Version 1
: Received: 28 March 2024 / Approved: 29 March 2024 / Online: 29 March 2024 (13:50:03 CET)
How to cite:
Mollea, C.; Bosco, F. Fermentation of BSG Syrup with R. mucilaginosa to Produce Carotenoids. Preprints2024, 2024031826. https://doi.org/10.20944/preprints202403.1826.v1
Mollea, C.; Bosco, F. Fermentation of BSG Syrup with R. mucilaginosa to Produce Carotenoids. Preprints 2024, 2024031826. https://doi.org/10.20944/preprints202403.1826.v1
Mollea, C.; Bosco, F. Fermentation of BSG Syrup with R. mucilaginosa to Produce Carotenoids. Preprints2024, 2024031826. https://doi.org/10.20944/preprints202403.1826.v1
APA Style
Mollea, C., & Bosco, F. (2024). Fermentation of BSG Syrup with <em>R. mucilaginosa</em> to Produce Carotenoids. Preprints. https://doi.org/10.20944/preprints202403.1826.v1
Chicago/Turabian Style
Mollea, C. and Francesca Bosco. 2024 "Fermentation of BSG Syrup with <em>R. mucilaginosa</em> to Produce Carotenoids" Preprints. https://doi.org/10.20944/preprints202403.1826.v1
Abstract
A Brewer Spent Grain (BSG), the most abundant by-product of the brewing process, is mainly used in animal feed and biogas production. The aim of the present work is BSG valorization by yeast fermentation to produce carotenoids.
R. mucilaginosa CBS316 was cultured on BSG syrups, supplemented or not with YE and PEP (s-Fsa and Fsa). The biomass and carotenoid production were optimized in flasks and then scaled-up in bioreactor.
In flask tests, the maximum biomass concentration was 5.3 and 6.2 g/L for Fsa and s-Fsa cultures, respectively. The maximum carotenoid production was 833 μg/L in Fsa and 1159 μg/L in s-FSA. Considering that the highest specific productivity value (P 7.16 μg/g*h) was obtained with Fsa syrup, this medium was selected for the process scale-up. In bioreactor tests, the maximum concentrations of biomass (4.29 g/L) and carotenoid (396 ug/L) was reached at 72 hours of fer-mentation, 24 h later than in flask. The total carotenoid produced was 524 and 150 ug in bioreactor and flask cultures, respectively.
The carotenoids production by R. mucilaginosa was obtained both in Fsa and s-Fsa syrups. The results confirm the possibility to use the syrup as it is, Fsa, thus limiting the process costs.
Keywords
Brewery spent grain; R. mucilaginosa; carotenoids; syrup; bioreactor; fermentation
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
