglobulin (g/L)	0.16	0.33	0.11	0.09 - 0.56	2.8	0.006
Apo A1 (g/L)	0.00	0.06	0.27	-0.48 - 0.62	0.2	0.8
TG (mg/dL)	0.02	0.00	0.00	-0.00 - 0.00	1.0	0.2
TC (mg/dL)	0.00	-0.00	0.00	-0.00 - 0.00	-0.5	0.5
HDLc (mg/dL)	0.00	0.00	0.00	-0.01 - 0.01	0.2	0.8
LDLc (mg/dL)	0.03	-0.00	0.00	-0.00 - 0.00	-1.2	0.2
Uric Acid (mg/dL)	0.02	0.06	0.06	-0.06 - 0.18	0.9	0.3
eGFR (ml/min)	0.11	-0.00	0.00	-0.01 - 0.00	-1.9	0.06
Serum creatinine (mg/dL)	0.00	0.20	0.41	-0.63 - 1.04	0.5	0.6
UACr (mg/g)	0.05	0.00	0.00	-0.00 - 0.00	1.2	0.2
ALT (u/L)	0.00	-0.00	0.00	-0.01 - 0.00	-0.3	0.6
AST (u/L)	0.01	0.00	0.00	-0.00 - 0.01	0.8	0.4
TB (µmol/L)	0.00	0.02	0.19	-0.37 - 0.41	0.1	0.9
FAL	0.00	0.00	0.00	-0.00 - 0.00	0.1	0.8
GGT (u/L)	0.02	0.00	0.00	-0.00 - 0.00	0.9	0.3
EAT (mm)	0.13	0.10	0.04	0.02 - 0.19	2.5	0.01
TAPSE (cm)	0.16	0.37	0.13	0.10 - 0.64	2.7	0.008
GLS (%)	0.02	-0.03	0.05	-0.13 - 0.07	-0.6	0.5
LVEF (%)	0.02	-0.01	0.01	-0.03 - 0.01	-1.0	0.3
LA (cm)	0.16	0.37	0.13	0.10 - 0.64	2.7	0.008
MV s` lateral wall (cm/s)	0.24	-0.10	0.02	-0.16 - -0.04	-3.5	0.001
MV s` septum (cm/s)	0.01	-0.03	0.04	-0.12 - 0.06	-0.6	0.5
DTE (ms)	0.40	0.00	0.00	0.00 - 0.00	5.1	<0.0001
MV a` lateral wall (cm/s)	0.00	-0.01	0.02	-0.07 - 0.04	-0.5	0.6
MV a` septum (cm/s)	0.00	0.01	0.03	-0.04 - 0.07	0.4	0.6
Ascending AO (cm)	0.17	0.50	0.17	0.15 - 0.85	2.9	0.005
MV A-wave Vmax (cm/s)	0.27	0.01	0.00	0.00 - 0.02	3.9	0.0003
MV E-wave Vmax (cm/s)	0.17	-0.01	0.00	-0.02 - -0.00	-2.9	0.005
average E/e`	0.39	-0.12	0.02	-0.17 - -0.07	-4.9	<0.0001
MV e` septum (cm/s)	0.38	-0.10	0.02	-0.15 - -0.06	-5.0	<0.0001
MV e` lateral wall (cm/s)	0.37	-0.10	0.02	-0.14 - -0.05	-4.8	<0.0001
PCE risk (%)	0.18	0.01	0.00	0.00 - 0.02	2.2	0.03
FIBROTEST score	0.10	0.94	0.42	0.09 - 1.80	2.2	0.03
ACTITEST score	0.00	0.21	0.66	-1.12 - 1.55	0.3	0.7
ASHTEST score	0.07	12.25	6.86	-1.61- 26.13	1.7	0.08
NASHTEST score	0.00	0.10	0.44	-0.78 - 0.99	0.2	0.8
STEATOTEST score	0.01	-0.40	0.49	-1.41 - 0.60	-0.8	0.4

Abbreviations: LVDD= left ventricle diastolic dysfunction; coefficient of determination R²; BMI = body mass index; VAT= visceral adipose tissue; FG= fasting glycemia; HbA1c= glycated hemoglobin A1c; Apo A1= Apolipoprotein AI; TG= triglycerides; TC= total cholesterol; HDLc= high-density lipoprotein cholesterol; LDLc= low-density lipoprotein cholesterol; eGFR= estimated glomerular filtration rate; UACr= urinary albumin/creatinine ratio; ALT= alanine aminotransferase; AST= aspartate aminotransferase; TB= total bilirubin; FAL= phosphatase alkaline; GGT= gamma-glutamyl transferase; EAT= epicardiac adipose tissue; TAPSE= tricuspid annular plane systolic excursion; GLS= global longitudinal strain; LVEF= left ventricle ejection fraction; LA= Left atrium; MV= mitral valve; DTE= E-wave deceleration time; AO= aorta; PCE risk= Pooled cohort risk predictor of the 10-year risk for a first atherosclerotic cardiovascular disease (ASCVD) event.

Table 4. Logistic regression analysis of risk factors of grade I LVDD.

Variable	Exp (β)	Nagelkerke R²	Standard Error	Wald	p
Age (years)	1.25	0.54	3.77	10.39	0.001
VAT (cm²)	1.01	0.13	0.00	3.85	0.04
ɑ2 Macroglobulin (g/L)	6.05	0.23	1.42	4.49	0.03
EAT (mm)	1.94	0.20	0.31	4.50	0.03
TAPSE (cm)*	0.89	0.00	0.21	0.25	0.6
LA (cm)	6.43	0.22	0.77	5.82	0.01
MV s` lateral wall (cm/s)	0.56	0.31	0.21	7.31	0.006
DTE (ms)	1.17	0.75	0.06	6.86	0.008
Ascending AO (cm)	12.01	0.23	0.98	6.42	0.01
MV A-wave (cm/s)	1.08	0.37	0.02	8.02	0.004
MV E-wave (cm/s)	0.93	0.22	0.02	6.39	0.01
average E/e`	1.67	0.19	0.24	4.58	0.03
MV e` septum (cm/s)	0.46	0.51	0.25	9.26	0.002
MV e` lateral wall (cm/s)	0.53	0.49	0.19	10.49	0.001
PCE risk (%)*	1.12	0.32	0.06	3.77	0.05
FIBROTEST score	309.2	0.16	2.88	3.94	0.04

Abbreviations: LVDD= left ventricle diastolic dysfunction; coefficient of determination R²; VAT= visceral adipose tissue; EAT= epicardiac adipose tissue; TAPSE= tricuspid annular plane systolic excursion; LA= Left atrium; MV= mitral valve; DTE= E-wave deceleration time; AO= aorta; PCE risk= Pooled cohort risk predictor of the 10-year risk for a first atherosclerotic cardiovascular disease (ASCVD) event.

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