Version 1
: Received: 29 March 2024 / Approved: 29 March 2024 / Online: 29 March 2024 (13:19:07 CET)
How to cite:
Li, X.; Li, Y.; Zhu, J. Mutations and Differential Transcription of Mating-Type and Pheromone Receptor Genes in Hirsutella sinensis and Natural Cordyceps sinensis. Preprints2024, 2024031854. https://doi.org/10.20944/preprints202403.1854.v1
Li, X.; Li, Y.; Zhu, J. Mutations and Differential Transcription of Mating-Type and Pheromone Receptor Genes in Hirsutella sinensis and Natural Cordyceps sinensis. Preprints 2024, 2024031854. https://doi.org/10.20944/preprints202403.1854.v1
Li, X.; Li, Y.; Zhu, J. Mutations and Differential Transcription of Mating-Type and Pheromone Receptor Genes in Hirsutella sinensis and Natural Cordyceps sinensis. Preprints2024, 2024031854. https://doi.org/10.20944/preprints202403.1854.v1
APA Style
Li, X., Li, Y., & Zhu, J. (2024). Mutations and Differential Transcription of Mating-Type and Pheromone Receptor Genes in Hirsutella sinensis and Natural Cordyceps sinensis. Preprints. https://doi.org/10.20944/preprints202403.1854.v1
Chicago/Turabian Style
Li, X., Yu-Ling Li and Jia-Shi Zhu. 2024 "Mutations and Differential Transcription of Mating-Type and Pheromone Receptor Genes in Hirsutella sinensis and Natural Cordyceps sinensis" Preprints. https://doi.org/10.20944/preprints202403.1854.v1
Abstract
The sexual reproduction of ascomycetes is controlled by the mating-type(MAT) locus. (Pseudo)homothallic reproduction has been hypothesized based on genetic data from Hirsutella sinensis (Genotype #1 of Ophiocordyceps sinensis). In this study, the differential occurrence of the mating-type genes in the MAT1-1 and MAT1-2 idiomorphs, which constitute the genetic control of O. sinensis reproduction, was analyzed in 237 strains and 5 genomes of H. sinensis. The mating-type genes were differentially transcribed in H. sinensis Strain L0106, and the MAT1-2-1 transcript in H. sinensis Strain 1229 was alternatively spliced, with an unspliced intron I containing stop codons; these findings constitute O. sinensis reproduction controls at the transcriptional and coupled transcriptional-translational levels. The occurrence of the mating-type genes and a-/α-pheromone receptor genes differed among the 5 H. sinensis genomes. The differential occurrence and transcription of these genes are inconsistent with self-fertilization under (pseudo)homothallism but suggest self-sterility of H. sinensis, which therefore needs a mating partner to achieve sexual outcrossing under heterothallism or hybridization. Consistent occurrence and transcription of the mating-type genes of both the MAT1-1 and MAT1-2 idiomorphs have been reported in natural and cultivated Cordyceps sinensis, but these findings are insufficient to determine the reproduction mode(s) because of the co-occurrence of >90 fungal species and multiple genomically independent genotypes of O. sinensis. The mutant MAT1-1-1 and α-pheromone receptor transcripts in natural C. sinensis result in the N-terminus or middle-truncated proteins, with changes in hydrophobicity and secondary structures of the proteins, suggesting heterogeneous fungal source(s) of the proteins and hybridization reproduction.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
