Song, X.; Cao, Y.; Yan, F. Isothermal Nucleic Acid Amplification-Based Lateral Flow Testing for the Detection of Plant Viruses. Int. J. Mol. Sci.2024, 25, 4237.
Song, X.; Cao, Y.; Yan, F. Isothermal Nucleic Acid Amplification-Based Lateral Flow Testing for the Detection of Plant Viruses. Int. J. Mol. Sci. 2024, 25, 4237.
Song, X.; Cao, Y.; Yan, F. Isothermal Nucleic Acid Amplification-Based Lateral Flow Testing for the Detection of Plant Viruses. Int. J. Mol. Sci.2024, 25, 4237.
Song, X.; Cao, Y.; Yan, F. Isothermal Nucleic Acid Amplification-Based Lateral Flow Testing for the Detection of Plant Viruses. Int. J. Mol. Sci. 2024, 25, 4237.
Abstract
Isothermal Nucleic Acid Amplification based-Lateral Flow Testing (INAA-LFT) has emerged as a robust technique for on-site pathogen detection, providing a visible indication of pathogen nucleic acid amplification that rivals or even surpasses the sensitivity of real-time quantitative PCR. The isothermal nature of INAA-LFT ensures consistent conditions for nucleic acid amplification, establishing it as a crucial technology for rapid on-site pathogen detection. However, despite its considerable promise, the widespread application of isothermal INAA amplification-based lateral flow testing faces several challenges. This review provides an overview of the INAA-LFT procedure, highlighting its advancements in detecting plant viruses. Moreover, the review underscores the imperative to address existing limitations and emphasizes ongoing research efforts dedicated to enhancing the applicability and performance of this technology in the realm of rapid on-site testing.
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
