Adult human brain tissue cultures to study neuroHIV

How to cite: Van Duyne, R.; Irollo, E.; Lin, A.; Johnson, J.A.; Guillem, A.M.; O’Brien, E.V.; Merja, L.; Nash, B.; Jackson, J.G.; Sarkar, A.; Klase, Z.A.; Meucci, O. Adult human brain tissue cultures to study neuroHIV. Preprints 2024, 2024041300. https://doi.org/10.20944/preprints202404.1300.v1 Van Duyne, R.; Irollo, E.; Lin, A.; Johnson, J.A.; Guillem, A.M.; O’Brien, E.V.; Merja, L.; Nash, B.; Jackson, J.G.; Sarkar, A.; Klase, Z.A.; Meucci, O. Adult human brain tissue cultures to study neuroHIV. Preprints 2024, 2024041300. https://doi.org/10.20944/preprints202404.1300.v1

Cite

Export citation file: Bib TeX | RIS

MDPI and ACS Style

Van Duyne, R.; Irollo, E.; Lin, A.; Johnson, J.A.; Guillem, A.M.; O’Brien, E.V.; Merja, L.; Nash, B.; Jackson, J.G.; Sarkar, A.; Klase, Z.A.; Meucci, O. Adult human brain tissue cultures to study neuroHIV. Preprints 2024, 2024041300. https://doi.org/10.20944/preprints202404.1300.v1

APA Style

Van Duyne, R., Irollo, E., Lin, A., Johnson, J.A., Guillem, A.M., O’Brien, E.V., Merja, L., Nash, B., Jackson, J.G., Sarkar, A., Klase, Z.A., & Meucci, O. (2024). Adult human brain tissue cultures to study neuroHIV. Preprints. https://doi.org/10.20944/preprints202404.1300.v1

Chicago/Turabian Style

Van Duyne, R., Zachary A. Klase and Olimpia Meucci. 2024 "Adult human brain tissue cultures to study neuroHIV" Preprints. https://doi.org/10.20944/preprints202404.1300.v1

Abstract

HIV-associated neurocognitive disorders (HAND) persist under antiretroviral therapy as a complex pathology that has been difficult to study in cellular and animal models. Therefore, we generated an ex vivo human brain slice model of HIV-1 infection from surgically resected adult brain tissue. Brain slice cultures processed for flow cytometry showed >90% viability of dissoci-ated cells within the first three weeks in vitro, with parallel detection of astrocyte, myeloid, and neuronal populations. Neurons within brain slices showed stable dendritic spine density and mature spine morphologies in the first weeks in culture, and they generated detectable activity in multi-electrode arrays. We infected cultured brain slices using patient-matched CD4+ T-cells or monocyte-derived macrophages (MDMs) that were exposed to a GFP-expressing R5-tropic HIV-1 in vitro. Infected slice cultures expressed viral RNA and developed a spreading infection up to 9-days post-infection, which were significantly decreased by antiretrovirals. We also detected infected myeloid cells and astrocytes within slices and observed minimal effect on cellular via-bility over time. Overall, this human-centered model offers a promising resource to study the cel-lular mechanisms contributing to HAND (including antiretroviral toxicity, substance use, and aging), infection of resident brain cells, and new neuroprotective therapeutics.

Keywords

HIV-associated neurocognitive disorders (HAND); human brain organotypic slice cultures; HIV-1 infection; dendritic spine analysis; microelectrode array

Subject

Medicine and Pharmacology, Neuroscience and Neurology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF Download Supplementary

Comments (0)

Not displayed online.

Importance: How significant is the paper to the field?

Outstanding/highlight paper

Significant contribution

Incremental contribution

No contribution

I am not qualified to judge

Soundness of evidence/arguments presented:

Conclusions well supported

Most conclusions supported (minor revision needed)

Incomplete evidence (major revision needed)

Hypothesis, unsupported conclusions, or proof-of-principle

I am not qualified to judge

Please leave your comment to this article below.

Mathematical equations can be typed in either LaTeX formats \\[ ... \\] or $$ ... $$, or MathML format <math> ... </math>. Try the LaTeX or MathML example.

Type equation:

Preview:

Copy equation into message below

Close menu

Please click a symbol to insert it into the message box below:

Please enter the link here:

Please enter a valid URL

Optionally, you can enter text that should appear as linked text:

Copy link into message below

Close menu

Please enter or paste the URL to the image here (please only use links to jpg/jpeg, png and gif images):

Please enter a valid URL

Copy image into message below

Close menu

Type author name or keywords to filter the list of references in this group (you can add a new citation under Bibliography):

No existing citations in Discussion Group

Wikify editor is a simple editor for wiki-style mark-up. It was written by MDPI for Sciforum in 2014. The rendering of the mark-up is based on Wiky.php with some tweaks. Rendering of mathematical equations is done with MathJax. Please send us a message for support or for reporting bugs.

Close menu

Please declare any conflicts of interest you may have with this paper, financial or otherwise.

I do not have a conflict of interest

I would like to declare a conflict of interest

Display my name on the website Send my name and email address to the authors

Captcha

Renew

I accept the following conditions:

Comments must follow the standards of professional discourse and should focus on the scientific content of the article. Insulting or offensive language, personal attacks and off-topic remarks will not be permitted. Comments must be written in English. Preprints reserves the right to remove comments without notice. Readers who post comments are obliged to declare any competing interests, financial or otherwise.

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)

* All users must log in before leaving a comment

what’s this?

Add a record of this review to Publons to track and showcase your reviewing expertise across the world’s journals.

Downloads 0

Comments 0

Metrics 0

Get PDF Supplementary Files Cite Share 0

Bookmark BibSonomy Mendeley Reddit Delicious

Alerts

Notify me about updates to this article or when a peer-reviewed version is published.

Preprints.org is a free preprint server subsidized by MDPI in Basel, Switzerland.

MDPI Initiatives

SciProfiles
Sciforum
Encyclopedia
MDPI Books
Scilit
Proceedings
JAMS

Important links

How it Works
Advisory Board
FAQ
Friendly Journals
Instructions for Authors
About
Statistics

Choose the area that interest you and we will send you notifications of new preprints at your preferred frequency.

Disclaimer

We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.

RSS feed for

Preprints: The Multidisciplinary Preprint Platform

Paste the URL in your RSS reader:

https://www.preprints.org/rss

If you want to subscribe our website in subject-wide, you could find the URL by choosing in the following:

https://www.preprints.org/rss

Please note that by using ScienceDirect RSS feeds, you agree to our Terms of Use and Privacy Policy.