Version 1
: Received: 1 April 2024 / Approved: 1 April 2024 / Online: 1 April 2024 (14:39:31 CEST)
How to cite:
Cheng, M.; Yang, M.; Mu, X.; Jing, Z.; Du, M.; Jiao, J.; Wang, Z. The Isolation, Purification and Active Mechanism of Sea Cucumber Bone-Strengthening Functional Peptides. Preprints2024, 2024040096. https://doi.org/10.20944/preprints202404.0096.v1
Cheng, M.; Yang, M.; Mu, X.; Jing, Z.; Du, M.; Jiao, J.; Wang, Z. The Isolation, Purification and Active Mechanism of Sea Cucumber Bone-Strengthening Functional Peptides. Preprints 2024, 2024040096. https://doi.org/10.20944/preprints202404.0096.v1
Cheng, M.; Yang, M.; Mu, X.; Jing, Z.; Du, M.; Jiao, J.; Wang, Z. The Isolation, Purification and Active Mechanism of Sea Cucumber Bone-Strengthening Functional Peptides. Preprints2024, 2024040096. https://doi.org/10.20944/preprints202404.0096.v1
APA Style
Cheng, M., Yang, M., Mu, X., Jing, Z., Du, M., Jiao, J., & Wang, Z. (2024). The Isolation, Purification and Active Mechanism of Sea Cucumber Bone-Strengthening Functional Peptides. Preprints. https://doi.org/10.20944/preprints202404.0096.v1
Chicago/Turabian Style
Cheng, M., Jian Jiao and Zhenyu Wang. 2024 "The Isolation, Purification and Active Mechanism of Sea Cucumber Bone-Strengthening Functional Peptides" Preprints. https://doi.org/10.20944/preprints202404.0096.v1
Abstract
Sea cucumber peptides are small molecular peptides with biological function obtained from fresh sea cucumber as feedstock, which were hydrolyzed by protease, separated ,and purified. As the main product of sea cucumber refinement, it more accurately expresses the nutritional effect of sea cucumber and has a wide range of potential applications. It has been demonstrated that sea cucumber has a certain value-added effect on bone mineral density regulation, but the differences in bone mineral density regulation of extracts from different parts of sea cucumber and the techniques contributing to the efficient isolation of bone sea cucumber peptides have not been reported. Firstly, the aim of this study was to reveal the differences in osteogenic activities of enzymatic peptides from three major parts of sea cucumber: the body wall, the egg protein, and the intestinal protein. Then, the bone-enhancing sea cucumber peptides were isolated based on the characteristics of the dif-ferences in molecular weight distribution, hydrophilicity, and ionisation degree of sea cucumber, and to explore the possible mechanisms of their action. The findings indicated that sea cucumber peptides extracted from three distinct sites exhibited a dose-dependent enhancement of osteoblast proliferation and differentiation. Upon evaluating the three methods of sea cucumber peptide separation based on molecular weight distribution, hydrophilicity, and ionization, it was observed that the fraction isolated by hydrophobicity and
Keywords
protein, osteoporosis, MC3T3-E1 cells, peptide
Subject
Biology and Life Sciences, Food Science and Technology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
