PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Optical Insight: Spectrophotometry as a Tool to Quantify Cell Density of Green Microalgae in Suspension, with Emphasis on Growing Conditions and Toxicological Evaluations
Version 1
: Received: 4 March 2024 / Approved: 5 March 2024 / Online: 5 March 2024 (15:00:27 CET)
How to cite:
Pessoa, J.D.S.; Almeida, A.C.O.D.; Santos, L.F.D.; Oliveira, T.M.N.D.; Martins, C.C.; Matias, W.G.; Melegari, S.P. Optical Insight: Spectrophotometry as a Tool to Quantify Cell Density of Green Microalgae in Suspension, with Emphasis on Growing Conditions and Toxicological Evaluations. Preprints2024, 2024030275. https://doi.org/10.20944/preprints202403.0275.v1
Pessoa, J.D.S.; Almeida, A.C.O.D.; Santos, L.F.D.; Oliveira, T.M.N.D.; Martins, C.C.; Matias, W.G.; Melegari, S.P. Optical Insight: Spectrophotometry as a Tool to Quantify Cell Density of Green Microalgae in Suspension, with Emphasis on Growing Conditions and Toxicological Evaluations. Preprints 2024, 2024030275. https://doi.org/10.20944/preprints202403.0275.v1
Pessoa, J.D.S.; Almeida, A.C.O.D.; Santos, L.F.D.; Oliveira, T.M.N.D.; Martins, C.C.; Matias, W.G.; Melegari, S.P. Optical Insight: Spectrophotometry as a Tool to Quantify Cell Density of Green Microalgae in Suspension, with Emphasis on Growing Conditions and Toxicological Evaluations. Preprints2024, 2024030275. https://doi.org/10.20944/preprints202403.0275.v1
APA Style
Pessoa, J.D.S., Almeida, A.C.O.D., Santos, L.F.D., Oliveira, T.M.N.D., Martins, C.C., Matias, W.G., & Melegari, S.P. (2024). Optical Insight: Spectrophotometry as a Tool to Quantify Cell Density of Green Microalgae in Suspension, with Emphasis on Growing Conditions and Toxicological Evaluations. Preprints. https://doi.org/10.20944/preprints202403.0275.v1
Chicago/Turabian Style
Pessoa, J.D.S., William Gerson Matias and Silvia P. Melegari. 2024 "Optical Insight: Spectrophotometry as a Tool to Quantify Cell Density of Green Microalgae in Suspension, with Emphasis on Growing Conditions and Toxicological Evaluations" Preprints. https://doi.org/10.20944/preprints202403.0275.v1
Abstract
Microalgae are highly attractive to various industrial sectors and can serve as indicators of aquatic environmental health. An essential step for cultivating microalgae is assessing the cell density, which is traditionally performed through viable cell counting by optical microscopy (OM). However, this method has some limitations, such as the time involved in the process of counting, and the need for specialized training of laboratory personnel. Spectrophotometry is an affordable alternative, especially when applied to culture growth and toxicological tests. We chose the wavelength of 680 nm to detect the optical density (OD) of the green freshwater microalga Desmodesmus subspicatus and green the marine microalga Tetraselmis sp. The correlation between the number of cells and OD was suitable for both microalgae and accuracy was comparable to traditional OM counting. In toxicological tests, the OD technique allowed the monitoring of microalgal growth in the presence of the chemicals potassium dichromate, potassium chloride and sodium dodecyl sulfate (SDS), with results very close to those reported in the scientific literature. This study sheds light on this well-known technique, which is an important contribution since few articles have explored its potentialities and fragilities, showing the successful application to monitoring green microalgal cultures and toxicological assays.
Environmental and Earth Sciences, Environmental Science
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
