Changes in HERVs Expression and Interleukins mRNAs Levels in Trophoblastic Cells Infected by ZIKV

Anderson Luís da Costa; Paula Prieto-Oliveira; Márcia Duarte-Barbosa; Robert Andreata-Santos; Cristina M. Peter; Thamires Prolo; Fernando Antoneli; Maria Isabel V. G. de Carvalho; Ricardo Durães-Carvalho; Marcelo Ribeiro da Silva Briones; Juliana T. Maricato; Paolo M. A. Zanotto; Denis Jacob Machado; Luiz Mario Ramos Janini

doi:10.20944/preprints202404.0232.v1

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preprints.org > biology and life sciences > virology > doi: 10.20944/preprints202404.0232.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Changes in HERVs Expression and Interleukins mRNAs Levels in Trophoblastic Cells Infected by ZIKV

Anderson Luís da Costa

Paula Prieto-Oliveira

Márcia Duarte-Barbosa

Robert Andreata-Santos

Cristina M. Peter

Thamires Prolo

Fernando Antoneli

Maria Isabel V. G. de Carvalho

Ricardo Durães-Carvalho

Marcelo Ribeiro da Silva Briones

Juliana T. Maricato

Paolo M. A. Zanotto

Denis Jacob Machado

Luiz Mario Ramos Janini

Version 1 : Received: 2 April 2024 / Approved: 2 April 2024 / Online: 2 April 2024 (17:29:09 CEST)

How to cite: Costa, A.L.D.; Prieto-Oliveira, P.; Duarte-Barbosa, M.; Andreata-Santos, R.; Peter, C.M.; Prolo, T.; Antoneli, F.; Carvalho, M.I.V.G.D.; Durães-Carvalho, R.; Briones, M.R.D.S.; Maricato, J.T.; Zanotto, P.M.A.; Jacob Machado, D.; Janini, L.M.R. Changes in HERVs Expression and Interleukins mRNAs Levels in Trophoblastic Cells Infected by ZIKV. Preprints 2024, 2024040232. https://doi.org/10.20944/preprints202404.0232.v1 Costa, A.L.D.; Prieto-Oliveira, P.; Duarte-Barbosa, M.; Andreata-Santos, R.; Peter, C.M.; Prolo, T.; Antoneli, F.; Carvalho, M.I.V.G.D.; Durães-Carvalho, R.; Briones, M.R.D.S.; Maricato, J.T.; Zanotto, P.M.A.; Jacob Machado, D.; Janini, L.M.R. Changes in HERVs Expression and Interleukins mRNAs Levels in Trophoblastic Cells Infected by ZIKV. Preprints 2024, 2024040232. https://doi.org/10.20944/preprints202404.0232.v1

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MDPI and ACS Style

Costa, A.L.D.; Prieto-Oliveira, P.; Duarte-Barbosa, M.; Andreata-Santos, R.; Peter, C.M.; Prolo, T.; Antoneli, F.; Carvalho, M.I.V.G.D.; Durães-Carvalho, R.; Briones, M.R.D.S.; Maricato, J.T.; Zanotto, P.M.A.; Jacob Machado, D.; Janini, L.M.R. Changes in HERVs Expression and Interleukins mRNAs Levels in Trophoblastic Cells Infected by ZIKV. Preprints 2024, 2024040232. https://doi.org/10.20944/preprints202404.0232.v1

APA Style

Costa, A.L.D., Prieto-Oliveira, P., Duarte-Barbosa, M., Andreata-Santos, R., Peter, C.M., Prolo, T., Antoneli, F., Carvalho, M.I.V.G.D., Durães-Carvalho, R., Briones, M.R.D.S., Maricato, J.T., Zanotto, P.M.A., Jacob Machado, D., & Janini, L.M.R. (2024). Changes in HERVs Expression and Interleukins mRNAs Levels in Trophoblastic Cells Infected by ZIKV. Preprints. https://doi.org/10.20944/preprints202404.0232.v1

Chicago/Turabian Style

Costa, A.L.D., Denis Jacob Machado and Luiz Mario Ramos Janini. 2024 "Changes in HERVs Expression and Interleukins mRNAs Levels in Trophoblastic Cells Infected by ZIKV" Preprints. https://doi.org/10.20944/preprints202404.0232.v1

Abstract

Zika virus (ZIKV) is a notable arbovirus with maternal, sexual, and TORCH-related transmission capabilities. After 2015, Brazil had the highest number of ZIVK-infected pregnant women who lost their babies or delivered them with Congenital ZIKV Syndrome (CZS). ZIKV infection has been linked to variations in endogenous retroviruses (ERVs) and interleukins within the placenta. ERVs, remnants of ancient viral infections integrated into the genome, include syncytin receptors crucial for placental development. Interleukins, immune response regulators, aid immune tolerance and support syncytial structure development in the placenta, where syncytin receptors facilitate vital cell-to-cell fusion events. Since ZIKV can infect trophoblast cells, we investigated the relationship between ZIKV infections and ERV and interleukin modulations. Investigating the impact of ZIKV on trophoblast cells, we examined two cell types (BeWo and HTR8) infected with ZIKV-MR766 (African) and ZIKV-IEC-Paraíba (Asia-Brazilian) using Taqman and RT2 Profiler PCR Array assays. While early ZIKV infection (24-48 hours) did not induce differential ERV and interleukin expression, future studies involving additional trophoblastic cell lineages and extended infection timelines will illuminate the dynamic interplay between ZIKV, HERVs, interleukins, and cytokines essential for placental formation and function.

Keywords

ERV; ZIKV; Interleukins; Citokines; Trophoblast Cells

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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