Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Diagnostic Accuracy of Saliva-Based Testing as a Vibrio cholerae Surveillance Tool

Caroline Cleopatra Chisenga
ORCID logo ,
Bernard Phiri
,
Harriet Ng'ombe
,
Mutinta Muchimba
ORCID logo ,
Fraser Liswaniso
ORCID logo ,
Biana Bernshtein
,
Adam F Cunningham
,
David Sack
,
Samuel Bosomprah
*
Version 1 : Received: 5 March 2024 / Approved: 7 March 2024 / Online: 8 March 2024 (03:52:16 CET)

How to cite: Chisenga, C.C.; Phiri, B.; Ng'ombe, H.; Muchimba, M.; Liswaniso, F.; Bernshtein, B.; Cunningham, A.F.; Sack, D.; Bosomprah, S. Diagnostic Accuracy of Saliva-Based Testing as a Vibrio cholerae Surveillance Tool. Preprints 2024, 2024030463. https://doi.org/10.20944/preprints202403.0463.v1 Chisenga, C.C.; Phiri, B.; Ng'ombe, H.; Muchimba, M.; Liswaniso, F.; Bernshtein, B.; Cunningham, A.F.; Sack, D.; Bosomprah, S. Diagnostic Accuracy of Saliva-Based Testing as a Vibrio cholerae Surveillance Tool. Preprints 2024, 2024030463. https://doi.org/10.20944/preprints202403.0463.v1

Abstract

Saliva, as a diagnostic medium, offers a promising alternative to blood by virtue of its non-invasive collection, which enhances patient compliance, especially in paediatric and geriatric populations. In this study, we assessed the utility of saliva as a non-invasive medium for measuring V. cholerae-specific serum antibodies in naturally infected individuals. We tested paired serum and saliva samples obtained from a total of 63 cholera patients enrolled in a cohort study. Vibriocidal antibodies assay (IgM/IgG) as markers for accurate determination was used to determine cholera specific antibody levels. Using receiver operating characteristics (ROC) curve, we found that the best cut-off that maximizes (sensitivity + specificity) is 10 titers. At this saliva titer, the sensitivity is 76.9% (95%CI: 60.9%, 87.7%) and specificity is 80.0% (95%CI: 56.6%, 92.5%). Using Spearman’s correlation coefficient, we also found evidence of a positive correlation between Vibrio Cholerae saliva and serum antibodies (rho=0.66, p<0.001). In conclusion, saliva-based diagnostic cholera tests has high diagnostic accuracy, and would be advantageous, cheaper, and quicker for early diagnosis of severe cholera outcomes.

Keywords

saliva; vibriocidal antibodies; Cholera; natural infection

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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