Article
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Malian children core gut mycobiome
Version 1
: Received: 29 February 2024 / Approved: 29 February 2024 / Online: 29 February 2024 (17:02:42 CET)
How to cite: Abdillah, A.; Aly, K.; Ranque, S. Malian children core gut mycobiome. Preprints 2024, 2024021756. https://doi.org/10.20944/preprints202402.1756.v1 Abdillah, A.; Aly, K.; Ranque, S. Malian children core gut mycobiome. Preprints 2024, 2024021756. https://doi.org/10.20944/preprints202402.1756.v1
Abstract
Because data on the fungal gut community structure of African children are scarce, we aimed to describe by reanalysing rRNA ITS1 and ITS2metabarcoding data from a study designed to assess the influence of microbiota in malaria susceptibility in Malian children from the Dogon country. More specifically, we aimed to establish the core gut mycobiome and compare the gut fungal community structure of breastfed children, aged 0-2 years, with other age groups.
Briefly, DNA was extracted from 296 children’s stool samples. Both rRNA ITS1 and ITS2 genomic barcodes were amplified and subjected to Illumina MiSeq sequencing.
The ITS2 barcode generated 1,975,320 reads and 532 operational taxonomic units (OTUs), while the ITS1 barcode generated 647,816 reads and 532 OTUs. The alpha diversity was significantly higher by using the ITS1 compared to the ITS2 barcode (p
Keywords
Fungal gut community structure; gut mycobiome; core mycobiome; ITS metabarcoding; children; Dogon country
Subject
Biology and Life Sciences, Immunology and Microbiology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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