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A peer-reviewed article of this preprint also exists.
This version is not peer-reviewed
Submitted:
26 March 2024
Posted:
26 March 2024
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Target gene | Marker name | Primer name | Sequence (5'-3') | Product size (bp) |
---|---|---|---|---|
Bph6 | B6 | SF | AGGGCCTCTGGCGCTCTAC | 361(-) / 283(+) |
SR | AATGTGAAAGTGCAATTAGAAGGT | |||
RF | ATAGTGAAGTTGAATCCGAAGG | |||
RR | AGTGACTCAGCCTTGTGTTTCG | |||
Bph9 | B9 | IF | ACCATTGTTAGGCAGTTGTTCA | 316(-) / 230(+) |
OR | ATTCGACTCCCTTTCTTGTTATCT | |||
OF | AATGTCGCACCCAGCAGC | |||
IR | CAGCCTCCTGAAGAGATCTTTCA | |||
BPH1/9 | B1/9 | SF | GGCGAGTTGATGAAATAAAGG | 473(-) / 545(+) |
SR | GTTCTTTGCATCGCTGTCTC | |||
RR | TTGCTCCCACCGAAGTCA | |||
RF | AGGGAATGGTGTAGTAGCA | |||
Pigm | GM | OF | TGCTGAACAAGGTGTAGAGGTA | 448(-) / 621(+) |
OR | GCCAGGTCCTACTTCCACAAAA | |||
IF | GTGACATCCAGTCCTACACAATCTAA | |||
IR | CACGGAACTCGTTTTCGTC |
Lines | Genes of pyramiding | Number of Lines | Seedling BPH resistance score | Seedling blast resistance score |
---|---|---|---|---|
Gm69 lines | Bph6+Bph9+Pigm | 6 | 2.75 ± 0.37c | 3.76 ± 0.41a |
Gm6 lines | Bph6+Pigm | 8 | 3.56 ± 0.54b | 3.62 ± 0.42a |
Gm9 lines | Bph9+Pigm | 10 | 3.26 ± 0.19b | 3.43 ± 0.52a |
R6888 | Pigm | 8.20 ± 0.26a | 3.63 ± 0.41a | |
Luoyang69 | Bph6+Bph9 | 2.73 ± 0.15c | 8.23 ± 0.15b | |
9311 | 7.90 ± 0.16a | 8.33 ± 0.33b |
Line | GY (t/ha) | DTH (d) | PH (cm) | PL (cm) | NGP | SF (%) | TGW (g) | AAC (%) | GC (mm) | GT | HRR (%) | LWR | CD (%) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Huahui3006 | 8.43 | 125 | 114.7 | 23.8 | 220.6 | 88.90 | 27.5 | 16.10 | 62 | 6.0 | 64.20 | 3.4 | 3.60 |
Huahui7713 | 8.67 | 126 | 114.3 | 23.2 | 230.6 | 88.20 | 27.8 | 16.40 | 63 | 6.0 | 63.10 | 3.3 | 4.60 |
R6888 | 8.31 | 129 | 117.6 | 22.3 | 258.2 | 85.80 | 27.3 | 15.50 | 65 | 3.0 | 64.10 | 3.3 | 4.41 |
Luoyang69 | 8.52 | 120 | 112.3 | 23.8 | 171.4 | 88.01 | 29.4 | 17.30 | 65 | 6.2 | 69.50 | 2.9 | 7.05 |
Line | Genotype | Blast resistance | BPH resistance | |||||||
Bph6 | Bph9 | Pigm | Seedling | Panicle blast severity index | Incidence of infected panicles | Integrated resistance index | ||||
R6888 | - | - | + | 3 | 3 | 3 | 3 | HS | ||
Luoyang69 | + | + | - | 9 | 9 | 9 | 9 | R | ||
Huahui3006 | + | + | + | 3 | 3 | 3 | 3 | R | ||
Huahui7713 | + | + | + | 3 | 3 | 3 | 3 | R | ||
XuanLiangYou3006 | +/- | +/- | +/- | 3 | 3 | 3 | 3 | R | ||
WeiLiangYou7713 | +/- | +/- | +/- | 3 | 3 | 3 | 3 | R |
Variety | Year | Agronomic traits | Grain quality traits | Blast resistance | BPH resistance | |||||||||||
GY | DTM | AAC (%) | GC (mm) | CD (%) | HRR (%) | IDI | HSPBS | |||||||||
GY (t/ha) | ±CK (%) | DTM (d) | ±CK (d) | |||||||||||||
Weiliangyou7713 | 2019 | 10.22 | 6.27 | 138.0 | 4.6 | 15 | 82 | 0.8 | 68.9 | 3.5 | 5 | R | ||||
2020 | 9.61 | 8.76 | 135.9 | 2.8 | 14.3 | 82 | 0.9 | 65.9 | 3 | 5 | R | |||||
Average | 9.91 | 7.52 | 136.9 | 3.7 | 14.65 | 82 | 0.85 | 67.4 | 3.25 | 5 | R | |||||
Xuanliangyou3006 | 2020 | 9.13 | 3.24 | 137.5 | 5.6 | 16.8 | 60 | 1.4 | 66.3 | 3.4 | 3 | MR | ||||
2021 | 9.39 | 1.89 | 139.0 | 8.1 | 15.4 | 61 | 7 | 64.9 | 2.6 | 3 | MR | |||||
Average | 9.26 | 2.56 | 138.3 | 6.9 | 16.1 | 60.5 | 4.2 | 65.6 | 3 | 3 | MR |
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