Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Microbiome diversity in raw milk from Comisana and Lacaune sheep and microbial evolution during artisanal Pecorino-like cheese

Version 1 : Received: 15 April 2024 / Approved: 17 April 2024 / Online: 17 April 2024 (12:21:08 CEST)

How to cite: Luziatelli, F.; Abou Jaudé, R.; Melini, F.; Melini, V.; Ruzzi, M. Microbiome diversity in raw milk from Comisana and Lacaune sheep and microbial evolution during artisanal Pecorino-like cheese. Preprints 2024, 2024041108. https://doi.org/10.20944/preprints202404.1108.v1 Luziatelli, F.; Abou Jaudé, R.; Melini, F.; Melini, V.; Ruzzi, M. Microbiome diversity in raw milk from Comisana and Lacaune sheep and microbial evolution during artisanal Pecorino-like cheese. Preprints 2024, 2024041108. https://doi.org/10.20944/preprints202404.1108.v1

Abstract

“Pecorino” is a typical semi-hard cheese obtained with raw or heat-treated sheep milk using procedures to valorize the raw material's chemical and microbiological properties. In the present study, using a high throughput method of 16S rRNA gene sequencing, we assessed the evolution of the microbiome composition from milk to Pecorino cheese in artisanal processes using milk from Comisana and Lacaune sheep breeds. The comparative analysis of the bacterial com-munity composition revealed significant differences in the presence and abundance of specific taxa in the milk microbiome of the Comisana and Lacaune breeds. NGS analysis also revealed differences in the curd microbiome related to dairy farming practices, which have a relevant effect on the final structure of the Pecorino-cheese microbiome.

Keywords

Comisana breed; Lacaune breed; sheep milk; microbiome; Next-Generation Sequencing (NGS); Pecorino cheese

Subject

Biology and Life Sciences, Food Science and Technology

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