Whitman, J.A.; Doherty, L.A.; Pantoja-Feliciano de Goodfellow, I.G.; Racicot, K.; Anderson, D.J.; Kensil, K.; Karl, J.P.; Gibson, G.R.; Soares, J.W. In Vitro Fermentation Shows Polyphenol and Fiber Blends Have an Additive Beneficial Effect on Gut Microbiota States. Nutrients2024, 16, 1159.
Whitman, J.A.; Doherty, L.A.; Pantoja-Feliciano de Goodfellow, I.G.; Racicot, K.; Anderson, D.J.; Kensil, K.; Karl, J.P.; Gibson, G.R.; Soares, J.W. In Vitro Fermentation Shows Polyphenol and Fiber Blends Have an Additive Beneficial Effect on Gut Microbiota States. Nutrients 2024, 16, 1159.
Whitman, J.A.; Doherty, L.A.; Pantoja-Feliciano de Goodfellow, I.G.; Racicot, K.; Anderson, D.J.; Kensil, K.; Karl, J.P.; Gibson, G.R.; Soares, J.W. In Vitro Fermentation Shows Polyphenol and Fiber Blends Have an Additive Beneficial Effect on Gut Microbiota States. Nutrients2024, 16, 1159.
Whitman, J.A.; Doherty, L.A.; Pantoja-Feliciano de Goodfellow, I.G.; Racicot, K.; Anderson, D.J.; Kensil, K.; Karl, J.P.; Gibson, G.R.; Soares, J.W. In Vitro Fermentation Shows Polyphenol and Fiber Blends Have an Additive Beneficial Effect on Gut Microbiota States. Nutrients 2024, 16, 1159.
Abstract
Polyphenols and fermentable fibers have shown favorable effects on gut microbiota composition and metabolic function. However, few studies have investigated whether combining multiple fermentable fibers or polyphenols may have additive beneficial effects on gut microbial states. Here, an in vitro fer-mentation model, seeded with human stool combined from 30 healthy volunteers, was supplemented with blends of polyphenols (PP), dietary fibers (FB) or their combination (PPFB) to determine influence on gut bacteria growth dynamics and select metabolite changes. PP and FB blends independently lead to significant increases in the absolute abundance of select beneficial taxa, namely Ruminococcus bromii, Bifidobacterium spp., Lactobacillus spp. and Dorea spp. Total short chain fatty acid concentrations, relative to non-supplemented control (F), increased significantly with PPFB and FB supplementation, but not PP. Indole and ammonia concentrations decreased with FB and PPFB supplementation, but not PP alone while increased antioxidant capacity was only evident with both PP and PPFB. These findings demonstrated that, while the independent blends displayed selective positive impacts on gut states, combination of both blends provided an additive effect. The work outlines the potential of mixed substrate blends to elicit a broader positive influence on gut microbial composition and function to build resiliency toward dysbiosis.
Keywords
prebiotics; gut microbiome; carbohydrate; supplementation; bacteria; extracts; metabolites
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
