Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Plant Biostimulants Enhance Bud Break in Vitis vinifera Crimson Seedless

Version 1 : Received: 3 April 2024 / Approved: 3 April 2024 / Online: 3 April 2024 (11:56:31 CEST)

How to cite: Venter, N.C.; Avenant, E.; Kotze, T.N.; Hills, P.N.; Moore, J.P. Plant Biostimulants Enhance Bud Break in Vitis vinifera Crimson Seedless. Preprints 2024, 2024040286. https://doi.org/10.20944/preprints202404.0286.v1 Venter, N.C.; Avenant, E.; Kotze, T.N.; Hills, P.N.; Moore, J.P. Plant Biostimulants Enhance Bud Break in Vitis vinifera Crimson Seedless. Preprints 2024, 2024040286. https://doi.org/10.20944/preprints202404.0286.v1

Abstract

The rest-breaking agent, hydrogen cyanamide (HC), can substitute insufficient chill unit accumulation in Vitis vinifera and induce uniform bud-break; however, due to its toxicity it is being banned. In South Africa, red seedless grapes, including V. vinifera Crimson Seedless (CS), are the largest table grape export group; therefore, replacing HC in V. vinifera CS is crucial. This study aimed to confirm the molecular triggers induced by HC and assess the bud-break-enhancing abilities of commercial plant biostimulants. Forced bud-break assay experiments using V. vinifera CS single-node cuttings and a small-scale field trial were performed. Results demonstrated that increased chill unit accumulation (CUA), reduced HC efficacy. Bud-break started between 10 and 20 days after treatment, irrespective of final CUA. The small-scale field trial found that HC3 % resulted in a slightly delayed bud-break rate, whilst biostimulants were similar to the negative control. The treatment of dormant grapevine compound buds with nitric oxide (NO), hydrogen peroxide (H2O2), and hypoxia trigger dormancy-release to a certain extent, supporting the molecular models proposed for HC action. NO, H2O2, and hypoxia, in combination with PBs, may potentially replace HC; however, this needs to be confirmed in future experiments.

Keywords

Vitis vinifera, Crimson Seedless, bud break, plant biostimulant, hypoxia

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

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