Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Fluorescently Tagged Verticillium dahliae to Understand the Infection Process on Cotton (Gossypium hirsutum) and Its Survival on Other Plants including Weed Species

Andrew Chen
* ORCID logo ,
Sabrina Morrison
,
Aphrika Gregson
,
Duy P. Le
,
Andrew S. Urquhart
,
Linda J. Smith
ORCID logo ,
Elizabeth A.B. Aitken
* ,
Donald M. Gardiner
* ORCID logo
Version 1 : Received: 1 March 2024 / Approved: 1 March 2024 / Online: 1 March 2024 (10:42:29 CET)

How to cite: Chen, A.; Morrison, S.; Gregson, A.; Le, D.P.; Urquhart, A.S.; Smith, L.J.; Aitken, E.A.; Gardiner, D.M. Fluorescently Tagged Verticillium dahliae to Understand the Infection Process on Cotton (Gossypium hirsutum) and Its Survival on Other Plants including Weed Species. Preprints 2024, 2024030031. https://doi.org/10.20944/preprints202403.0031.v1 Chen, A.; Morrison, S.; Gregson, A.; Le, D.P.; Urquhart, A.S.; Smith, L.J.; Aitken, E.A.; Gardiner, D.M. Fluorescently Tagged Verticillium dahliae to Understand the Infection Process on Cotton (Gossypium hirsutum) and Its Survival on Other Plants including Weed Species. Preprints 2024, 2024030031. https://doi.org/10.20944/preprints202403.0031.v1

Abstract

Verticillium wilt is a soil borne disease caused by distinct vegetative compatibility groups (VCG) of the fungus Verticillium dahliae. Two V. dahliae isolates were recovered from symptomatic cot-ton plants in Australian cotton fields and were assigned to two distinct vegetative compatibility groups (VCGs). One corresponding to the defoliating (VCG 1A) and the other non-defoliating (VCG 2A) pathotypes of Verticillium, have been transformed with Red and Green Fluorescent protein genes, respectively. The transformants maintained their ability to infect cotton and both strains were observed to colonise the xylem vessels of cotton plants. Moreover, we observed that the cotton V. dahliae strains could also infect some sampled non-Gossypium species found in the Australian landscape. The fluorescently labelled strains of V. dahliae will allow us to gain a thorough understanding of the infection processes of this important pathogen.

Keywords

Reporter genes; plant host and pathogen interactions; Green Fluorescent Protein; 'mCherry' Red Fluorescent Protein; alternative weed host; Nicotina benthamiana; Vegetative Compatibility Groups 1A and 2A; Defoliating and non-defoliating pathotypes

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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