Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Human Dermal Decellularized ECM Hydrogels as Scaffolds for 3D In Vitro Skin aging Models

Estibaliz Fernandez-Carro
ORCID logo ,
Ana Rosa Remacha
,
Irene Orera
ORCID logo ,
Giuseppe Lattanzio
,
Alberto Garcia-Barrios
ORCID logo ,
Jesús Del Barrio
,
Clara Alcaine
,
Jesús Ciriza
*
Version 1 : Received: 7 March 2024 / Approved: 7 March 2024 / Online: 7 March 2024 (14:20:02 CET)

A peer-reviewed article of this Preprint also exists.

Fernandez-Carro, E.; Remacha, A.R.; Orera, I.; Lattanzio, G.; Garcia-Barrios, A.; del Barrio, J.; Alcaine, C.; Ciriza, J. Human Dermal Decellularized ECM Hydrogels as Scaffolds for 3D In Vitro Skin Aging Models. Int. J. Mol. Sci. 2024, 25, 4020. Fernandez-Carro, E.; Remacha, A.R.; Orera, I.; Lattanzio, G.; Garcia-Barrios, A.; del Barrio, J.; Alcaine, C.; Ciriza, J. Human Dermal Decellularized ECM Hydrogels as Scaffolds for 3D In Vitro Skin Aging Models. Int. J. Mol. Sci. 2024, 25, 4020.

Abstract

Biomaterials play an important role in the development of advancing three dimensional (3D) in vitro skin models, providing valuable insights for drug testing and tissue-specific modeling. Commercial materials, such as collagen, fibrin or alginate, have been widely used in skin modelling. However, they do not adequately represent the molecular complexity of skin components. On this regard, the development of novel biomaterials that represent the complexity of tissues are becoming more important in the design of advanced models. In this study, we have obtained aged human decellularized dermal extracellular matrix (dECM) hydrogels extracted from cadaveric human skin and demonstrated their potential as scaffold for advanced skin models. These dECM hydrogels effectively reproduce the complex fibrillar structure of other common scaffolds, exhibiting similar mechanical properties, while preserving the molecular composition of the native dermis. It is worth noting that fibroblasts embedded within human dECM hydrogels exhibit behavior more representative of natural skin compared to commercial collagen hydrogels, where uncontrolled cell proliferation leads to material shrinkage. The described human dECM hydrogel is able to be used as scaffold for dermal fibroblasts in a skin aging on chip model. These results demonstrate that dECM hydrogels preserve essential components of the native human dermis making them a suitable option for the development of 3D skin aging models that accurately represent the cellular microenvironment, improving existing in vitro skin models and allowing for more reliable results in dermatopathological studies.

Keywords

Biomaterials; decellularization; human dermal extracellular matrix hydrogels; skin aging on chip; microfluidics

Subject

Biology and Life Sciences, Aging

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF Download Supplementary

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Supplementary Files Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.