Rossato, R.C.; Albuquerque, A.L.; Salles, G.R.; Porcionatto, M.A.; Granato, A.E.C.; Ulrich, H.; Santos, M.I.B.D.; Pacheco-Soares, C. Neuroprotective Effects of Photobiomodulation and Taurine on SH-SY5Y Cells. Preprints2024, 2024040362. https://doi.org/10.20944/preprints202404.0362.v1
APA Style
Rossato, R.C., Albuquerque, A.L., Salles, G.R., Porcionatto, M.A., Granato, A.E.C., Ulrich, H., Santos, M.I.B.D., & Pacheco-Soares, C. (2024). Neuroprotective Effects of Photobiomodulation and Taurine on SH-SY5Y Cells. Preprints. https://doi.org/10.20944/preprints202404.0362.v1
Chicago/Turabian Style
Rossato, R.C., Mariela Ines Batista dos Santos and Cristina Pacheco-Soares. 2024 "Neuroprotective Effects of Photobiomodulation and Taurine on SH-SY5Y Cells" Preprints. https://doi.org/10.20944/preprints202404.0362.v1
Abstract
Abstract: Alzheimer's Disease (AD) is a progressive uncurable neurodegenerative disease affect-ing millions worldwide. Various methods, including drug therapies and light emission diode (LED) irradiation, are promising alternatives for preventing and reducing the rapid progression of neurodegenerative diseases, also stimulating the reconstructing of neural tissue structures. In this study, we evaluated the neuroprotective and restorative effects of taurine combined with LED on human neuroblastoma cells (SH-SY5Y), under oxidative stress condition, a considerable modulator in AD. We evaluated LED at the wavelength of 660 nm and taurine under different concentrations before and after exposing SH-SY5Y cells to different concentrations of hydrogen peroxide (H2O2), assessing mitochondrial activity by the MTT colorimetric test and labeling live cell mitochondria by fluorescence using MitoTracker Orange. Cell viability was also evaluated by the trypan blue exclusion assay, and we verified cellular morphological structures by scan-ning electron microscopy (SEM). It was observed that neuroprotective effects are achieved by both LED and taurine when cells are exposed to them and later are stressed with H2O2. Compar-ing both agents, LED irradiation is sufficient to stimulate cell proliferation, representing an af-fordable candidate to contribute against neurodegeneration.
Biology and Life Sciences, Neuroscience and Neurology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
,
,
