Version 1
: Received: 16 May 2024 / Approved: 17 May 2024 / Online: 17 May 2024 (05:43:32 CEST)
How to cite:
Fernandes, B.; Caligiorne, R. B.; da Silva, T. A. M.; Campolina, M. M.; La Santrer, E. R. R.; Santiago, I. F.; Assunçao, C. B.; Souza, K. S. M. D.; Valerio, A. D.; Figueiredo, S. M. D.; Johann, S. Exploring Virulence Factors and Antifungal Susceptibility in Sporothrix Isolates: A Comparative Study between Feline and Human Hosts. Preprints2024, 2024051151. https://doi.org/10.20944/preprints202405.1151.v1
Fernandes, B.; Caligiorne, R. B.; da Silva, T. A. M.; Campolina, M. M.; La Santrer, E. R. R.; Santiago, I. F.; Assunçao, C. B.; Souza, K. S. M. D.; Valerio, A. D.; Figueiredo, S. M. D.; Johann, S. Exploring Virulence Factors and Antifungal Susceptibility in Sporothrix Isolates: A Comparative Study between Feline and Human Hosts. Preprints 2024, 2024051151. https://doi.org/10.20944/preprints202405.1151.v1
Fernandes, B.; Caligiorne, R. B.; da Silva, T. A. M.; Campolina, M. M.; La Santrer, E. R. R.; Santiago, I. F.; Assunçao, C. B.; Souza, K. S. M. D.; Valerio, A. D.; Figueiredo, S. M. D.; Johann, S. Exploring Virulence Factors and Antifungal Susceptibility in Sporothrix Isolates: A Comparative Study between Feline and Human Hosts. Preprints2024, 2024051151. https://doi.org/10.20944/preprints202405.1151.v1
APA Style
Fernandes, B., Caligiorne, R. B., da Silva, T. A. M., Campolina, M. M., La Santrer, E. R. R., Santiago, I. F., Assunçao, C. B., Souza, K. S. M. D., Valerio, A. D., Figueiredo, S. M. D., & Johann, S. (2024). Exploring Virulence Factors and Antifungal Susceptibility in<em> Sporothrix </em>Isolates: A Comparative Study between Feline and Human Hosts. Preprints. https://doi.org/10.20944/preprints202405.1151.v1
Chicago/Turabian Style
Fernandes, B., Sonia Maria de Figueiredo and Susana Johann. 2024 "Exploring Virulence Factors and Antifungal Susceptibility in<em> Sporothrix </em>Isolates: A Comparative Study between Feline and Human Hosts" Preprints. https://doi.org/10.20944/preprints202405.1151.v1
Abstract
Sporotrichosis’ clinical manifestations, lesions in the cutaneous or subcutaneous tissues, it is caused by dimorphic fungi of the Sporothrix complex, is a cosmopolitan mycosis that affects humans and animals. This study aimed to identify prevalent species in Minas Gerais, Brazil, and characterize virulence profiles and antifungal susceptibility associated with Sporothrix isolates. Isolates were identified using macro and micro-morphological traits as well molecular biology techniques. Antifungal susceptibility was determined using the Minimal Inhibitory Concentration test, while, virulence factors such as growth at different temperatures, biofilm formation, melanin production, and urease activity were assessed. Our findings revealed S. brasiliensis as a predominant species isolated. Urease activity was a crucial factor associated with growth at 50°C in S. brasiliensis isolates, highlighting their high thermotolerance. Furthermore, strains isolated from humans produced a more robust biofilm compare to those from felines. Strains of S. brasiliensis isolated from Felis catus exhibited faster melanin production, decreased susceptible to antifungal agents tested, and superior growth capacity at 50°C to strains isolated from humans. Therefore, our study highlights the prevalence of S. brasiliensis causing sporotrichosis in both human and felines. However, it underscores the circulation of different strains, with varying levels of virulence and susceptibility to antifungal agents.
Keywords
Sporothrix; sporothricosis; virulence profile; antifungal susceptibility; species identification.
Subject
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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