Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

GreenTray®, a TIS Bioreactor for Plant Micropropagation and Abiotic or Biotic Stress Bioassays

Version 1 : Received: 10 April 2024 / Approved: 11 April 2024 / Online: 11 April 2024 (11:12:04 CEST)

How to cite: Dolcet-Sanjuan, R.; Casanovas, M.; Franquesa, S.; Alsina, E.; Carrasco-Cuello, F.; Torres, E.; Rufat, J.; Solsona, C.; Teixido, N. GreenTray®, a TIS Bioreactor for Plant Micropropagation and Abiotic or Biotic Stress Bioassays. Preprints 2024, 2024040793. https://doi.org/10.20944/preprints202404.0793.v1 Dolcet-Sanjuan, R.; Casanovas, M.; Franquesa, S.; Alsina, E.; Carrasco-Cuello, F.; Torres, E.; Rufat, J.; Solsona, C.; Teixido, N. GreenTray®, a TIS Bioreactor for Plant Micropropagation and Abiotic or Biotic Stress Bioassays. Preprints 2024, 2024040793. https://doi.org/10.20944/preprints202404.0793.v1

Abstract

The temporary immersion system bioreactor named GreenTray®[2], [3]presents advantages over the existing ones. Firstly, there is no need to use forceps to move the shoots or plantlets in or out of the recipient, neither the use of scalpel to divide them. Secondly, the basis of the shoot can remain in the GreenTray® and can sprout again in several cycles of growth. These two characteristics reduce the costs in the process of micropropagation. Besides, shoots or plantlets development is enhanced by the aeration of the recipient, thus enriching with CO2 the recipient atmosphere. The chapter describes and provides images and values of interest in (1) the micropropagation process of some commercial Prunus or Pyrus rootstocks; (2) the plantlet growth under autotrophic conditions; and (2) the inoculation of plants with pathogens that reproduce in vitro the symptoms observed in vivo, using the GreenTray® bioreactor.

Keywords

GreenTray®; TIS; bioreactor; micropropagation; Biotic and Abiotic Stress Agents Bioassays; Prunus; Pyrus; Malus; Cannabis

Subject

Biology and Life Sciences, Plant Sciences

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