Submitted:
03 June 2024
Posted:
05 June 2024
Read the latest preprint version here
Abstract
Background:
Worldwide, Swine and Avian influenza, or the H1N1 and H5N1 serotypes of the Influenza A virus, is a highly contagious illness. In recent years, the global incidence of mortality rate has surpassed 284,000 fatalities.
Aim of the study:
The production of mRNA vaccine lipid nanoparticles (LNP) to combat the deadly strains of Avian and Swine influenza (H1N1 and H5N1 of the influenza A serotype).
Methodology:
Polymerase chain reaction (PCR) was used to clone genes of interest encoding Haemagglutinin (HA) and Neuraminidase (NA) of Influenza A virus strains (H5N1 and H1N1) known as Avian flu and Swine flu, respectively. The genes were then inusing the EcoR1, BAM Hind II restriction endonuclease type II, and Ligase enzyme into the pET-21(+) transcription vector plasmid. On the other hand, the hybrid transcription vectors were mixed with T7 RNA polymerase and ribonucleotides within a test tube after being linearized using EcoR II. Following their purification via reversed-phase high-performance liquid chromatography (RP-HPLC), the mRNAs of the HA and NA were encased in 70 nm LNPs and subjected to reverse-phase high-performance liquid chromatography (RP-HPLC). Lastly, immunogenicity studies were performed in stages 1/2 of randomized human and preclinical clinical trials.
Results:
The bivalent vaccination had around 63% efficacy in preclinical studies and approximately 60% in randomized human clinical trials. The ELISA test showed strong neutralizing antibodies against HA were prevalent.
Conclusion:
The current study is a promising effort because of the development of the novel LNP-mRNA vaccine, which could lower the incidence of Avian and Swine flu infectious disorders.
Keywords:
nfection
; mRNA
; Vaccine
; Swine
; Avian
; Influenza
; Nanoparticles
; Lipid
INTRODUCTION
Influenza A virus-related Swine (H1N1) and Avian (H5N1) flu infections are dangerous, widely prevalent infectious illnesses.[1] An influenza outbreak in humans was brought on by a new strain of Influenza A (H1N1) originating in pigs in April 2009.[2] It originated in Mexico, moved to the USA, and eventually to 208 other nations.[3] There were millions of instances worldwide as of December 2009.[4] There were 9596 fatalities worldwide, including 1445 of them in the United States.[5] The World Health Organization (WHO) classified the Swine flu as a category 6 pandemic in June 2009, making it abundantly evident that action and mitigation measures were required.[6] Rarely do pandemics brought on by the H1N1 or H5N1 Influenza virus arise, although significant outbreaks in the winter months happen almost yearly.[7]
A pandemic happens when an Influenza A virus variation carries a novel haemagglutinin to which no human neutralizing antibodies have been found.[8] There are only Influenza virus in the Orthomyxovirus family.[9] The Influenza virus has a segmented RNA genome, which makes mutations easier to develop.[10] Influenza viruses are tiny in size.[11] On several spikes, HA and NA are found.[12] The annual number of deaths in the United States from influenza is estimated to be close to 36,000.[13] The medication included Zanamivir and Oseltamivir, which were successfully used to combat strains A, H5N1, and H1N1 during the worldwide pandemic flu outbreak.[14] The Influenza A vaccination (H1N1 and H5N1) was shown to be the primary means of prevention.[15] Worldwide, there are two primary varieties of flu vaccinations that are accessible: trivalent and quadrivalent vaccines.[16] It was shown that humoral immunity, which produces neutralizing antibodies against HA, is the primary defense against many flu viruses.[17] The current work set out to create a new bivalent LNP-mRNA flu vaccine that would target the HA and NA of Influenza A (H1N1 and H5N1), two strains of the virus that cause deadly flu illnesses every winter throughout the world.
METHODOLOGY
Ethical Statement:
All pertinent national, institutional, and/or worldwide guidelines for the care and use of humans and animals were postdated in the current study. The ethical committee for human and animal handling at Cairo University (ECAHCU), the pharmacy faculty at the University of Cairo, Egypt, and the local authorities approved all procedures used in the study, including those involving humans and animals, in accordance with the Weatherall report's recommendations (approval number PXV359/2023). Every attempt was made to reduce the number of people and animals used in the study, as well as their suffering. Randomized human clinical trial registration number CTX628/2023 was obtained and authorized by EDA.
Source of Animal Models:
A hundred male mice weighing between 40 and 50 grams were procured and approved for legalization from the department of pharmacology and toxicology at Cairo University's college of pharmacy in Egypt.
Collection of the Samples:
A hundred blood samples were collected from Egyptian patients suffering from Influenza A (H1N1 and H5N1) viruses.
Inclusion Criteria for Animal Models:
Adult male mice weighing 40-50 gm which could be infected by Influenza A ( H1N1,H5N1) viruses after inoculation with an infectious dose of 0.6-3.0 TCID50 for each strain through an intranasal route of administration for exploding expression of viral proteins and exciting potent humoral immunity. Incubation time ranged from 3-5days for the appearance of symptoms to fall out.
Exclusion Criteria:
Young mice and Pregnant female mice.
Material:
All chemical and biological components were acquired from Alnasr Pharmaceutical Company, Abo zabal Alkhanka, Qalyobia, Egypt, and Algomhoria Pharmaceutical Company, Cairo, Egypt.
Place and Date of the Study:
Between January 2023 and May 2024, this study was conducted at Cairo University's pharmacy faculty in Egypt.
Type of Study:
Screening experimental study.
Methods:
The principle of manufacture of mRNA vaccine included polymerase chain reaction (PCR) used to clone genes of interest encoding Haemagglutinin (HA) and Neuraminidase (NA) of Influenza A virus strains (H5N1 and H1N1) known as Avian flu and Swine flu, respectively. The genes were then inserted using the EcoR1, BAM Hind II restriction endonuclease type II, and Ligase enzyme into the pET-21(+) transcription vector plasmid. On the other hand, the hybrid transcription vectors were mixed with T7 RNA polymerase and ribonucleotides within a test tube after being linearized using EcoR II. Open reading frames of genes encoding the spike proteins HA and NA of A (H1N1,H5N1) influenza were constructed and identified using nucleotide sequence deposited on NCBI website and BLASTn software. TACATCCAGCCACAATTGGA was the forward primer utilized for the polymerase chain reaction (PCR) cloning of cDNA of HA; while the reverse primer was TGTGTGCTCTTTTGGTCAGC. On the other hand, the forward primer for PCR cloning of NA was AGGGAGCCTTGCTGAATGAC; whereas the reverse primer used was ATCATTGGGGCGTGGATTGT. The polymerase chain (PCR) was exploited in the cloning process using PCR SuperMix kit with Catalog number 10572014 (purchased from Invitrogen, USA). PCR steps included denaturation, annealing and Extension. The four components (reagents and chemicals) were mixed in a centrifuge tube; then inserted into the PCR apparatus. The procedure was run according to the PCR kit manufacturer's instructions. The reaction mixture comprised a segment of DNA sample, DNA primers, DNA polymerase and nucleotide solution mixture containing adenine, thymine, cytosine and guanine (A,T,C,G). During the denaturation cycle, the solution enclosing reaction mixture was heated up to 94 ℃ using a thermal cycler; furthermore the sample mixture was cooled to 50 ℃ allowing annealing cycle to occur. Finally, the extension cycle comprising the extension of DNA occurred when the temperature cycled from 95 ℃ to 50 ℃ to 60 ℃. The 3 cycles were repeated nearly 40 times using thermal cycler leading to the formation of millions of copies of a single short segment of DNA from one sample. These cDNA genes encoding HA and NA were selected for the utilization in the construction of lipid nanoparticles mRNA vaccine containing HA and NA mRNA encoding genes of the Swine and Avian flu using invitro transcription technique. The procedure were performed using the invitro MEGAscript T7 Transcription Kit Plus, purchased from Invitrogen, USA; as well as it was operated according to the manufacture instructions. Briefly 10 µg of mRNA transcripts were generated per 1µg template DNA exploiting MEGAscript T7 Transcription Kit. The frozen reagents were thawing, mixed and centrifuged in a gyrator shaker (Corning gyrator shaker, Japan) for 3 minutes at 300 rpm. The enzymes and nucleotides were kept on ice. On the other side, the reaction buffer was maintained at room temperature. Furthermore, the reaction mixture was prepared at room temperature, and incubated at 37 ̊C (Sanyo aerobic incubator, Japan), pH 7.4 for 3 hours. Finally the template DNA was separated using 2 U of DNAase; as well as protein impurities were removed utilizing 3 U proteinase K. The reaction was stopped by adding 2 U 0.5 EDTA at pH 8.5 then the incubation of the reaction mixture at 65 ̊C for 10 minutes. The purification of HA and NA mRNAs was made using reversed phase high performance liquid chromatography (RP-HPLC); as well as the purification was confirmed through Northern blot technique. The mRNAs were then enclosed with solid lipid nanoparticles [LNPs] (20 mcg/ml of dimethyl dioctadecyl ammonium bromide lipid [DDAB]) with size 70 nm prepared using hot microemulsion technique. The test LNP-mRNA vaccine formulation was equipped with a narrow size distribution as its polydispersity index (PDI) was 0.31. On the other hand, It possessed high zeta potential of +28 mV. Lipid nanoparticles -mRNA A ( H1N1,H5N1) immunizing agent was formulated as a parenteral sterile suspension. Each 1 ml dose was formulated to contain 20 mcg mRNA of HA and NA, 20 mcg of DDAB, 0.2 mg of aluminium hydroxide. Each dose; as well as besieged 5 mg of sodium chloride and 0.620 mg of sodium dihydrogen phosphate dihydrate. Immunogenicity was tested in animal models by injecting the purified mRNA in 100 mice by intraperitoneal route of administration. Later, Immunogenicity was well-tried in randomized human clinical trials phases 1/2.
Preclinical Trials (Animal Testing) Procedure:
One hundred mice were challenged with the immunizing agent through intraperitoneal route of administration; while the control group received the placebo through the same route of administration. After 15 days, both groups received graded infectious doses of Swine and Avian flu which ranged from 0.6 to 3 TCID50. The lethal dose was further detected during these protection tests to aid in the determination of the protection power of the test vaccine. The humoral immunity was determined using ELISA. On the other side, the flow cytometry technique using flow cytometer was put-upon to determine the cell mediated immunity through counting the different types of T lymphocytes specific to HA and NA antigens. The percentage of live and killed animal models were recorded during these protection tests to aid in the determination of immunogenicity and the efficacy of the test immunizing agent. As well as, The outcomes were compared with reference standard to assess the relative bioavailability and potency of the test vaccine. Finally, acute toxicity of immunizing agent was estimated using up and down method for assay of acute toxicity.
Randomized Human Clinical Trials Phases 1/2 Procedure:
One hundred human volunteers (test group) with different ages (5-60 years old) were challenged with the test vaccine containing 20 mcg mRNA of HA and NA through intramuscular injection; whereas the control group included 100 candidates who received the placebo intramuscularly. After 28 days, booster doses were given to all participants in this type of protection tests.
Both groups were exposed to graded infectious doses (0.6-3 TCID50) of Swine and Avian flu through respiratory droplets transmission . Blood samples( 5-10 ml) were withdrawn using injection needle (5 µm in diameter) from all participants in the randomized human clinical trials phases 1/2. These blood samples were further subjected to ELISA and flow cytometry techniques to determine the immunogenicity of the test vaccine. On the other hand, Graded quantities of serum (1/1280-1/10) from immunized individuals were transferred to normal volunteers who were then challenged with graded doses (0.6-3 TCID50) of the infectious agent of Swine and Avian flu. The highest dilution of serum efficacious at immunizing 50% of participants in this protection test (i.e. ED50%) was ascertained as a reference point of the efficaciousness of the vaccine. The LD50%, as well as protection power of immunizing agent were determined through these protection tests.
Determination of Humoral Immunity of LNP-mRNA H1N1, H5N1 Flu Vaccine:
ELISA was exploited for detection of neutralizing antibodies. Procedures were applied using ELISA LumiKineTM Xpress 2.0 kits purchased from InvivoGen Europe, France. LumiKineTM Xpress 2.0 kits were sandwich ELISAs that required only a single 2 hour incubation at 37 ̊C (Sanyo aerobic incubator, Japan) and pH 7.3. The procedures of ELISA were followed in accordance with ELISA kit manufacturer. Ten mcg/ml HA and NA Antigens were connected to the well bottom. Antibodies in serum of patient were attached to antigen. 5 µg/ml antibodies to human IgG were connected to patient's IgG, the antibodies to human IgG were enzyme-conjugated (5 mcg/ml Horseradish per-oxidase enzyme). 10 µl substrate for the enzyme was added, which changed color when acted upon by the enzyme. Enzyme activity was calculated by adding the substrate for the enzyme and estimating the color reaction in a UV spectrophotometer (UV1600PC, China) at 450nm wavelength.
Estimation of Cell Mediated Immunity Using Flow Cytometer:
Invitrogen Attune Cytpix flow cytometer purchased from Invitrogen, USA was utilized for counting T lymphocytes. A monoclonal antibody tagged with a fluorescent Rhodamine dye interacted with a cell. As the cell passed down the tube, ultraviolet light caused the dye to fluoresce and a sensor counted the cell. HA and NA mRNA vaccine-specific CD+4 and CD+8 T cells were detected using an Invitrogen Attune Cytpix flow cytometer. In this experiment, a monoclonal antibody was used to tag the patient's cells. These antibodies were directed against cells-specific proteins (e.g. CD4 protein when measuring the number of T helper cells). Fluorescent dyes like rhodamine and fluorescein were used to tag monoclonal antibodies. The laser beam travelled through individual cells, causing them to glow. Using a device called as a Fluorescence Activated Cell Sorter, the fluorescence was evaluated (FACS).
Figure 1.
It depicts the purification of mRNA transcripts of HA using Northern blot technique. The degree of purity was approximately 63%.
Figure 1.
It depicts the purification of mRNA transcripts of HA using Northern blot technique. The degree of purity was approximately 63%.
Figure 2.
It shows 3D structure of NA of Avian flu which elicited the production of neutralizing antibodies in the present study. The 3D structure was designed using SWISS-MODEL software.
Figure 2.
It shows 3D structure of NA of Avian flu which elicited the production of neutralizing antibodies in the present study. The 3D structure was designed using SWISS-MODEL software.
Figure 3.
It displays 3D structure of HA of Swine flu which evoked the manufacture of neutralizing antibodies in the attendant work. The 3D structure was configured using SWISS-MODEL software.
Figure 3.
It displays 3D structure of HA of Swine flu which evoked the manufacture of neutralizing antibodies in the attendant work. The 3D structure was configured using SWISS-MODEL software.
STATISTICS
All cultures were conducted in triplets. Their presentation was by means and standard deviation. One way analysis of variance (p value≤.05) was used as means for performing statistical analysis and also, statistical analysis was based on excel-spreadsheet-software. The F test was used in the present study.
Figure 4.
Determination of antibody titers to NA antigen using ELISA. Potent protect-ant antibodies to NA were elicited due to the previous administration of Swine and Avian bivalent LNP-mRNA flu vaccine.
Figure 4.
Determination of antibody titers to NA antigen using ELISA. Potent protect-ant antibodies to NA were elicited due to the previous administration of Swine and Avian bivalent LNP-mRNA flu vaccine.
Figure 5.
Estimation of antibody titers to NA using ELISA after administration of the test mRNA vaccine during randomized human clinical trials phases 1/2. Novel potent neutralizing antibodies to NA were detected.
Figure 5.
Estimation of antibody titers to NA using ELISA after administration of the test mRNA vaccine during randomized human clinical trials phases 1/2. Novel potent neutralizing antibodies to NA were detected.
RESULTS
Two groups( test, negative control) of mice animal models were included in the present study. Each group consisted of 100 mice. The test bivalent mRNA Swine and Avian flu vaccine were given to the test groups though intraperitoneal route of administration; while the negative control groups were administered the placebo intraperitoneally. One month later, repeated booster doses of the test vaccine and placebo were received by the test and negative control groups respectively. Both groups were challenged with the infectious agent through intranasal route of transmission after 45 days after the beginning of the first vaccine dose. The Immunogenicity testing revealed that about 63%± 1% animal models were killed. On the other hand randomized human clinical trials phases 1/2 revealed that nearly 60%± 3% of volunteers were protected from obtaining the lethal Swine or Avian flu infectious diseases. The count of killed animal models during preclinical trials phases reached 79 after receiving the placebo and infectious dose which ranged from 0.6-3 TCID50; whereas the lethal dose was detected to exceed 5 TCID50. On the other side, during the randomized human clinical trials phases 1/2, only 69 candidates from the negative control group who received the placebo were manifested with lower respiratory infections when they were subjected to 0.6-3 TCID50 infectious doses of the infectious agents (Swine, Avian flu). No symptoms of respiratory infections were detected at infectious doses which ranged from 0.1 to 0.5 TCID50 in randomized human clinical trials phases 1/2. The count of helper lymphocytes type 2 ( TH2) was predominant in participants who received the test mRNA vaccine against Avian and Swine flu as indicated through Table 2. On the other hand, Table 1 revealed that CD+4 type 2 lymphocytes were major prophylactic defence during preclinical trials phases. Potent neutralizing antibodies to NA were detected to play the essential role in the prevention of infection during animal testing as indicated in figure 4. As well as during the randomized human clinical trials phases 1/2, potent prophylactic antibodies to NA were detected as confirmed in figure 5. The counts of CD+8 lymphocytes were negligible in clinical trials phases 1/2 as well as during animal testing of immunogenicity of the test vaccine. The 3D structure of HA and NA antigens were shown in figures 3 and 2, respectively. Slight pain and redness at the site of vaccine injection as well as mild fever were detected as adverse effects during immunogenicity testing in all preclinical trials phases and randomized human clinical trials phases 1/2. Figure 1 shows the purification of mRNA transcripts of HA and NA using Northern blot technique. During the randomized human clinical trials phases 1/2, the determination of passive immunizes against Avian and Swine flu infectious diseases were achieved using preformed antibodies separated from previously infected patients. It was found that 1/40 HA antibody titers were detected to be the highest dilution which prevented the infections with Swine flu; whereas Avian flu infections were hindered using 1/160 HA antibody titers as indicated in Table 5. Antibodies which were produced after the administration of mRNA vaccine were of IgM2 and IgG1 types. No secretory IgA antibodies were detected in samples collected from nasal or oral swabs after the challenge with the booster test vaccine doses. As shown in Table 3 and 4, Potent neutralizing immunoglobulins to HA were detected using ELISA to be the major contributor in the prevention of infectious diseases of Influenza A H1N1 and H5N1 strains.
Table 1.
The count of T lymphocytes in animal testing ( preclinical trials) phases:.
Table 2.
The investigation of T lymphocytes count during randomized human clinical trials phases 1/2:
Table 2.
The investigation of T lymphocytes count during randomized human clinical trials phases 1/2:
Table 3.
Assessment of antibody titers to HA antigen using ELISA in preclinical trials stages at P value ≤ 0.05:
Table 3.
Assessment of antibody titers to HA antigen using ELISA in preclinical trials stages at P value ≤ 0.05:
Table 4.
Compartmentalization of antibody titers to HA antigen using ELISA in randomized human clinical trials stages 1/2 at P values ≤ 0.01:
Table 4.
Compartmentalization of antibody titers to HA antigen using ELISA in randomized human clinical trials stages 1/2 at P values ≤ 0.01:
Table 5.
Detection of passive immunity in protection tests using ELISA technique after injection of different concentrations of preformed antibody titers to HA antigens during randomized human clinical trials phases 1/2:
Table 5.
Detection of passive immunity in protection tests using ELISA technique after injection of different concentrations of preformed antibody titers to HA antigens during randomized human clinical trials phases 1/2:
DISCUSSION
Swine (H1N1) and Avian flu (H5N1) causes outbreaks globally every winter. The present study was concerned with the development of LNP-mRNA bivalent vaccine containing HA and NA antigens of Influenza AH1N1 and Influenza A H5N1. During the preclinical trials, the protection power of the test vaccine estimated through protection tests reached around 63%; while it was about 60% in randomized human clinical trials phases 1/2. The test vaccine evoked humoral immunity during the immunogenicity and protection tests performed through the induction of potent neutralizing antibodies to HA and NA surface protein antigens in Swine and Avian flu. The humoral immunity was detected using ELISA during the animal testing stage as well as the randomized human clinical trials phases 1/2. TH2 ( CD+4 Type II) lymphocytes was observed to be the prototype of the cell mediated immunity evoked during protection tests. TH2 lymphocytes mediated the manufacture of neutralizing antibodies to NA and HA antigens. The major types of Immunoglobulins were were found to be IgM2 after the first exposure to the infectious agents. On the other side, they were IgG1type following the repeated exposure to the same infectious agent. It was detected that no secretory IgA immunoglobulins were produced due to absence of oral and/or nasal route of administration of the test vaccine. Only IgA antibodies are produced if the test vaccine was given orally or intranasally. On the other hand the cell mediated immunity was detected to be weakly stimulated via the test vaccine. Few counts of T lymphocytes (helper and cytotoxic T lymphocytes) were detected using flow cytometer apparatus. The booster dose of the test vaccine in addition to AL(OH)3 improved the immunogenicity and efficacy during randomized human clinical trials phases 1/2 approximately 3%, due to enhancing the production of neutralizing antibodies to NA and HA surface protein antigens of Influenza A H1N1 and Influenza A H5N1. According to Jordan K et al, 2023 study, the current quadrivalent Flu vaccine leaded to overall 70% immunogenicity;[18] while in the present study, the test vaccine leaded to nearly 60% immunogenicity. No herd immunity was obtained from the the test vaccine, owing to the deficiency of the secretion of secretory IgA antibodies in GIT and/or RT. The limits of the present study comprised low sample size. In a comparison with the current flu vaccines in the market, the test vaccine was found to be recommended to given to ages over 2 years; while the current flu vaccines could be utilized in ages above 6 months.[19]
CONCLUSION
The present study was a promising one, since the evolution of novel LNP-mRNA bivalent Swine and Avian flu vaccine occurred. The outbreaks of these infectious agents were detected to be diminished during the current work.
Funding
This study was funded by the single author, Prof. Mohammed Kassab.
Data availability
Data used was provided within the manuscript.
Consent for publication
The author give the permission to the publisher the right to publish the study.
Consent to participate
All informed consents were obtained from all the participants in the present study.
Ethical consideration
All pertinent national, institutional, and/or worldwide guidelines for the care and use of humans and animals were postdated in the current study. The ethical committee for human and animal handling at Cairo University (ECAHCU), the pharmacy faculty at the University of Cairo, Egypt, and the local authorities approved all procedures used in the study, including those involving humans and animals, in accordance with the Weatherall report's recommendations (approval number PXV359/2023). Every attempt was made to reduce the number of people and animals used in the study, as well as their suffering. Randomized human clinical trial registration number CTX628/2023 was obtained and authorized by EDA.
Conflict of interest and competing interests
There are no conflict of interests and competing interests.
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