Version 1
: Received: 17 April 2024 / Approved: 18 April 2024 / Online: 18 April 2024 (07:51:19 CEST)
How to cite:
Nokhova, A.R.; Dubovitskiy, N.A.; Derko, A.A.; Khozyainova, A.A.; Kurskaya, O.G.; Shestopalov, A.M.; Sharshov, K.A. Genetic Characteristics of Anelloviruses Detected in Individual Viromes of Children with Acute Respiratory Infection Using the Metagenomic Approach. Preprints2024, 2024041211. https://doi.org/10.20944/preprints202404.1211.v1
Nokhova, A.R.; Dubovitskiy, N.A.; Derko, A.A.; Khozyainova, A.A.; Kurskaya, O.G.; Shestopalov, A.M.; Sharshov, K.A. Genetic Characteristics of Anelloviruses Detected in Individual Viromes of Children with Acute Respiratory Infection Using the Metagenomic Approach. Preprints 2024, 2024041211. https://doi.org/10.20944/preprints202404.1211.v1
Nokhova, A.R.; Dubovitskiy, N.A.; Derko, A.A.; Khozyainova, A.A.; Kurskaya, O.G.; Shestopalov, A.M.; Sharshov, K.A. Genetic Characteristics of Anelloviruses Detected in Individual Viromes of Children with Acute Respiratory Infection Using the Metagenomic Approach. Preprints2024, 2024041211. https://doi.org/10.20944/preprints202404.1211.v1
APA Style
Nokhova, A.R., Dubovitskiy, N.A., Derko, A.A., Khozyainova, A.A., Kurskaya, O.G., Shestopalov, A.M., & Sharshov, K.A. (2024). Genetic Characteristics of Anelloviruses Detected in Individual Viromes of Children with Acute Respiratory Infection Using the Metagenomic Approach. Preprints. https://doi.org/10.20944/preprints202404.1211.v1
Chicago/Turabian Style
Nokhova, A.R., Alexander M. Shestopalov and Kirill A. Sharshov. 2024 "Genetic Characteristics of Anelloviruses Detected in Individual Viromes of Children with Acute Respiratory Infection Using the Metagenomic Approach" Preprints. https://doi.org/10.20944/preprints202404.1211.v1
Abstract
More than 20 years after the discovery of the first anelloviruses (AVs), they still remain a mysterious group of viruses. To date, there is no clear understanding of their impact on the host organism, although information is emerging about their participation in various pathologies. However, it is well known that AVs are an important component of the human virome. In the current study we assessed the abundance of AVs in viromes from four nasal and pharyngeal samples from children with respiratory symptoms using a metagenomic approach. It is worth noting that three patients had known respiratory viruses and one did not have any. Regarding AVs, they were detected in all samples, namely Torque teno mini virus (TTMV). The greatest abundance and diversity of AVs (Torque teno virus (TTV), TTMV and Torque teno midi virus (TTMDV)) were shown in a diseased patient with an absence of respiratory viruses, which may indicate the potential involvement of AVs in the maintenance of respiratory symptoms. An activated immune system to combat the main pathogens, against the background of which the number of AVs decreases, is discussed as a possible reason for the reduced diversity of anellome in other patients. We also compared the results of the metagenomic approach and qPCR for major respiratory viruses, which were in agreement. In addition, metagenomic sequencing made it possible to obtain additional data on viral genomes in order to establish their taxonomic identification and characterize individual viral profiles in patients from the Siberian part of Russia.
Keywords
viral metagenomics; respiratory virome; torque teno virus; torque teno mini virus; torque teno midi virus; anelloviruses; human anellovirus; Anelloviridae; anellome
Subject
Biology and Life Sciences, Virology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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