Double Targeting Inflammasome Activation and Accelerated Cellular Senescence in Alveolar Macrophages Exposed to Cigarette Smoke and COPD

Patrick Fordjour Asare; Greg Hodge; Miranda Ween; Rhys Hamon; Tung Thanh Truong; Hubertus Jersmann; Paul Reynolds; Sandra Hodge; Hai Bac Tran

doi:10.20944/preprints202403.1380.v2

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preprints.org > biology and life sciences > aging > doi: 10.20944/preprints202403.1380.v2

Preprint Article Version 2 Preserved in Portico This version is not peer-reviewed

Double Targeting Inflammasome Activation and Accelerated Cellular Senescence in Alveolar Macrophages Exposed to Cigarette Smoke and COPD

Patrick Fordjour Asare

Version 1 : Received: 22 March 2024 / Approved: 22 March 2024 / Online: 25 March 2024 (07:38:11 CET)
Version 2 : Received: 22 March 2024 / Approved: 25 March 2024 / Online: 25 March 2024 (08:49:57 CET)

How to cite: Asare, P.F.; Hodge, G.; Ween, M.; Hamon, R.; Truong, T.T.; Jersmann, H.; Reynolds, P.; Hodge, S.; Tran, H.B. Double Targeting Inflammasome Activation and Accelerated Cellular Senescence in Alveolar Macrophages Exposed to Cigarette Smoke and COPD. Preprints 2024, 2024031380. https://doi.org/10.20944/preprints202403.1380.v2 Asare, P.F.; Hodge, G.; Ween, M.; Hamon, R.; Truong, T.T.; Jersmann, H.; Reynolds, P.; Hodge, S.; Tran, H.B. Double Targeting Inflammasome Activation and Accelerated Cellular Senescence in Alveolar Macrophages Exposed to Cigarette Smoke and COPD. Preprints 2024, 2024031380. https://doi.org/10.20944/preprints202403.1380.v2

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MDPI and ACS Style

Asare, P.F.; Hodge, G.; Ween, M.; Hamon, R.; Truong, T.T.; Jersmann, H.; Reynolds, P.; Hodge, S.; Tran, H.B. Double Targeting Inflammasome Activation and Accelerated Cellular Senescence in Alveolar Macrophages Exposed to Cigarette Smoke and COPD. Preprints 2024, 2024031380. https://doi.org/10.20944/preprints202403.1380.v2

APA Style

Asare, P.F., Hodge, G., Ween, M., Hamon, R., Truong, T.T., Jersmann, H., Reynolds, P., Hodge, S., & Tran, H.B. (2024). Double Targeting Inflammasome Activation and Accelerated Cellular Senescence in Alveolar Macrophages Exposed to Cigarette Smoke and COPD. Preprints. https://doi.org/10.20944/preprints202403.1380.v2

Chicago/Turabian Style

Asare, P.F., Sandra Hodge and Hai Bac Tran. 2024 "Double Targeting Inflammasome Activation and Accelerated Cellular Senescence in Alveolar Macrophages Exposed to Cigarette Smoke and COPD" Preprints. https://doi.org/10.20944/preprints202403.1380.v2

Abstract

We hypothesise that anti-inflammatory macrolides can alleviate both the NLRP3 inflammasome activation and accelerated senescence in alveolar macrophages in COPD and/or response to cigarette smoke. Lung tissues from COPD patients and mice chronically exposed to cigarette smoke, cultures of human primary alveolar macrophages and macrophages derived from blood (MDM) or THP-1 monocytes were analysed for markers of macrophage NLRP3 inflammasome activation and accelerated senescence using multifluorescence quantitative confocal microscopy, Western blot, flow cytometry and histochemistry. Cultured macrophages were stimulated with 10% cigarette smoke extract (CSE), with or without presence of non-antibiotic macrolides 2'-desoxy-9-(S)-erythromycylamine and azithromycin-based 2'-desoxy molecule. Cigarette smoke and CSE induced in macrophages significant (p

Keywords

inflammasome; NLRP3; cellular senescence; antiinflammatory macrolides; alveolar macrophages; COPD

Subject

Biology and Life Sciences, Aging

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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