Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Effects of low and high maternal protein intake on fetal skeletal muscle miRNAome in sheep

Bilal Akyüz
,
Md Mahmodul Hasan Sohel
,
Yusuf Konca
ORCID logo ,
Korhan Arslan
,
Kutlay Gürbulak
,
Murat Abay
ORCID logo ,
Mahmut Kaliber
,
Stephen N. White
,
Mehmet Ulas Cinar
* ORCID logo
Version 1 : Received: 20 March 2024 / Approved: 20 March 2024 / Online: 20 March 2024 (13:29:15 CET)

How to cite: Akyüz, B.; Sohel, M.M.H.; Konca, Y.; Arslan, K.; Gürbulak, K.; Abay, M.; Kaliber, M.; White, S.N.; Cinar, M.U. Effects of low and high maternal protein intake on fetal skeletal muscle miRNAome in sheep. Preprints 2024, 2024031208. https://doi.org/10.20944/preprints202403.1208.v1 Akyüz, B.; Sohel, M.M.H.; Konca, Y.; Arslan, K.; Gürbulak, K.; Abay, M.; Kaliber, M.; White, S.N.; Cinar, M.U. Effects of low and high maternal protein intake on fetal skeletal muscle miRNAome in sheep. Preprints 2024, 2024031208. https://doi.org/10.20944/preprints202403.1208.v1

Abstract

Prenatal maternal feeding plays an important role in fetal development and has potential to cause long-lasting epigenetic modifications. MicroRNAs (miRNAs) are non-coding, single-stranded RNAs that serve as one epigenetic mechanism. Though miRNAs have crucial roles in fetal programming, growth, and development, there are limited data regarding maternal diet and sheep miRNA expression. Therefore, weanalyzed high and low maternal dietary protein for miRNA expression in sheep fetal longissimus dorsi. Pregnant ewes were fed an isoenergetic high-protein (HP, 160–270 g/day), low-protein (LP, 73–112 g/day), or standard protein diet (SP, 119–198 g/day) during pregnancy. miRNA expression profiles were evaluated using the Affymetrix GeneChip miRNA 4.0 Array. Twelve up-regulated, differentially expressed miRNAs (DE miRNAs) were identified targeting 65 genes. The oar-3957-5p miRNA was highly up-regulated in LP and SP compared to HP. Previous transcriptome analysis identified integrin and non-receptor protein tyrosine phosphatase genes targeted by miRNAs detected in the current experiment. A total of 28 GO terms and 10 pathway-based gene sets were significantly (padj < 0.05) enriched in target genes. Most genes targeted by identified miRNAs are involved in immune and muscle disease pathways. Our study shows dietary protein intake during pregnancy affects fetal skeletal muscle epigenetics via miRNA expression.

Keywords

Ovis aries; nutrimiromics; dietary protein; prenatal life; gestation

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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