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Submitted:
04 June 2024
Posted:
05 June 2024
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Kruskal-Wallis | Dunn/Bonferroni p-value | |||
Gene | p-value | TN vs HS | TN vs HSAX | HS vs HSAX |
NFKB1 | 0.0112 | 0.0283 | ns | 0.0283 |
RELA | 0.0119 | 0.0088 | ns | ns |
IKBKB | 0.0090 | 0.0105 | 0.0694 | ns |
NFE2L2 | 0.0394 | 0.0331 | ns | ns |
KEAP1 | 0.0112 | 0.0283 | 0.0283 | ns |
MAF | 0.0298 | 0.0241 | ns | ns |
PPARA | 0.0644 | 0.0601 | ns | ns |
RXRA | 0.0041 | 0.0029 | ns | ns |
PPARG | 0.0034 | 0.0105 | 0.0105 | ns |
CD36 | 0.0417 | 0.0448 | ns | ns |
SOD1 | 0.1284 | ns | ns | ns |
SOD2 | 0.0489 | ns | ns | 0.0694 |
SOD3 | 0.0446 | 0.0386 | ns | ns |
CAT | 0.2911 | ns | ns | ns |
GPX1 | 0.2086 | ns | ns | ns |
GPX2 | 0.0050 | 0.0061 | ns | 0.0448 |
GPX3 | 0.0852 | ns | ns | ns |
GPX4 | 0.2359 | ns | ns | ns |
PRDX1 | 0.1169 | ns | ns | ns |
PRDX3 | 0.1345 | ns | ns | ns |
PRDX4 | 0.0260 | 0.0283 | ns | ns |
PRDX6 | 0.0060 | 0.0061 | ns | 0.0694 |
BCL2 | 0.3123 | ns | ns | ns |
CASP3 | 0.0005 | ns | ns | 0.0002 |
TP53 | 0.5671 | ns | ns | ns |
Ingredients (%) | Starter | Finisher |
---|---|---|
Corn | 54.86 | 63.14 |
Soybean Meal | 39.99 | 30.09 |
Soybean oil | 2.00 | 4.50 |
Limestone | 1.27 | 0.85 |
Monocalcium phosphate | 0.75 | 0.50 |
Lysine (%) | 0.23 | 0.18 |
Methionine (%) | 0.14 | 0.12 |
Threonine (%) | 0.20 | 0.16 |
Sodium chloride (%) | 0.43 | 0.35 |
Sodium bicarbonate | 0.12 | 0.10 |
Vitamin-mineral premix | 0.50 | 0.50 |
Astaxanthin supplement¹ | 0.01 | 0.01 |
Total | ||
Calculated analysis | ||
ME (kcal/kg) | 2909 | 3203 |
CP (%) | 22.09 | 18.07 |
Calcium (%) | 0.75 | 0.52 |
Total Phosphorus (%) | 0.57 | 0.47 |
digPhosphorous (%) | 0.30 | 0.23 |
Lysine (%) | 1.39 | 1.10 |
dig Lysine (%) | 1.25 | 0.99 |
Methionine (%) | 0.48 | 0.41 |
dig Methionine (%) | 0.45 | 0.39 |
Cysteine (%) | 0.43 | 0.38 |
Threonine (%) | 1.03 | 0.85 |
dig Threonine (%) | 0.85 | 0.69 |
Tryptophan (%) | 0.33 | 0.26 |
Methionine + cysteine (%) | 0.91 | 0.80 |
Arginine (%) | 1.61 | 1.31 |
Valine (%) | 1.22 | 1.03 |
Isoleucine (%) | 0.93 | 0.76 |
Leucine (%) | 1.89 | 1.63 |
Neutral detergent fiber | 9.13 | 8.78 |
Crude fiber | 3.97 | 3.46 |
Sodium | 0.22 | 0.18 |
Chloride | 0.30 | 0.25 |
Choline (mg/kg) | 1419 | 1200 |
Astaxanthin (mg/kg) | --- | 1.33 |
Gene | NCBI Accession No. | Primer set (5’-3’) |
β-ACTIN | NM_205518.2 | F:5' - AATTGTGCGTGACATCAAGG |
TBP GAPDH |
NM_205103.2 NM_204305.2 |
R:3' -CACAGGACTCCATACCCAAG F:5' -GCGGCAGGCTCTGTT R:3' -ACCGAAAAGGTTTTTGACCC F:5' -AAGTCGGAGTCAACGGATTT R:3' -TCACAAGTTTCCCGTTCTCA |
NFKB1 RELA IKBKB |
NM_205134.2 NM_001396038.1 NM_001395965.1 |
F:5’ -GGACGGCGAAAGGACTCT R:3’ -CCATTGCAAACATTTGGGGAT F:5’ -CGGTTCCGCTATAAGTGTGA R:3’ -GTAATGGTTTACGCGGATGG F:5’ -TCCCTGGGAGATGAAGGAG R:3’ -TTTGGATGGTTCAGCCTCTT |
NFE2L2 KEAP1 MAF PPARA RXRA PPARG CD36 |
XM_015287264.4 GenBank: KU321503.1 NM_001044671.1 NM_001001464.1 XM_003642291.6 NM_001001460.2 NM_001030731.1 |
F:5’ –CAGGGGTAGCAAGGTATGAG R:3’ -TGCCTCCAAAGGATGTCAAT F:5’ –GATCGACGGGATGATCTACG R:3’ –GGCGTACAGCAGTATGTTCA F:5’ –CCAGAGTTTTTCATGTACCCG R:3’ -CTTTGTAGCTGTCTTCGTGC F:5’ – AGCCACTTGCTATCACCAAT R:3’ – ACTTAAACTCCTTTATGATTCTGGT F:5’ –CTTCCTGCCACTGGATTTCT R:3’ –CTGATGACGGAGAAGGGTG F:5’ – CTTGACAGCGCCAGAGATTA R:3’ –GATTGCACTTTGGCAATCCT F:5’ –TTTCTTGCAAAGCAGGAGGTT R:3’ –CTGATCTTCGTGAGAGAAGCTGTA |
SOD1 | NM_205064.2 | F:5' -AAAAGATGCAGATAGGCACG |
R:3' -TTATCTCCCCCTCTACCCAG | ||
SOD2 SOD3 |
NM_204211.2 XM_040699307.2 |
F:5' -CCTTCGCAAACTTCAAGGAG R:3' -AGCAATGGAATGAGACCTGT F:5’ –CAACTCGCAAACAACGCT R:3’ –CTGGTGAGTGAGAACCTGC |
CAT | NM_001031215.2 | F:5' -TTCCACGTTAAGACCGATCA |
R:3' -CAATCTTGCCCACTGGAATG | ||
GPX1 | NM_001277853.3 | F:5' -AATTCGGGCACCAGGAGAA |
R:3' - CTCGAACATGGTGAAGTTGG | ||
GPX2 | NM_001277854.3 | F:5' -AGTTCGGCTACCAGGAGAA |
R:3' -CTTCTGGAACAGGGTGAAGT | ||
GPX3 | NM_001163232.3 | F:5' - AGGTGAAATGCTACGACTCC |
GPX4 PRDX1 PRDX3 PRDX4 PRDX6 BCL2 CASP3 TP53 |
NM_204220.3 NM_001271932.2 XM_426543.6 XM_046912353.1 NM_001039329.3 NM_205339.3 NM_204725.1 NM_205264.1 |
R:3' - AGTGCATTCAGTTCGAGGTA F:5’ –AATGTGCGCTCAGGCG R:3’ –AGACGAAGCCCCTGTACT F:5’ –TGCGGGGCTCTTTGTATTAAA R:3’ –ATTGCCCATCTGGCATTACA F:5’ –CGTTGTCAATGGGGAGTTC R:3’ –GGGGCACACAAAGGTGAAAT F:5’ –ATCCCCTTGACTTCACGTTT R:3’ –ATCTTCATTGGTCCGAGTCC F:5’ –GACATCAACGCCTACAATGG R:3’ –GGCCAAATATGAACACCACA F:5’ –GAGGATGGGATGCCTTTGTG R:3’ –CCACGATAAACTGGGTGACT F:5’ – GGTGGAGGTGGAGGAGC R:3’ –TGAGCGTGGTCCATCTTTTA F:5’ –GTTACCACGACGAGCCACCAA R:3’ –TGCAGCGCCTCATTGATCTCCTT |
Kruskal-Wallis |
Dunn/Bonferroni p-value |
||||||
Measurements | TN | HS | HSAX | p-value | TN vs HS | TN vs HSAX | HS vs HSAX |
BW (g) | 2673.68 ± 35.71 | 1848.85 ± 61.56 | 1867.83 ± 60.82 | 0.0047 | 0.0095 | 0.0278 | ns |
ADFI (g) | 94.98 ± 1.45 |
70.68 ± 72.24 |
72.24 ± 1.14 |
0.0038 | 0.0047 | 0.0500 | ns |
ADGR (g) | 62.65 ± 0.86 |
42.99 ± 1.46 |
43.45 ± 1.44 |
0.0047 | 0.0095 | 0.0278 | ns |
FCR | 1.52 ± 0.02 |
1.64 ± 0.02 |
1.67 ± 0.03 |
0.0049 | 0.0226 | 0.0119 | ns |
Gene | Cellular component | Molecular function | Biological process | Transcript IDs | ||||
---|---|---|---|---|---|---|---|---|
NFKB1 (nuclear factor kappa B subunit 1) | nucleus, cytoplasm, mitochondrion, chromatin | DNA binding transcription factor activity, RNA polymerase II specific DNA binding, chromatin, protein and actinin binding | regulation of transcription by RNA polymerase II, MAPK cascade, JNK cascade, NIK/NF-kappaB signaling | ENSGAL00010005476 | ||||
RELA (proto-oncogene, NF-kB subunit) | cytoplasm | DNA-binding transcription factor activity | regulation of DNA-templated transcription | ENSGALG00010001277 | ||||
IKBKB (inhibitor of nuclear factor kappa B kinase subunit beta) | cytoplasm, cytosol | protein kinase activity, identical protein binding, protein homodimerization activity, scaffold protein binding, transferrin receptor binding | Protein phosphorylation, I-kappaB kinase/NF-kappaB signaling, cellular response to tumor necrosis factor, regulation of establishment of endothelial barrier, negative regulation of bicellular tight junction assembly | ENSGALG00010017955 | ||||
NFE2L2 (nuclear factor, erythroid 2 like 2) | nucleus, cytoplasm, Golgi apparatus, chromatin, centrosome, plasma membrane, RNA polymerase II transcription regulator complex | DNA binding transcription factor activity, RNA polymerase II specific DNA-binding transcription factor binding, ubiquitin protein ligase binding | response to oxidative stress, inflammatory response, regulation of gene expression, protein ubiquitination, cell redox homeostasis, positive regulation of glutathione biosynthetic process, regulation of removal of superoxide radicals | ENSGAL00010024107 | ||||
KEAP1 (kelch like ECH associated protein 1) | nucleus, cytoplasm, endoplasmic reticulum, Cul3-RING ubiquitin ligase complex, centriolar satellite | RNA polymerase II-specific DNA-binding transcription factor binding, ubiquitin ligase-substrate adaptor activity, disordered domain specific binding, identical protein binding | cellular response to oxidative stress, ubiquitin-dependent protein catabolic process, regulation of DNA-templated transcription, regulation of autophagy | ENSG00000079999 | ||||
MAF (MAF bZIP transcription factor) | nucleus, cytoplasm, RNA polymerase II transcription regulator complex | DNA-binding transcription factor activity, RNA polymerase II sequence-specific DNA binding | regulation of DNA templated transcription, regulation of transcription by RNA polymerase II, cell development | ENSGALG00010007800 | ||||
RXRA (retinoid X receptor alpha) | nucleus, RNA polymerase II transcription regulator complex, receptor complex | RNA polymerase II transcription regulatory region sequence-specific DNA binding, enzyme, peptide, metal ion binding, retinoic acid-responsive element binding, Vitamin D response element binding | peroxisome proliferator activated receptor signaling pathway, retinoic acid receptor signaling pathway, positive regulation of Vitamin D receptor signaling pathway, cell differentiation | ENSGALG00010028422 | ||||
PPARG (peroxisome proliferator-activated receptor gamma) | nucleus, cytoplasm, intracellular membrane-bounded organelle | DNA-binding transcription factor activity, nuclear receptor activity, zinc and metal ion binding | Regulation of DNA-templated transcription, transcription by RNA polymerase II, intracellular receptor signaling pathway | ENSGALG00010027917 | ||||
CD36 (CD36 molecule) | external side of plasma membrane, receptor complex, membrane raft | low-density lipoprotein particle receptor activity, high-density lipoprotein particle binding, Toll-like receptor binding, scavenger receptor activity | MAPK cascade, cell surface receptor signaling pathway, positive regulation of cytosolic calcium ion concentration, nitric oxide mediated signal transduction, intestinal cholesterol absorption, positive regulation of reactive oxygen species metabolic process, lipid transport across blood-brain barrier | ENSGALG000010008392 | ||||
SOD2 (superoxide dismutase 2, mitochondrial) | mitochondrion | superoxide dismutase activity, oxidoreductase activity, manganese ion binding, metal ion binding, identical protein binding | response to oxidative stress, oxidation-reduction process, negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway, response to hydrogen peroxide, removal of superoxide radicals | ENSGALT00000019062 | ||||
SOD3 (superoxide dismutase 3) | extracellular space, collagen- containing extracellular matrix | superoxide dismutase activity, copper and metal ion binding | superoxide metabolic process, response to hypoxia | ENSGALG00010009833 | ||||
GPX2 (glutathione peroxidase 2) | cytosol, intercellular bridge, mitotic spindle | glutathione peroxidase activity, phospholipid-hydroperoxide glutathione peroxidase activity | response to oxidative stress | ENSGALG00010021537 | ||||
PRDX4 (peroxredoxin 4) | cytoplasm, endoplasmic reticulum | antioxidant activity, thioredoxin peroxidase activity, oxidoreductase activity, peroxiredoxin activity, molecular sequestering activity | response to oxidative stress, cell redox homeostasis, hydrogen peroxide catabolic process, reactive oxygen species metabolic process, cellular oxidant detoxification | ENSGALG00010003214 | ||||
PRDX6 (peroxredoxin 6) | nucleus, cytoplasm | antioxidant activity, glutathione peroxidase activity, oxidoreductase activity, peroxiredoxin activity, ubiquitin protein ligase binding | response to oxidative stress, cellular oxidant detoxification, glycerophospholipid catabolic process | ENSGALG00010013528 | ||||
CASP3 (caspase 3) | nucleus, cytoplasm | endopeptidase activity, hydrolase activity, cyclin-dependent protein serine/threonine kinase inhibitor activity, cysteine-type endopeptidase activity involved in execution phase of apoptosis | T and B cell homeostasis, negative regulation of cytokine production, intrinsic apoptotic signaling pathway, negative regulation of cell cycle, neuron apoptotic process, epithelial cell apoptotic process | ENSGALG00010007067 |
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