Mashela, P.; Pofu, K.M.; Ndhlala, A.R. Nemafric-BL Phytonematicide Induces Isoprenylation and Farneslation in Guava Root-Knot Nematode, Meloidogyne enterolobii: Mechanism of Cucurbitacin-Cuticle Interaction. Preprints2024, 2024031512. https://doi.org/10.20944/preprints202403.1512.v1
APA Style
Mashela, P., Pofu, K.M., & Ndhlala, A.R. (2024). Nemafric-BL Phytonematicide Induces Isoprenylation and Farneslation in Guava Root-Knot Nematode, <em>Meloidogyne enterolobii</em>: Mechanism of Cucurbitacin-Cuticle Interaction. Preprints. https://doi.org/10.20944/preprints202403.1512.v1
Chicago/Turabian Style
Mashela, P., Kgabo Martha Pofu and Ashwell Rungano Ndhlala. 2024 "Nemafric-BL Phytonematicide Induces Isoprenylation and Farneslation in Guava Root-Knot Nematode, <em>Meloidogyne enterolobii</em>: Mechanism of Cucurbitacin-Cuticle Interaction" Preprints. https://doi.org/10.20944/preprints202403.1512.v1
Abstract
Cucurbitacin phytonematicides consistently suppress population densities of plant nematodes, but have no effect against bacteria, fungi and insects. This study investigates the mechanism through which cucurbitacin phytonematicides consistently exert nematicidal properties on nematodes. Post 72-h exposure of second-stage juveniles (J2) of Meloidogyne enterolobii to geometric concentration of Nemafric-BL phytonematicide, J2 total proteins were measured using TruspecCHNS Macro. Total proteins versus phytonematicide exhibited a negative quadratic relation (R2 = 0.97), with the minimum total proteins achieved at x = 4.9% phytonematicide. Beyond the minimum, total proteins increased gradually, depicting the existence of both isoprenylation (breakdown) and farnesylation (biosynthesis) of total proteins. In conclusion, cucurbitacins and nematode cuticles are both chemically structured, which provides the mechanism through which the test chemicals consistently decrease population densities of plant nematodes.
Keywords
biosorption; CARD; cucurbitacins; cuticles; farneslation; isoprenylation; Meloidogyneenterolobii; total protein
Subject
Biology and Life Sciences, Life Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
